We narrowed to 784 results for: SCR;

1. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

   Type

   Blog Post

   ...toolbox, Ed Boyden’s lab developed a way to rapidly screen thousands of proteins for one that can report electrical...

2. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

   Type

   Blog Post

   ...enzyme that controls apoptosis and regulates transcription. Without TKA1, cells proliferate and cannot ...

3. Human Germline Editing Using CRISPR

   Type

   Blog Post

   ...disorders, pre-implantation genetic diagnosis (PGD) screens embryos to find those that do not carry a given...

4. Antibodies 101: Conjugation

   Type

   Blog Post

   ...Stephenson W, et al (2017) Simultaneous epitope and transcriptome measurement in single cells. Nat Methods 14:...

5. Giving gRNAs a Facelift - Synthetic and Beyond

   Type

   Blog Post

   ...conformationally restricted RNA which improves mismatch discrimination and is more nuclease-resistant, resulting in...

6. Transferable Skills Guide: Cross-team Communication

   Type

   Blog Post

   ...when applicable Any other relevant information Prescribing an official name to all of our plates, tubes,...

7. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

   Type

   Blog Post

   ..., they are not always easy to track at the transcriptional or translational level. However, several genetic...

8. Progress Towards a PAM-Free CRISPR

   Type

   Blog Post

   ...Between SpRY and several of the other Cas variants described, nearly all genomic landscapes should be editable...

9. CRISPR in the Clinic

   Type

   Blog Post

   ...potential for treatment is no longer an accurate descriptor; progress in clinical therapy is more fitting...

10. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    ...populations of interest. Many of the calcium sensors described are also already packaged into viral vectors and...

11. High Complexity Golden Gate Assembly with NEB’s DAD

    Type

    Blog Post

    ...Additionally, this assembly, which used only antibiotic screening for selection, failed under normal cycling protocols...

12. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    ...involve needles or scalpels (sharps). Cuts and scrapes from sharp equipment are one of the most common...

13. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    ...ELISA is a modification of the sandwich ELISA as described here, the direct and indirect ELISA (more details...

14. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    ...to share or remix the songs, the CDs could get scratched over time, and it was difficult to keep track ...

15. Hot Plasmids - October 2020

    Type

    Blog Post

    ...Addgene The Cas Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express...

16. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type

    Blog Post

    ... I can have on an organization beyond my job description.” Our resident bagel provider, source of energy...

17. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    ...proteins in the lab, clinicians might consider prescribing GdnHCl to patients suffering from rare diseases...

18. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    ...20 additional days at which time the cells are scraped, and lysed by multiple freeze-thaw cycles. The ...

19. Rabies and Neuronal Tracing

    Type

    Blog Post

    ...widely used in neuroscience. In this post, I’ll describe how rabies virus (RABV) can be used in the brain...

20. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    ...corresponding S. cerevisiae met deficiencies (manuscript in preparation), indicating that Met protein ...

21. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    ...systems, this system incorporates the Tet-off transcriptional activation system to drive high levels of expression...

22. Science communication: 9 Strategies to Get Your Foot in the Door

    Type

    Blog Post

    ...now. These 9 strategies will get you started:  Subscribe to Career Blog Posts from Addgene 1) Twitter –...

23. 6 Tips for Grant Writing

    Type

    Blog Post

    ...are unlikely to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor...

24. Tips for Technical Support Calls

    Type

    Blog Post

    ...plasmids and pooled libraries were originally described in a peer-reviewed scientific publication. We ...

25. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    ...poster format differs in many ways from writing a manuscript. If you have ever attended a scientific conference...

26. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    ... can also deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are ...

27. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    ...for Cas9 (42 nt vs 100 nt.) However, the newly described non-CRISPR protein NgAgo is smaller than any of...

28. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    ...manual skills at the regular microscope, and the scripting skills at the automated microscope  to keep our...

29. How-to: 5 Steps to a Great Panel Discussion

    Type

    Blog Post

    ...organizations, I asked audience members to stand and describe unique nonprofit models that differed from the...

30. Plasmids 101: Degron Tags

    Type

    Blog Post

    ...proteasome for degradation. Like several other tags described here, the dTAG uses a small molecule, a tag, and...

31. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    ...signature upon antibody binding, leading to discrepancies during unmixing. Additionally, it is critical...

32. Of Myc and Men

    Type

    Blog Post

    ...that perfect. One of the known limitations was described by Schüchner, et al., who published a paper showing...

33. Hot Plasmids: Summer 2025

    Type

    Blog Post

    ... separately). Figure 5: Mechanism of ATLASCre. VAMP2 = synaptobrevin 2; At = ALFA-tag; BACE ...

34. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    ...whether or not newly synthesized or long-lived transcription factors control gross changes in gene expression...

35. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    ...better suited for genome-wide CRISPRi library screening experiments where maintaining library diversity...

36. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type

    Blog Post

    ...(the Gal4/UAS system), and the third post will describe how you can make your own mutant flies. Find Drosophila...

37. Twenty Years of Addgene Sharing: CRISPR

    Type

    Blog Post

    ...double-stranded break, while modulators regulate transcription and chimeric efforts make a precise edit or ...

38. PhD Applications After COVID

    Type

    Blog Post

    ...lab, research, and provide them with a CV or transcripts if requested Thank them, and eagerly await a ...

39. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    ...Center are now available at Addgene along with a description of their experimental protocol. The three plasmids...

40. Plasmids 101: Optimizing Plasmid Yields

    Type

    Blog Post

    ...concentration Low copy plasmids produce fewer transcripts of their antibiotic resistance genes and can ...

41. PITChing MMEJ as an Alternative Route for Gene Editing

    Type

    Blog Post

    ...Building on the lab’s previous work, Sakuma et al. describe a detailed protocol for MMEJ-mediated knock-in...

42. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    ...Antibiotic Resistance Plasmids 101: Blue-White Screening Choosing Your Perfect Plasmid Backbone  ...

43. An Introduction to Adenovirus

    Type

    Blog Post

    ... (and therefore between early and late gene transcription) is demarcated by viral genome replication (...

44. A Guide to Starting Your Own Journal Club

    Type

    Blog Post

    ...served. I hope that with some of the information described above you feel empowered to start your own journal...

45. SciComm: Taking Science to Elementary and Middle Schools

    Type

    Blog Post

    ... itself in terms of what they do with their discretionary money. They also usually have a good event-planning...

46. Tips for arabidopsis transformation

    Type

    Blog Post

    ...generate transgenic Arabidopsis, from studying transcriptional and translational dynamics of genes and proteins...

47. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    ... minimize false positive colonies. We routinely screen just two colonies for simple cloning procedures...

48. CRISPR 101: Making a Knock-In Cell Line

    Type

    Blog Post

    ...reading up on validating genome edits to find screening strategies for your experiment. Also learn about...

49. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    ...Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically or by PCR/sequencing ...

50. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    ...side of things from taking too much of your time, scroll down for some tips on how to make a successful ...