We narrowed to 304 results for: human dat

...medium during adeno-associated virus manufacturing." Human gene therapy21.10 (2010): 1251-1257. PMID: 20649475...production strategies or developing novel methods for data analysis, our team’s goal is to provide scientists...

...Oliver Sacks examines the relationship between the human brain and music: how we perceive it, why it affects...of southern England. While the length may be intimidating, Ruthefurd’s writing will capture the attention...favorite books” to “I barely got through it.”  To date, we’ve read 76 books for ABC. Some are easy reads...

...match variations present in the data such as synapsin, hsyn synapsin-1, human synapsin, hSynap. Matching ...processes to curate the data our depositors share and enrich it with sequencing data; as a result, the materials... including the recent homepage and search tool updates. Now, let’s take a look at the least visible part...cross-team team, with members from the Customer Service, Data Curation, Outreach, Product, Scientist, and Software... menu. For users who prefer to use search, our updated search engine is now more powerful, intuitive, ...knowledge of the scientific domain in general and of our data in particular and our deep understanding of Addgene...and synonyms of the same term that appear in our data into a single filter value. For example, there is...

...adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate. Image courtesy of the Gradinaru Lab and...review performance data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech...

...a 9 amino acid peptide (YPYDVPDYA) derived from human influenza hemagglutinin. The tag has two negatively... brought about by the tag so it’s important to validate tagged protein functionality (Arribere, et al)...

...0.66 million targetable NGG PAM sites whereas the human genome has about 300 million. With such a sparsely.... Ghorbal, Mehdi, et al. "Genome editing in the human malaria parasite Plasmodium falciparum using the...separate sgRNA design tools into database and de novo solutions. Database tools allow us to view and get...a good example of how database tools can be used to aid design. These databases are still young and will...usually the target site. Multi-locus asseEENdb database ssment would be required to fully evaluate off-targeting...rational design. The first of only three such databases, EENdb (published 1 Jan 2013, no longer available...catalogue of reported sgRNA designs. The second database, CrisprGE (published 27 Jun 2015), is noteworthy...

...Richie lab are especially useful for studies in non-human primates, where fusion fluorescent proteins can ...quality control. For an estimate on availability date or to be notified when a particular catalog item...

...re-amplified our distribution stock of the Brunello Human gRNA library, and we thought it would be a good ... Topic Page Learn How We Use NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries...

... for expression in Cre-expressing cells hSynap: Human Synapsin-1 promoter. Good for neuronal expression...Ford in 2018. It was updated by guest blogger Abhi Aggarwal in 2022.  Recent updates to iGluSnFR and SF-iGluSnFR...SF-iGluSnFR have made it clear that it’s time to update our iGluSnFR post! Here, we look at the origins...

...complications of diabetes mellitus. Recombinant human proteins that play a role in insulin regulation ...complications of diabetes Gloria Otito Izu, PhD Candidate, University of Ibadan, Nigeriaunder supervision... of Ibadan, Nigeria Gloria Otito Izu is a PhD Candidate working under the supervision of Akindele Oluwatosin...projects progress. Subscribe to the Addgene blog for updates and future collaborations. Many thanks to all of...

...choose a lab that works on problems pertinent to human disease. For more details and many more suggestions...needs. These two factors will provide a solid foundation on which to thrive during time spent as a graduate...also includes a useful list of questions for a candidate to ask lab heads, lab members and lab neighbors...watch the webinar "How to Choose Your Next Lab" (updated 2020 version) by Addgene Executive Director, Joanne...

...our users, we now provide the Brunello (targeting human genes) and Brie (targeting mouse genes) pooled libraries...with all of our viral preps and host our expression data (western blots or fluorescent microscopy) on the...these widely studied signalling pathways. Find Validated gRNAs to use with your Cas9 Virus Pooled libraries...

...of technologies to functionally investigate the human genome. Doench says he enjoys working on technology...still gets to see data as it’s coming out and says “That first glimpse of what the data look like... when...

...in metazoans. The superfamily is comprised of 48 human and 49 murine members. NRs permit the integration...coregulator-related global gene profiling datasets in to our data mining tool, Transcriptomine. We are currently...links to datasets and reagents in the field. Additional information can be found in the Datasets and Reagents...research, and has generated a set of comprehensive datasets that collectively address many important and fundamental...nuclear receptor and coregulator research. Our Q-PCR datasets, for example, document the expression patterns...seeking to compliment these consortium-generated datasets with a systematic effort to compile NR- and coregulator-related...

...opportunities. In an age of scarcity, the rational human being will tend to clinch every opportunity for ...resources, we created STEAMid, for a nationwide database of  STEAM internships for high school, college... a solid personal statement.  4. Letters of recommendation wisdom  Reach out to 2-3 individuals in your...applications. Thus these noble individuals are inundated with requests. The easier you make it for them...Roodolph (Roo) P. St Pierre is currently a doctoral candidate in the Chemical Biology Program at Harvard. Roo...

... it was discovered that SARS-CoV, which entered human cells via hACE2, could not enter cells expressing...research because they provide rapid access to validated reagents, especially during a time when these ... maintain repositories’ capacity for storing, validating, and distributing such reagents are all ways ...on the Addgene blog Read more about COVID-19 databases and resources Learn about Addgene's work to share...

...guide RNAs enhance CRISPR-Cas genome editing in human primary cells. Nat Biotechnol 33, 985–989 (2015)..., gRNAs can be chemically modified to resist degradation while still effectively serving their function...recognized as foreign RNA. To protect the RNA from degradation, several simple methods have been employed which...stability.  Nature has evolved ways to prevent degradation of important cellular RNA molecules with post...

...paper Systematic morphological profiling of human gene and allele function via Cell Painting. Rohban...mutations that are present, and use prior knowledge (data connecting each genotype with vulnerabilities) to...thus providing a starting point for downstream validation experiments. But, there have been two huge challenges... exciting plasmid collections (together with the data generated from these alleles in the above publications...

...to demonstrate that this approach was useful in humans. Then, one of them heard about our online platform...protocol can save weeks of trial-and-error. A recommendation for the correct statistical test can save a...

...collection reported to date, containing all 620 sequences of probable mature human miRNAs. Weiss’s collection...mechanisms of miRNA regulation have yet to be fully elucidated. Ron Weiss’s lab at MIT pushed the field further...