We narrowed to 564 results for: BLI
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TypeBlog Post...tested for antigen binding. If starting from an established hybridoma line, the antibody can be converted...Edition. 2104. Cold Spring Harbor Laboratory Publications, New York. Helma J, Cardoso MC, Muyldermans ...
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Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...they are making fairly remarkable progress on establishing a system for the chemical replication of RNA...created a circRNA website so scientists can view public circRNA datasets and download the custom python... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post...recently there has been a counter movement by the public to access basic equipment and to become involved...first hackerspace which now offers members and the public cheap access to personal manufacturing tools – ... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog Post...This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates...these people and bios were likely part of the publicity. Good panels do not waste time on long introductions... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post...poster board well ahead of time ensures you aren’t scrambling to tack up your poster seconds before the session...about the relationship of your work to recently published papers or “hot topics,” so be sure to be well-... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...constant, with fitness effects more pronounced at 12 doublings than at 6. Key takeaways of Mobile-CRISPRi Mobile-CRISPRi...PubMed PMID: 30675034. Peters, Jason M., et al. "Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...Originally published Sep 30, 2014 and last updated Dec 10, 2020 by Benoit Giquel. Adenoviral vectors...Manuel Gonçalves' lab found in a second paper published in Nature Methods the same year that delivering... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...Originally published Dec 7, 2017 and updated Jul 2, 2020. Promoters may be the star of gene regulation...Elements) method. The original CAPTURE method was published in 2017 and addressed many of the drawbacks of... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post...vectors? Viral genome sequencing Thanks to the establishment of in-house sequencing capabilities and Seqwell...our pipeline (see Figure 2, sample 2). When we blindly analyzed AAV-68544, we were easily able to identify... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post... into Addgene’s CRISPR data? Read our recent publication, “The Expanding Dissemination and Distribution...support for, this and previous Addgene CRISPR publications. References and resources References Pyhtila... -
Of Myc and Men
TypeBlog Post...well defined peptides. Munro and Pelham’s first published peptide tagging experiment involved a peptide ...limitations was described by Schüchner, et al., who published a paper showing that the amino acids neighboring... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an...or trying to explain your science to the general public, visit the Center for BioMolecular Modeling web... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog Post...identify your transferable skills? PLoS One recently published an article identifying gaps in scientists' transferrable...Collaborating with others outside of your organization Public Speaking Time management Managing a team Conflict... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major ...for RNA editing. Two years after that, the lab published a paper on an RNA editor that allows C to U edits... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...subfields. Plasmid requests correlate with paper publication The dataset includes 11,426 plasmid requests ...14%). Spikes in plasmid requests correspond to publications of popular papers. Figure 1: Plasmid requests... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post...classic, net-neutral dye scaffold due to its established chemistry, small size, brightness, and cell permeability...In past decades, further refinements to this established dye structure have yielded commercial panels ... -
CRISPR Antimicrobials
TypeBlog Post...suppliers, and public health and medical organizations are working to help educate the public about proper... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post...situation. So, young researchers need to publish good publications in a short period of time and may need... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post...This article was originally published on the Society for Laboratory Automation and Screening (SLAS) Electronic...can make a decent meal, fold their laundry, take public transportation, call a cab and remember their own... -
Neuronal labeling with Spaghetti Monster
TypeBlog Post...nervous system (CNS) orchestrates complex processes enabling organisms to control their movements and behavior...opposition to the teaching of intelligent design in public schools and was started in 2005 in Kansas by Bobby...