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Plasmids for Endogenous Gene Tagging in Human Cells

Type

Blog Post

...plasmid backbone for delivery to the cells. As a precautionary measure, it’s also good to make a few single...
Hot Plasmids - October 2020

Type

Blog Post

...al., 2020. The researchers tested several scenarios from embryo development to myeloid proliferation...
How to Prepare for an Industry Interview

Type

Blog Post

... get ready for this interview? Maybe the above scenario sounds familiar to you. It was my experience not...
A Look Back at One Year of Plasmid Sharing for COVID-19 Research

Type

Blog Post

..., Wang N, Amengor D, Lavinder JJ, Ippolito GC, Maynard JA, Finkelstein IJ, McLellan JS (2020) Structure-based...
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

Type

Blog Post

...CL, Jones B, Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville...
High Complexity Golden Gate Assembly with NEB’s DAD

Type

Blog Post

...specificity, you can exclude specific sequences or set a narrow search window for a site—or sites—that must be ...
Science communication: 9 Strategies to Get Your Foot in the Door

Type

Blog Post

...and bananas. You can follow her on Twitter @BananarootsBlog. Additional Resources on the Addgene Blog...
Cpf1 Update: Comparison to Cas9 and NgAgo

Type

Blog Post

...der Oost J, Backofen R, Koonin EV. An updated evolutionary classification of CRISPR-Cas systems. 5. Nat...
Plasmids 101: FLEx Vectors

Type

Blog Post

...plasmids from these Addgene depositors: Ed Boyden Bernardo Sabatini Wenbiao Chen Liquin Luo Massimo Scanziani...
Illuminating Epigenetics with A FRET Based Biosensor

Type

Blog Post

...24552514. PubMed Central PMCID: PMC3929550. 3. Kaati, Gunnar, Lars O. Bygren, and Soren Edvinsson. "Cardiovascular...
10 Basic tips for mammalian cell culture

Type

Blog Post

...your workspace properly, but can also disrupt the laminar air flow around your work area. Make sure to only...
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Type

Blog Post

...wall trying to study it? It happens. Is it an evolutionarily conserved gene? Can you find an ortholog in...
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Type

Blog Post

...colorimetric reaction. After the ELISA step, the narrowed-down pool (usually 10-30) of sybody candidates...
Chromoproteins: Colorful Proteins For Molecular Biology Experiments

Type

Blog Post

...anemone's red color can be used for mimicry. Image: Bernard Spragg. Advantages of using chromoproteins for...
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

Type

Blog Post

...single bacterial strain, such as a B. subtilis, or a narrow range of bacterial species, such as the human gut...
Academic vs. Industry Postdocs

Type

Blog Post

...are busy with their pipeline projects. In this scenario, finding someone with whom you can have an in-...
Transferable Skills: Negotiation

Type

Blog Post

...about the individuals, each example provides a scenario where both people get something valuable. Importantly...
Degrading DNA with Cascade-Cas3

Type

Blog Post

..., J., . . . Koonin, E. V. (2015). An updated evolutionary classification of CRISPR–Cas systems. Nature...
The AAVantages of AAV in CRISPR Screens

Type

Blog Post

...Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal, T., Yadanar, L., Tian, R., Ma, K., Palop, J. J., & Kampmann...
Antibodies 101: Conventional vs Spectral Flow Cytometry

Type

Blog Post

...machine uses the full spectral signature, not just a narrow band, to discern one marker from another. Instead...

Showing: 201 - 220 of 275 results

Results per page:

Plasmids for Endogenous Gene Tagging in Human Cells

Hot Plasmids - October 2020

How to Prepare for an Industry Interview

A Look Back at One Year of Plasmid Sharing for COVID-19 Research

Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

High Complexity Golden Gate Assembly with NEB’s DAD

Science communication: 9 Strategies to Get Your Foot in the Door

Cpf1 Update: Comparison to Cas9 and NgAgo

Plasmids 101: FLEx Vectors

Illuminating Epigenetics with A FRET Based Biosensor

10 Basic tips for mammalian cell culture

Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Chromoproteins: Colorful Proteins For Molecular Biology Experiments

Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

Academic vs. Industry Postdocs

Transferable Skills: Negotiation

Degrading DNA with Cascade-Cas3

The AAVantages of AAV in CRISPR Screens

Antibodies 101: Conventional vs Spectral Flow Cytometry

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