CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
Type
Blog Post
... separately inducible by L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an...et al., 2015). pCRISPomyces-1 includes Cas9, a tracrRNA, and a CRISPR array, while pCRISPomyces-2 contains...presence of the inducer, in this case anhydro-tetracycline (aTc). By cloning in two tandem copies of the...