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We narrowed to 393 results for: prep

Showing: 201 - 220 of 393 results

  1. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...while meditating right before bed will help you prepare for sleep. At Addgene, our mindfulness group is...more about career paths and things you can do to prepare. Adapt to WFH while parenting Keep your kids...tutorials for all ages. Organize your data and prepare publications or your dissertation Organize data...write introductions to potential publications or prepare a review article. These are both options that can...

  2. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...containing Ad E2A, E4, and VA RNA genes; and the pRepCap plasmid containing replication factors and serotype-specific...been developed to determine the titers of rAAV preparations, such as dot blot, enzyme-linked immunosorbent...overestimate of transducible units in the virus preparation.  rAAVs  Now your AAV virions are ready to store...Unpurified Recombinant Adeno-Associated Viral Vectors [Preprint]. Molecular Biology. https://doi.org/10.1101/2023.03.20.533580...

  3. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...step of the series is consistent at 1:2. When preparing a dilution series, use a new pipette tip for each... could affect the measurements. You'll need to prepare the standard, standard dilutions, and unknown sample...components can affect the final readout. When preparing a standard curve, change pipette tips and mix ...every time the assay is run, ideally with freshly prepared buffer. This is because standard curves vary depending...

  4. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...personality types, and to devise strategies to be prepared for addressing unexpected situations. At the end...long-term? A few years as a healthcare consultant can prepare you for a successful career in several different...foot in the door When I was first considering preparing for and transitioning to a career in healthcare...learning about the nature of the job and the ways to prepare for it. Besides those conversations, reading online...

  5. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...should be consistent over time and from different preparations of the purified test plasmid expressing the ... with purified plasmid from the same aliquot/preparation. The replicate control isn’t measuring or assessing...Using our example above, we would test different preparations of Plasmid A in different aliquots of cells ...singular or associated with only one aliquot/preparation of the test plasmid.  Let us now revisit our ...

  6. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ... can then be purified for subsequent library preparation and high-throughput sequencing. Improvements ...lab Very recently, the Henikoff lab published a preprint paper outlining improvements to the initial CUT... in terms of initial steps of cell or tissue preparation.   All in all, CUT&RUN offers a great alternative...

  7. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...buy a box with the prepaid label and fill it with nitrile gloves, affix the prepaid label, and divert ... (in the U.S.). Their cooler boxes come with a prepaid return shipping label. To return the box, simply...

  8. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...if they have specialized skills and equipment! Preparing for your visit   Design and communicate a research... science usually doesn’t follow our timelines! Prepare a research plan to set yourself up for success ... bench mentor wait on you!), make sure you are prepared (read any protocols the day before, etc.), take...

  9. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...Here are a few things to consider when you're preparing your resume for a non-academic position: What ...services, but some of the general knowledge about preparing a good resume, writing a concise cover letter,..., but the earlier you ask yourself, the better prepared you are for your next step. Here are some great...

  11. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...persuading an employer that your past work has prepared you to contribute at a high level to their team...audience your primary focus When writing a paper or preparing a presentation, it's easy to forget that you have...form? Training in science communication should prepare you to ask and answer these questions for a diverse...

  12. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ... have all of your plasmid data, we’ll send you prepaid shipping materials (i.e., a deposit kit) with instructions... sufficient quantity of DNA is produced from a miniprep and “low copy” if special growth conditions are... you a deposit kit with instructions on how to prepare and ship the samples to Addgene and contact your...

  13. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...region. Thankfully, due to their prominence, the preparation of the fiber optics is probably the least difficult...effect is also an important consideration when preparing the implant itself, which brings me to the second...same considerations apply to the polishing and preparation of the patch cable (although you may be able ...

  14. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...conference, what worked? Use this information to prepare for the next meeting. Navigating talks Before the...publication, consider uploading your work to a preprint server (and include this information on the poster...later on. Update your LinkedIn and your resume and prepare business cards. You might not consider being a ...

  15. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...share- as opposed to sell- science. 4. You can prepare answers beforehand. Joanne answered many questions...incentivize good mentorship: AMA mechanics: Be prepared! The mechanics of doing the AMA were interesting...interesting. I started watching the questions and preparing answers early in the day. Although I didn’t start...

  17. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...fidelity. In this method, substrate pools were prepared consisting of one target (template) strand and... were represented. Sixteen separate pools were prepared, each with a different complement strand covering...applications such as routine cloning or DNA library preparation. However, when a method relies on accurate ligation...

  18. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...complexed with sgRNA. Direct comparison of Cas9 RNPs prepared with an in vitro-transcribed (IVT) sgRNA versus...without investing time and labour on reagent preparation in the lab. Figure 3: csRNP-assisted epitope...temperature for 10 min. Deliver csRNP into cells: Prepare cells for transfection, add csRNP and ssODN to ...

  19. Our New Antibody Mascot is....

    Type
    Blog Post
    ...our new antibody mascot, who's joining us in preparation for our upcoming affinity reagent distribution...quest. Keep an eye out for Abi as they help us prepare for our upcoming antibody launch in late March!...

  20. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...gene expression! We've also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool...Yizhar lab. Addgene is now offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool...
Showing: 201 - 220 of 393 results