We narrowed to 396 results for: promoter
-
TypeBlog Post...straightforward to define, but for control elements such as promoters and transcription terminators, the boundaries ...
-
Visualizing Translation at the Single Molecule Level
TypeBlog Post...fluorescent proteins and is driven by an inducible promoter (Plasmid 64542 or 64543) Before translation, ... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog Post... could be used to supplement newborn diets and promote the formation of a healthy microbiome. In order... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog Post...forms as part of graduation, hiring, funding, and promotion processes, I’m confident that everyone’s enthusiasm... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog Post... Zoom meetings, different tools can be used to promote creative interaction. A great tool that a few people... -
Plasmids 101: Origin of Replication
TypeBlog Post...express massive amounts of protein given the proper promoter and growth conditions. Choose your origin of... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...optimized their initial system by using a stronger promoter and different biochemical secondary energy sources... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog Post...alongside the customer. From there a move with either promotions or further education may allow you to transition... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...will be suitable). For N-terminus tagging, the promoter region of both the alleles should be sequence-...cautiously analyzed for any damage (indels) to the promoter region of the non-HDR allele. For N-terminal tagging... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...You will have the option to indicate separate promoters, tags, or cloning information for each insert.... 8: Add vector backbone information. List any promoters, cloning details for how your gene was inserted... -
Tetracycline Inducible Expression
TypeCollection...may also like... Blog: Inducible Promoters Blog: Repressible Promoters Mammalian shRNA Plasmids Collection...upstream of a minimal CMV promoter, and other tet- or dox-dependent promoters (sometimes generally called... CMV promoter. In the absence of tetracycline, tTA binds to the TRE and its VP16 domain promotes gene ...transactivators and promoters are generally cross-compatible. Your choice of transactivator, promoter, and type ...Considerations Tet Plasmids Inducible and repressible promoters are powerful tools to control gene expression ... tet O sequences with elements like the H1 RNA promoter. And, most importantly, the TetR protein has been...transcriptional activation domain). For simplicity, other promoter elements are not shown. Tetracycline Off (Tet-... -
Brain Initiative Collection
TypeCollection...-mCherry Expression of hM4D(Gi) using the CD68 promoter. Bryan Roth 75033-AAV9 pAAV CD68-hM4D(Gi)-mCherry...mCherry Expression of hM4D(Gi) using the CD68 promoter. Bryan Roth 83894-AAV1 pAAV-hDlx-Flex-dTomato-Fishell...An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed...Cre-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed...recombinase-dependent expression of mNeonGreen from the CAG promoter Viviana Gradinaru 100798-AAV1 pAAV-syn-FLEX-splitTVA-EGFP-tTA...expression of the fluorescent protein EYFP from the CAG promoter Viviana Gradinaru 104055-AAV2 pAAV-CAG-eYFP An...expresses the fluorescent protein eYFP from the CAG promoter Viviana Gradinaru 104055-AAV5 pAAV-CAG-eYFP An... -
Bacterial Expression Systems
TypeCollection...coli strain. Addgene Blog Inducible Promoters Repressible Promoters Synthetic Photobiology: Optogenetics...pRU1701 Promoter activity Fluorescence (GFP+) Gram-negative bacteria Philip Poole 14460 pOT2 Promoter activity...pCHERRY3 Promoter activity Fluorescence (mCherry) Mycobacterium sp. Tanya Parish 24657 pASTA3 Promoter activity...periplasmic localization: PelB, MalE, OmpA ID Plasmid Promoter Tags PI Additional Addgene Protein Purification...Toolkit (Addgene #1000000080) features a library of promoters and purification tags compatible with Golden Gate...temperature, in different bacterial systems. ID Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria...extorquens Julia Vorholt 84693 pMyNT-kan Acetamidase promoter Acetamide Escherichia coli , Mycobacterium smegmatis... -
CRISPR Plasmids - Double-Strand Break (Cut)
TypeCollection...Gene/Insert Promoter PI Publication Return to top Drosophila ID Plasmid Gene/Insert Promoter Selectable...Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable...CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Publication Return to top Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...Return to top Zebrafish ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...top Xenopus ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Parasite ID ...ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene... -
Pushing the Limits of DNA Assembly
TypeBlog Post...assembly reaction. DNA fragments with the desired promoter, selectable marker, and target gene, for example... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...the Francis Crick Institute concluded “Aspirin promotes autophagy, inhibits platelet aggregation, and ... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...antibiotic resistance genes, selectable markers, promoters, origins, tags, and certain ORFs. Their sophisticated... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...changes were introduced to nCas9. These changes promote nCas9’s dissociation from DNA after nicking and... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...Expression is driven by a fairly strong EF1alpha promoter. We have verified expression of correctly-sized... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog Post... and funders in the Transparency and Openness Promotion Guidelines; preregistration to increase clarity...