We narrowed to 801 results for: MAK
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TypeBlog Post...providing quality control and customer support, making plasmids easy for you to find and request in our... plasmids as of March 26, 2015. How does Addgene make it happen? Our awesome team of Addgenies! Our team...
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A Scientist's Guide to Ebola
TypeBlog Post...only viral protein present on the virion surface – making it critical for viral attachment and fusion. Once... The EBOV glycoprotein’s role in infection also makes it a critical component of vaccines and the target... -
Addgene's Website Update
TypeBlog Post...changes to our website! Part of sharing science and making access to materials easier is improving the tools...may be less obvious — but every change is meant to make your experience on our website better so you can... -
Microbes: Look, Listen, and Tell
TypeBlog Post...amoebasisters.tumblr.com/post/139944465237/lactose-around-we-can-make-some-enzymes-for The take home message was that... that being creative and telling a story makes scientific topics both interesting and approachable! ... -
Developing Transferable Skills During Science Training
TypeBlog Post...able to communicate effectively is part of what makes working in a team productive. Take all the opportunities...or want to start a peer mentoring group? "What Makes a Good Mentor?" Form Your Own Peer Mentoring Group... -
Why Do I Need an MTA Anyway?
TypeBlog Post... by the Provider Limited liability Addgene makes it easy Even with all those benefits, we know that...auto-approve MTAs. Whatever your preference, we are here to make it fast and easy. Fast Approval: The UBMTA used... -
Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog Post...in the process. “We had several motivations for making GreenGate available through Addgene,” Lohmann said...system, the more building blocks will be available, making it easier for us to do our work.” References 1... -
Let There Be LITE Plasmids
TypeBlog Post...Konermann said she is already aware of research groups making use of the new optogenetic tools in C. elegans ...neurons, she offers a tip: save yourself some time by making an AAV1 supernatant. (The details can be found ... -
Editor's Choice, September 2016
TypeBlog Post... your obscure growth medium, these apps can help make your lab life a bit easier. Since the post doesn...apps you’ve found useful in the comments section to make this post even more useful to your colleagues. Technique... -
Management for Scientists: Seeking Feedback
TypeBlog Post...eagerness to get, as well as give, information to make improvements. Members connect directly with one...card, afternoon off, etc.) Act on information to make change whenever possible. Be public about changes... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog Post...gRNAs can save you the time that would be spent making and testing entirely new gRNA designs. You can ...How to Design Your gRNA Use the CRISPR Software Matchmaker Browse Alll of Our CRISPR Posts Additional Resources... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog Post...and her lab members have noticed that the meetings make the lab run more smoothly. With a concrete plan ...Seeking Feedback Management for Scientists: What Makes a Good Manager Anyway Developing Transferable Skills... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog Post...the last 10 years as well as the many people that make up the Addgene family. It was great to see the faces...Just as her grandfather used the printing press to make copies of the newspaper, Addgene uses E. coli bacteria... -
Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog Post...servers and in journals, and researchers have been making new materials like plasmids available to scientists...as the best way that the scientific community can make progress towards understanding this disease. ... -
Working 9-5: an Addgene Co-op
TypeBlog Post... needed to go to an appointment at 3 PM, I could make up the hours I had missed at another time. This ...case for me at Addgene. There is a clear effort to make sure people feel connected. As I became accustomed... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog Post...look, and a reorganization of existing content to make it more accessible. Other accessibility updates ...typically adopted by for-profit companies, while making use of our nonprofit status to achieve mission-... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog Post...pSiM24, can also be used with biolistic methods, making it useful for engineering plants that are not susceptible...available pCAMBIA and pKYLX vectors, respectively, making it ideal for large gene transfer. The M24 promoter... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...Cas9’s ease of use and ability to label live cells make this system truly advantageous. This new technique...altered in disease states. dCas9 gets a colorful makeover Even without its nuclease activity, catalytically... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog Post...: if there is no place to upload a cover letter, make your cover letter the first page of your resume ... don't ask, you will never get the help that can make a difference. Having your resume forwarded personally... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...sought to make complex genome modifications in Drosophila using multiple recombinases. To make multiple... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post...there, but the panelists were interesting enough to make up for the somewhat naïve questions. During the ...coincidentally speak the same Polish sub-dialect making their Skype communications extra fun) who shared... -
