We narrowed to 474 results for: organization Addgene

...the membranes into stacked structures including organized smooth endoplasmic reticulum (OSER) whorls. 2)...distorted into dense stacked membranes termed Organized Smooth Endoplasmic Reticulum or OSER if a dimerizing...can be monomerized with an A206K mutation [2]. Addgene has many of these constructs and they are designated...PMCID: PMC3324619. Additional Resources on the Addgene Blog Read Professor Snapp's Post on mOX FPs Find...in 720°, i.e. they are on a membrane or in an organelle, then their effective concentration will be much...in Your Experiments Additional Resources on Addgene.org Browse our Fluorescent Protein Resources Find...

...Addgene's plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates...mTORC2 influences cell survival and cytoskeletal organization. Cancer Impact Enhanced signaling through either...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...BFP Ervin Welker Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...default backgrounds all year and want a change? Addgene has you covered. Today we’re releasing 11 bold ...reading this blog, chances are you are a fan of Addgene or our very cute mascots!  Blugene has been helping...might want to use one of our virus backgrounds. Addgene’s viral service provides ready-to-use AAV and lentiviral...the joke? If not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more ...background.     You can find all of the plasmids that Addgene offers by visiting our collections page. Here, ..., you can browse plasmids by field, organism, research technique, and more. Download the green DNA background...background.     Since we ❤️ DNA at Addgene, we're presenting you with another DNA background option, Download...

...the United States but around the world. As an organization that directly collaborates with NIH-funded researchers...utilizes grants to support our own initiatives, Addgene is deeply concerned about these developments. These... We Are Here to Help Despite these challenges, Addgene remains steadfast in our mission to accelerate ...vital during this period of constrained funding. Addgene is uniquely positioned to help research institutions...Universities and research institutions can turn to Addgene as a trusted partner for archiving their biological...scientific progress depends on our collective response. Addgene stands ready to collaborate with partners across...work and what support would be most valuable from Addgene during this time. Your input will directly shape...

...quietly learning about Addgene’s past and present, but the real promise of Addgene lies in our future. As...every aspect of our organization. One thing that will remain the same is Addgene’s commitment to supporting...Addgene saw big changes in 2022, from the launch of a new material to the redesign of a community resource...number of people. I look forward to working with Addgene colleagues, collaborators, partners, and the larger...

...entirely. Auxotrophic selection relies on the host organism being unable to synthesize a nutrient required... to design your vector to be functional in the organism your experiment takes place in. In many cases,...vectors a success!   Resources Resources on the Addgene blog Plasmids 101: Transformation, Transduction...Plasmids 101: The Promoter Region Resources on Addgene.org Collection of empty plasmid backbones ...

...generated using random genomic fragments from the organism of interest, or a defined cDNA library (e.g. the...number of two-hybrid systems available in model organisms, including bacteria, alternative fungi (C. albicans... Pubmed Central PMCID: PMC514463. Resources At Addgene Browse Our Yeast Expression Vectors Find Bacterial...

...specimens and for in vivo imaging of transparent organisms. Advances in tissue clearing Similar to light ...3DISCO (3-dimensional imaging of solvent cleared organs) method of imaging rodent brains and spinal cords...).  These viral plasmids are available through Addgene.  The combination of LSFM with multiple tissue ...

...your host organism. Because transcription machinery differs between cell types or organisms, promoters...writing of this article. Additional Resources on the Addgene Blog Browse All Plasmids 101 Posts Learn about...GFP Resources on Addgene.org Find Plasmids for Your Research     ...  Kendall Morgan...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...discussion, we bring up a performance Ben and Niroshi organized for Tyler's (the host's) thesis defense. This ...Additional Resources on the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research...Process  Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your...

...for Scientists Resources on Addgene.org Learn about Careers at Addgene ...accordingly. Read Additional Career Posts on the Addgene Blog How did this change in labor laws come about... salaries (which have been made by multiple organizations across academia). Increased postdoc wages, overtime... salaries as their website implies. These 5 organizations, according to the NSF data, account for 18% ...Research @FORsymp.   Additional Resources on the Addgene Blog Read about the 2015 and 2016 Future of Research...

...region (approximately 500bp is best) and select the organism. It will then tell you the available gRNA targets...can also find many previously validated gRNAs at Addgene. In vitro screening for the best gRNA Figure...Find Plasmids for Testing Your gRNAs in vitro at Addgene CRISPR/Cas9 gene editing in mice Now that you’ve... much more from our favourite mammalian model organisms in the near future.  Many thanks to our guest...PMCID: PMC3969854. Additional Resources on the Addgene Blog Get the Basics of Mouse Genome Engineering...Homology Directed Repair Additional Resources on Addgene.org Find Software Tools to Help Design You gRNA ...

...and stably maintain the genes they carry among organisms belonging to many different phylogenetic groups...bacteria and express proteins within different host organisms. pBBR1-based plasmids and plasmids in incompatibility...  Find broad host range recombinant vectors at Addgene!   References and resources References Doran, ...10.1007/s13205-023-03507-0  More resources on the Addgene blog Plasmids 101: Origin of Replication Plasmids...

...Blotting Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based technique, validation...you are confident, especially if you work in an organism that is underrepresented in the biomedical research...10.1038/nmeth.3995 Additional Resources on the Addgene blog Learn about research applications of antibodies...

...over-express coding sequences from a given cell type or organism, have been used in a similar manner to Cas9 activators...lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3) and organisms (Chavez et al., 2016). To learn more about the...    Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory at Columbia...doi.org/10.1038/nbt.3149 Additional Resources on the Addgene Blog Learn how to Choose the Best Cas9 Variant... CRISPR Has Been Used with AAV Resources on Addgene.org Find Cas9 Activators Find Cas9 Repressors Catch...

...plasmids with Addgene. Additional control plasmids for the TKO library will be available at Addgene soon. Hart...cellular compartments (cytosol, Addgene catalog #71245; mitochondria, Addgene catalog #71247) Rosella provides... in vitro cell systems and diverse model organisms. Addgene’s newest TALEN kit, the FusX assembly system...read? Listen to our hot plasmids segment on the Addgene Podcast. View Plasmids from:  March (Q1) | June...sequencing. The STARR-seq backbone is available from Addgene in both human and fly versions. Arnold, et al....alongside a growing number of CRISPR libraries from Addgene’s repository that can be used to perform screens...PMID: 24395366 Oxford Drosophila CRISPR library Addgene has had the privilege of distributing several human...

...  Additional resources on the Addgene blog Get started with Addgene's science career resources Browse...hub assembled a coalition of higher education organizations committed to advancing career and professional...

...plasmids you use to bring this into your target organism. We have designed a Golden Gate system based on...The complete GreenGate toolkit, now available at Addgene, also gives the option to stack several expression...motivations for making GreenGate available through Addgene,” Lohmann said. “We strongly believe the free sharing...  Kendall Morgan...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Along these lines, Darrell recently joined the Addgene Board of Directors where he hopes to help keep ...of this technique will someday allow us to make organs using iPS cells derived from patients. Chapter ...Chapter 12: Darrell's goals as a member of the Addgene board of directors - increasing open source sharing...Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids from the...