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  1. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...species of yeast can be used for protein expression, S. cerevisiae, is the most reliable and frequently used...organism in genetics and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins...
  2. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical...-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary Antibodies...
  3. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...by secondary structure. It is thought that poly(A)s are used to control the cellular concentration of ... end formation. Cell. 1987. PMID: 3568131.  Hager S, Frame FM, Collins AT, Burns JE, Maitland NJ. An Internal...
  4. Lentivirus ddPCR Titration

    Type
    Protocol
    ...dilutions and titrations Reference Wang Y, Bergelson S, Feschenko M. Determination of Lentiviral Infectious...
  5. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...dentate gyrus and olfactory bulb. Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):E1152-61 Pubmed PMID 23487772...73. Pubmed PMID 22681690.  8, Wickersham IR, Finke S, Conzelmann KK, Callaway EM. Retrograde neuronal tracing...
  6. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...targeted sequence at the locus of interest. SpCas9’s 3’ NGG PAM occurs frequently in GC-rich genomes, but...AT-rich loci or genomes. Cas9s from other species, like S. aureus and N. meningitidis require different PAM ...
  7. Common Injection Routes in Mice

    Type
    Blog Post
    ... https://doi.org/10.4236/jbm.2020.89011 Shimizu, S. S. (2004). Routes of administration. In H. Hedrich...
  8. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...components, and the accessibility of your target epitope(s). To achieve these goals, researchers commonly use...//doi.org/10.1177/33.8.3894499    Im K, Mareninov S, Diaz MFP, Yong WH (2018) An Introduction to Performing...
  9. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...discussing recent publications in a specific field(s). Most anyone who has spent any time in academia has...of critique and detail? Who is/are the facilitator(s) of the journal club? Where will the journal club ...
  10. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...TALENs and CRISPR. MMEJ is active during M and early S phases, when HDR is inactive, and the balance of NHEJ...still being explored. More broadly, Sakuma et al.’s use of MMEJ represents another strategy researchers...
  11. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...difference! Find mGold2 plasmids here! Lee, J., Lai, S., et al. (2025). Bright and photostable yellow fluorescent...here! Singh, I., Fernandez-Perez, D., Sanchez, P. S., & Rodriguez-Fraticelli, A. E. (2025). Pre-existing...
  12. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... Jul 27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an exceptional genome... this paper at Addgene.  3. Gootenberg, Jonathan S., et al. "Nucleic acid detection with CRISPR-Cas13a...
  13. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ... the genome to introduce the genetic modification(s) contained within it by selecting for Zeocin resistance...19416369. Kulakauskas, Saulius, Arvydas Lubys, and S. Dusko Ehrlich. "DNA restriction-modification systems...
  14. Tips for arabidopsis transformation

    Type
    Blog Post
    ...transgenic plants in the process.     References 1. Emami, S., Yee, M. and Dinneny, J. R. (2013) ‘A robust family...24032037. PubMed Central PMCID: PMC3759027. 2 Harrison, S. J. et al. (2006) ‘A rapid and robust method of identifying...
Showing: 221 - 240 of 430 results