London Calling - Addgene’s New Europe Office in London
TypeBlog Post...interacting with people and talking about science makes her the perfect Addgenie and she can’t wait to see...even visit your lab! We look forward to helping you make some serious discoveries. 10 Years of Plasmid... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog Post... with short generation times and high fecundity, make zebrafish ideal for high throughput studies of optogenetics...depends on these charged particles and whether they make the inside of the neuron more positive or more negative... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog Post....1038/s41586-019-1323-z Peters, J. E., Makarova, K. S., Shmakov, S., & Koonin, E. V. (2017). Recruitment... -
Antibodies 101: Affinity Tags
TypeBlog Post... a little thoughtful planning, affinity tags can make protein purification a cinch. Types of affinity ...that is unlikely to perturb protein function. This makes it amenable to being left on your protein of interest... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog Post...a means to gain employment, so it’s important to make sure a masters helps you get a job you want. But...bench. So if you enjoy wielding a pipette, it might make sense to earn a master’s instead of a PhD. Deciding... -
The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place
TypeBlog Post...in large supplementary data files, BioGRID ORCS makes it possible for researchers to query all public ...conditions. This increased ease of access not only makes CRISPR screen results available to the entire biomedical... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post...in healthy patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin...awareness of FOP. Please consider running with us or making a donation via the link below. Please continue ... -
Management for Scientists: Managing vs. Leading
TypeBlog Post...in doer mode even late in our careers. Managers make sure the work gets done Managers do this by removing...communication. A manager must both hire and fire to make sure the team is the best it can be. Managers must... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog Post... my way to the meeting, and its history of code making and code breaking is wonderfully analogous to genetic...thanks to all the Addgenies who worked so hard to make this poster, offered suggestions and tips, and helped... -
10 Ways to Share Your Science!
TypeBlog Post... to share your science 1) If you don’t already make it a habit to talk – really talk – about the research...science, they have good advice for anyone looking to make their science matter. If you aren’t ready for a ... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...scientists rarely stop to think about how much easier it makes our lives. Plasmid transformation into E. coli is... strains have natural antibiotic resistances, so make sure your plasmid and E. coli strain are compatible... -
Choosing a Good Mentor for Scientists
TypeBlog Post...serve as a good mentor. To read more about what makes a good mentor, see the previous post in this series...posts in our Science Mentors series: Part 1: "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog Post...was awed by the teamwork and initiative it took to make all this happen with 80% of the team working remotely...Protection Program loan and received enough funds to make most of our payroll for the spring and early summer... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...has developed a new CRISPR screening method that makes use of guide RNAs with internal barcodes (iBARs)... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog Post...Biotechnology that now can be deposited at Addgene. What makes these fluorescent proteins unique and what can they...excitation at 493 nm and max emission at 509 nm), making it useful in multicolor labeling particularly with... -
Summer SciComm Series: Master of SciComm
TypeBlog Post...whirlwind of skill acquisition. Classes covered filmmaking, podcasting, philosophy of science, creative ...misinformation in the wake of a global pandemic can make for a sore reminder of how far we have yet to go... -
Intro to Our Summer SciComm Series!
TypeBlog Post...science communication research. This summer, we’re making time to talk about my favorite thing - science ...but the foundation of all good communication is making sure everyone’s on the same page. So for our first... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog Post...stop for refreshment along the way? What ecosystem makes your heart sing with delight? From developmental...other scientists, or science journalists, or policy makers. The term editor covers a wide variety of careers... -
Plasmids 101: TOPO Cloning
TypeBlog Post...extra extension time after the last cycle of PCR to make sure that the "A" gets added to all PCR products... the standard ligation reaction is fairly quick, make sure you stay organized and prepare everything you...