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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 314 results for: Cas9

Showing: 221 - 240 of 314 results

  2. Validated gRNA Sequences

    Type
    Collection
    ...single species or PAM binding variant of Cas9 (e.g. Wild-type SpCas9 must be used with targets that are upstream...activity. Know your target. Which species or variant of Cas9 ( S. pyogenes, S. aureus etc.) was this gRNA sequence...and Resources page have been used to indicate the Cas9 application the gRNA was designed to accomplish....Target Species Target Sequence Plasmid ID Application Cas9 Species PubMed ID Depositor OCT4 H. sapiens CTCCCATGCATTCAAACTG...10 C. elegans CGCTACCATAGGCACCACG 71516 cut VRER Cas9 mutant 26680661 Fire dpy-10 C.elegans GCTACCATAGGCACCACGAG...-10 C. elegans TCCGCTACCATAGGCACCA 71479 cut VQR Cas9 variant 26680661 Fire dpy‐10 C. elegans GCTACCATAGGCACCACGAG...Christiaen EGFP A. victoria multiple, see article 60071 dCas9-FokI S. pyogenes 24770325 Joung EGFP A. victoria...

  3. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...inserted the deaminase into the C-terminal domain of Cas9, which narrowed the editing window and reduced A...site. Like in most other base editor designs, the Cas9 nicks the non-edited strand to encourage the cell.... gGBE consists only of engineered MPG fused to nCas9. First, MPG removes the G base from DNA to create...

  5. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ... last few years, scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a, to develop ... variant that can also cleave dsDNA similarly to Cas9 (Zetsche et al., 2015 ). Cas12a however recognizes...

  6. Editor's Choice, August 2016

    Type
    Blog Post
    ...Viral Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect...uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at...

  8. Antibodies 101: Validation

    Type
    Blog Post
    ...to knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate expression...gene for your protein has been mutated using CRISPR/Cas9. You check the mutation and it causes a large deletion...

  10. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...activity and minimize off-target effects of CRISPR-Cas9." Nature biotechnology 34.2 (2016): 184-191. PubMed...PMC5445660. 7. Chari, Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library...used for each gene. At the moment, 13 full genome SpCas9 knockout libraries designed by different academic...Library, for example related to strategies beyond SpCas9, or species not currently included in Green Listed...libraries. As of now I have focused on human and mouse SpCas9 knockout libraries, but there are of course great...

  11. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ... for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin...inactive dCas9 is used to purify genomic DNA bound by the gRNA. An epitope tag(s) can be fused to dCas9 or ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system. The ...purification, design your gRNA sequence to direct dCas9 to a specific locus, avoiding known transcription...

  12. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require that each DNA target have...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  13. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ... inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9 into a customizable...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  14. CRISPR Plasmids - Parasites

    Type
    Collection
    ...research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and forward...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some...

  16. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ...lentiviral plasmids, catalytically inactive Cas9 (dSpyCas9) fused to APEX2 is expressed from a Tet/Shield1...genomic loci. He made use of catalytically inactive dCas9 by fusing it to an engineered peroxidase, (APEX)...are enriched in the 20 nm radius surrounding the dSpyCas9-APEX2 fusion.  This Sontheimer lab used this technology... technology, which they call C-BERST (dCas9–APEX2 biotinylation at genomic elements by restricted spatial...

  18. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ... editing - all influenced by an understanding of Cas9 biophysics! Casilio: An Adaptive, Multitasking “... system that uses modular RNA binding proteins, dCas9, and a modified gRNA to direct a variety of customizable...
Showing: 221 - 240 of 314 results