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We narrowed to 648 results for: CHI

Showing: 221 - 240 of 648 results

  1. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...instability-prone lentiviral vector plasmid in Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org...lentiviral expression vectors using reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org...cloning strategy for efficient genome editing in Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186...

  2. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...including a mouse monoclonal (M2) as well as a chimera (rabbit). Myc The Myc tag is a 10 amino acid peptide...still the most prevalent clone used today! Goat, chicken, mouse, or rabbit? Addgene has you covered with...Addgene’s monoclonal antibodies (mouse, rabbit, and chicken) to this tag! GFP  GFP is more than a peptide –...

  5. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...guides – including an excellent series of posts by Joachim Goedhart on the Addgene blog – outlining various...  Simply input these parameters and you’ll find matching FP suggestions. If you’re not sure exactly what...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

  6. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...their structure or their interaction with AAVs (Schiller et al., 2018). Exosomal vesicles secreted from...behind these improvements. What have exosome AAVs achieved so far? Exo-AAVs have improved transduction profiles...PMID: 29296848. PubMed Central PMCID: PMC5728288. Schiller, Lara Timantra, et al. "Enhanced Production of...

  7. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ... our purposes? In search of simple biological machines R bodies can repeatedly extend and contract...using R bodies: simple, force-generating protein machines that can extend and retract over many microns ...with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed us to ...

  8. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...Refactoring and optimization of light-switchable Escherichia coli two-component systems. Schmidl SR, Sheth... at Addgene. Synthetic biology: engineering Escherichia coli to see light. Levskaya A, Chevalier AA, ...Multichromatic control of gene expression in Escherichia coli. Tabor JJ, Levskaya A, Voigt CA. J Mol Biol...

  10. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...can be affected by both autofluorescence and photobleaching. Researchers have long been interested in using... cells. As Nano-lanterns are not subject to photobleaching/phototoxicity, they successfully imaged multiple...applications. For long-term imaging, especially where photobleaching and phototoxicity are a concern, these constructs...

  11. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...instead use a preexisting, conserved cellular machinery to process gRNAs. Hijacking tRNA processing to...plants. Yang was intrigued by the tRNA processing machinery present in almost every living organism. Precursor...PTG constructs in rice protoplasts. Compared to matching traditional gRNA-expressing constructs, transcript...

  13. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...GFP. The lab tested the base editors in vivo and achieved therapeutically relevant editing efficiencies ...heart, and skeletal muscle. The dosing required to achieve these efficiencies was also between 1013 - 1014...Ace absorbance increases and results in higher quenching of mScarlet emission. Figure 2: Ace-mScarlet...

  14. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...what Shoukhrat Mitalipov and his colleagues have achieved, and how the field will move forward from this...circumvent this problem, they co-injected CRISPR machinery with sperm into M phase oocytes, thus allowing...’s important to remember that the study hasn’t achieved any end goal not available with current techniques...

  15. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...developing assays.  Protocol architecture The basic (very basic!) architecture of the indirect approach is...method relies on multiple secondary antibodies attaching to a single primary antibody, it is sometimes ...

  16. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...back into the infants. Almost all patients have achieved persistent immunological reconstitution with a...mutations in the gene RPE65. It is characterized by childhood onset of blindness and was thought to be untreatable...Improvement and decline in vision with gene therapy in childhood blindness." New England Journal of Medicine 372.20...

  17. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...Intermolecular FRET can be experimentally difficult to achieve, because the ratio of acceptor to donor fusions... Before constructing your own FRET probe, try searching PubMed for articles describing the FRET tool that...PMC2396198. 4. Sustarsic, Marko, and Kapanidis, Achillefs N. "Taking the ruler to the jungle: Single-molecule...

  18. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ..., Jiang F, Xie J, Kamiyama D. Commun Biol. 2021 Daichi Kamiyama Cerulean Cerulean(1-10) for use with..., Jiang F, Xie J, Kamiyama D. Commun Biol. 2021 Daichi Kamiyama ECFP C155 of ECFP pairs with N173 derived..., Jiang F, Xie J, Kamiyama D. Commun Biol. 2021 Daichi Kamiyama sfCherry2 sfCherry2(1-10) and sfCherry2...

  19. A Look at Addgene's QC Process

    Type
    Blog Post
    ...intended. Sharing unverified plasmids can lead to far reaching consequences, from wasting time and money on optimizing...through the QC pipeline to resolve any issues.  Catching and addressing QC issues Addgene’s QC team has...discrepancies can be caught prior to distribution. Catching these errors prevents future experimental issues...

  20. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...multiple genes to achieve a desired effect. This is commonly used in plant biology to achieve traits such as...organization schema MoClo can be broken down into three hierarchical levels: individual DNA parts, transcriptional...
Showing: 221 - 240 of 648 results