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  1. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...recently there has been a counter movement by the public to access basic equipment and to become involved...first hackerspace which now offers members and the public cheap access to personal manufacturing tools – ...
  2. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates...these people and bios were likely part of the publicity. Good panels do not waste time on long introductions...
  3. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Browse CRISPR Plasmids By Function Genome Editing...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...Plasmid Gene/Insert Promoter Selectable Marker Publication Prime Edit Cas9 H840A nickase fused to a reverse...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Activate Catalytically dead dCas9 fused to a ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Interfere Catalytically dead dCas9, or dCas9 ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Epigenetics To make targeted epigenetic modifications... ID Plasmid Gene/Insert Selectable Marker PI Publication RNA Knockdown Type VI CRISPR systems, including...
  4. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an...or trying to explain your science to the general public, visit the Center for BioMolecular Modeling web...
  5. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ... into Addgene’s CRISPR data? Read our recent publication, “The Expanding Dissemination and Distribution...support for, this and previous Addgene CRISPR publications.  References and resources References Pyhtila...
  6. Of Myc and Men

    Type
    Blog Post
    ...well defined peptides. Munro and Pelham’s first published peptide tagging experiment involved a peptide ...limitations was described by Schüchner, et al., who published a paper showing that the amino acids neighboring...
  7. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...constant, with fitness effects more pronounced at 12 doublings than at 6. Key takeaways of Mobile-CRISPRi Mobile-CRISPRi...PubMed PMID: 30675034. Peters, Jason M., et al. "Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi...
  8. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...poster board well ahead of time ensures you aren’t scrambling to tack up your poster seconds before the session...about the relationship of your work to recently published papers or “hot topics,” so be sure to be well-...
  9. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...vectors? Viral genome sequencing Thanks to the establishment of in-house sequencing capabilities and Seqwell...our pipeline (see Figure 2, sample 2). When we blindly analyzed AAV-68544, we were easily able to identify...
  10. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert Promoter PI Publication Drosophila ID Plasmid...Marker PI Publication Plant ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication C. elegans...Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Zebrafish...Marker PI Publication Xenopus ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Parasites...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that...
  11. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...identify your transferable skills? PLoS One recently published an article identifying gaps in scientists' transferrable...Collaborating with others outside of your organization Public Speaking Time management Managing a team Conflict...
  12. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major ...for RNA editing. Two years after that, the lab published a paper on an RNA editor that allows C to U edits...
  13. CRISPR Antimicrobials

    Type
    Blog Post
    ...suppliers, and public health and medical organizations are working to help educate the public about proper...
  14. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...This article was originally published on the Society for Laboratory Automation and Screening (SLAS) Electronic...can make a decent meal, fold their laundry, take public transportation, call a cab and remember their own...
Showing: 221 - 240 of 562 results