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We narrowed to 570 results for: Cit

Showing: 221 - 240 of 570 results

  1. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...severing and has very low dark-state activity and toxicity. All of that means this light-powered sword truly... 30x SuperPLDs avoid dark-state activity and cytotoxicity better than the 50x and 100x variants. But those...great for in vitro biocatalytic applications where toxicity doesn’t matter. Plus, for folks interested in ...

  2. March for Science

    Type
    Blog Post
    ...government can be defined as a social contract citizens enter into in order to protect their rights (2...and often funds [3]) scientists as well as other citizens. Thus civilization proceeds in an informed fashion...political action, and inspires politicians and citizens to include science in decision making, it will...

  3. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...make it a great viral vector, but its packaging capacity is limited to ~4.7kb, or roughly half the packaging...Expressing Transgenes That Exceed the Packaging Capacity of Adeno-Associated Virus Capsids. Human Gene ...Expands Adeno-associated Viral Vector Packaging Capacity in a Transgene-independent Manner. Molecular Therapy...

  4. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... and note how they build suspense. Notice the simplicity of their slides and how the slides complement...about how the sharing model works. See if you can elicit helpful comments from your audience as in “Does...delivery, rapport with the audience, and their own excitement about the topic. Don’t be fooled by thinking ...

  6. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...popular due to its durability and high binding capacity. In addition, PVDF membranes can be repeatedly...times. If the vendor includes publications that cited the antibody, consider reviewing the materials and...with antisera: a method for studying antibody specificity and antigen structure. Proceedings of the National...

  7. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ... Cas9 expressing lentivirus at the indicated multiplicity of infection (MOI). Anti-vinculin Western blotting...shifting landscape of research opportunities, it’s an exciting time to be in the lab. Based on the power of available...easy it is to share information and regents, it’s exciting to think that no matter who you are, you could...

  8. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...after cleavage by the transposase. It has a cargo capacity >100 kB, although integration efficiency decreases...without decreased efficiency, with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac, Tol2 also...Nature Education 1(1):204. https://www.nature.com/scitable/topicpage/transposons-the-jumping-genes-518/   ...

  9. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...read many base pairs simultaneously to increase specificity. This modular approach made ZFNs highly appealing...individual nucleotides. This imparts TALENs more specificity and a broader substrate preference than the ZFNs...Seligman LM (2002) Mutations altering the cleavage specificity of a homing endonuclease. Nucleic Acids Research...

  10. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ...your experiment. They come in all sizes and specificities, from target-specific ones to application-specific...Search engine May include validation data CiteAb Any Any Search engine May include validation...

  11. Summer Fun at Addgene!

    Type
    Blog Post
    ...want to hear and see even more of us? We’re very excited to be launching the official Addgene TikTok at ...of shenanigans we get into!  We hope you are as excited as we are! Come join us in our blog, video, and...

  12. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...days away and here at Addgene we couldn’t be more excited! As a small, tight-knit organization we thrive ...the coming days. Hopefully, I’ve made clear how excited we get for Halloween and how seriously we take ...

  13. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    ...hours? A colossal 547 years! While we are very excited about reaching this milestone, it wouldn’t have...their contributions on social media as well the excitement amongst our Addgenies on social media or around...

  15. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...Lianna Swanson was very popular, with the students excited to see a real working lab full of busy Addgenies...toothpick was very cool!” and Izzy Shaw, who was excited about “Seeing a real lab!” And several of our visitors...

  16. CRISPR Guide

    Type
    Guide
    ... every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most...targeting multiple genomic locations or enhancing specificity, efficiency, or flexibility of the Cas enzyme...Vectors Engineering the Cas9 Endonuclease CRISPR specificity is determined by both the gRNA sequence and the... design, engineered Cas9s can enhance CRISPR specificity. SpCas9 (from Streptococcus pyogenes ) is the...many modified versions engineered to increase specificity and nuclease activity available. Engineered Cas9s...cleavage of target DNA. Cas9n increases target specificity. Since two nickases targeting opposite DNA strands...fidelity of Cas9 Researchers have also enhanced specificity by engineering Cas9 into high fidelity Cas9s ...

  19. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ... Dr. Mikhail Shapiro from Caltech presented an exciting way of listening to microbes by using non-invasive... sound to visualize bacteria in the lab, I was excited to learn about creative approaches to visualize...
Showing: 221 - 240 of 570 results