We narrowed to 642 results for: Emb;
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TypeBlog Post...this surface”….where the complex is docked on the membrane of the endoplasmic reticulum. And graduate students...
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Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...principle, Peters and colleagues constructed a 40-member library targeting 20 genes of Enterobacter cloacae... -
Hot Plasmids: Summer 2025
TypeBlog Post...allows VAMP2-At to remain on the presynaptic cell membrane while AF, fused to a payload, is released into... -
Common Injection Routes in Mice
TypeBlog Post...When considering what method you’d like to use, remember that the final decision will likely come down ... -
Pooled Library Amplifications
TypeBlog Post... protocols attached. Final step: validation Remember, there is no “one-size-fits-all” protocol! Each... -
Antibodies 101: Flow Compensation
TypeBlog Post...But we hope this guide can help you get started. Remember, you can always change your compensation values... -
Of Myc and Men
TypeBlog Post...mapping functional domains of Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002/j.1460-2075.1984... -
Transferable Skills: Negotiation
TypeBlog Post...colleagues and supervisors and lead to you being remembered as a person who tries to find solutions that ... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...camouflage. An anemone’s red colored tentacles resemble worms which attract fish for them to prey upon... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog Post... We invite feedback and contributions from all members of the research community as we continue to to ... -
Experimenting with New Careers while in Grad School
TypeBlog Post...positive. I went back into lab the next day and remembered why I still love science. Keep building on your... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...provides useful information about how the vector was assembled, and enables requesting labs to utilize and modify... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post...the cell. Can be used for generating transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified... -
Viral Production
TypeCollection...filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, ... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog Post...commitments, respond to any issues raised by team members, and plan for the future of the team. All while... -
AAVs in Retinal Gene Therapy
TypeBlog Post...later, is it finally back in the limelight. On December 19, 2017 the FDA approved Luxturna™ the first ... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post... Science Night, a chance for parents and other members of the community to share their expertise with ... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post... materials expired? Did you pipette carefully? Remember to discard flow-through solutions and use clean... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog Post...system, cell lines typically used include Human Embryonic Kidney (HEK) cells and Chinese Hamster Ovary (... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post...’t work for all bacteria. Zeocin Zeocin is a member of the bleomycin family of antibiotics, which induce... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...out your Cas9? It can be! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a... -
Summer SciComm Series: Modes of Communication
TypeBlog Post... limiting factor at multiple points. Before you embark on a project, do your best to understand what kind... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...mammalian cells. Like Cas9 nucleases, Cpf1 family members contain a RuvC-like endonuclease domain, but they... -
Fluorescent Proteins: FRET
TypeCollection...Jin Zhang Lab at UCSD Content last reviewed: 25 September 2025... -
Lentivirus Plasmids
TypeCollection...pSLCAR-CD19-28z 3rd Modular CD28-CD3z CAR backbone. See Bloemberg et al. (2020) for additional CAR backbones. Scott... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...feeling weird and started to be fun. I just had to remember to approach the relationship with the perspective... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog Post...Remember the game show “The $25,000 Pyramid” where one player tries to get the other to guess a category... -
Transferable Skills Guide: Career Planning Resources
TypeBlog Post...clearly a good idea to be diligent in your research, remember that you will not be a graduate student or postdoc... -
Career Insights: Technical Support Specialist
TypeBlog Post...the larger biotech companies), and as far as I remember, it was the first time that I was absolutely convinced... -
Neuronal labeling with Spaghetti Monster
TypeBlog Post... Flying Spaghetti Monster; the smFP structure resembles images of the deity (see comparison image above... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...al. 2016. “Inhibitory Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo.” Neuron... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...noradrenergic excitability upon acute stress." The EMBO journal 37.21 (2018). PubMed PMID: 30209240. PubMed... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...working vector system This allows you to quickly assemble vectors and test different ideas. We are a huge... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog Post...identified. While LY6A or other Ly6 gene family members are linked to viral susceptibility (mouse adenovirus... -
Hot Plasmids Spring 2024
TypeBlog Post...nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative control... -
Viral Production at Addgene
TypeBlog Post...filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, ... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...sensitive to toxicity from RNA editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development... -
Antibodies 101: Immunoprecipitation
TypeBlog Post...adjusted depending on the sample. It is important to remember that sample lysates will also contain proteases... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...find tables and information on different viral membranes, tropism, and advantages and disadvantages: Table... -
Plasmids 101: Plasmid Incompatibility
TypeBlog Post...resistant plasmids from Enterobacteriaceae family members within the mouse gut (Kamruzzaman et al., 2017)... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post... on the expertise of the senior leadership and members of their consulting staff. Who would enjoy healthcare... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...., Rubinstein, M. “LDL receptor and its family members serve as the cellular receptors for vesicular stomatitis... -
Plasmids 101: Control Plasmids
TypeBlog Post...difference and is it significant? Vaux DL, et al. EMBO Rep. Apr; 13(4): 291–296. (2012). PubMed Central... -
Tips for Using FRET in Your Experiments
TypeBlog Post...Applications: An EAMNET Teaching Module http://www.embl.de/eamnet/downloads/modules/FRET_teaching_module.pdf... -
CRISPR Guide
TypeCollection...against these foreign nucleic acids. Acr family members inhibit CRISPR by a variety of mechanisms, including...Mediated Genetic Screening in Mice with Haploid Embryonic Stem Cells Carrying a Guide RNA Library. Cell ... -
CRISPR Plasmids - Tagging
TypeCollection... use in SapTrap reactions for targeting vector assembly, and the remaining 5 plasmids include Cre and ... -
Transferable Skills Guide: Time Management
TypeBlog Post...physically cross things out (it just feels good) and I remember things better when I write them down. Some people... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...excellent efficiency using a commercial isothermal assembly master mix. Introduction of the strong transcriptional... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...luck with any experiments involving IDLVs. And remember: You can never trust a lentivirus with secrets... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog Post...running your analysis. In this case, I and other lab members had worked with lymphocytes before, so I knew where...