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Showing: 221 - 240 of 391 results
  1. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...systems, expression of S. pyogenes Cas9 or Cas9 variants (hereafter referred to as Cas9) and a single stranded... the nucleus, and several codon optimized Cas9 variants have been created in an effort to increase translation...
  2. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Tet-Off vectors (Tet-Advanced)” are the most recent variants of the Tet-Off system.  For more information on...pADH1 activity. Researchers have engineered pADH1 variants that are less sensitive to ethanol, including ...
  3. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...mutated genes. Next, the authors explored how these variants of p53 shape chemosensitivity and mechanistic ...provides a HiBiT-enabled framework for quantifying variant expression with a minimally disruptive tag. Find...
  4. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...integrations per cell, and my stable cell pool had wildly variable Cas9 expression (Figure 1). This is a problem ...monoclonal lines (Figure 2) and I was surprised at how variable Cas9-expression was across the different cells...
  5. Luciferase Plasmid Collection

    Type
    Collection
    ...Highlighted Tools Modified/Novel Luciferases Engineered variants of luciferases with altered emission, substrate...
  6. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...HaloTag is actually HaloTag7, and recent specialized variants run up to HaloTag11. HaloTag is now the most popular...any cloning at all! As mentioned above, several variants of these tags have been developed over the years...
  7. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ... loop to produce SuperFi-Cas9, a high-fidelity variant that still retains high cleavage efficiency (Bravo...Components of CRISPR  xCas9: Engineering a CRISPR Variant with PAM Flexibility Cas9 vs the Other Cases Further...
  8. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...et al. A percentage is not listed because many variables (cell type, criteria used, etc.) can effect reprogramming...insertional mutagenesis and random integration creates variation between iPSC clones. Retroviruses are not considered...
  9. Plan Your Experiment

    Type
    Collection
    ... window from the PAM sequence, and base editor variants often have different optimal editing windows. ...
  10. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ... CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications...
Showing: 221 - 240 of 391 results