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We narrowed to 824 results for: EED

Showing: 221 - 240 of 824 results

  1. With Our New Viral Service, We're Taking CRISPR Further!

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    Blog Post
    ... studying. You need to make sure your assay is scalable and robust. What you don't need to do is spend... is to quickly generate a single cell line and proceed to use it for experiments.” - Alex Chavez, researcher...available as lentivirus and we’ll be adding more as the need arises. For example, we’ve teamed up with the Broad...When you're setting up to do a genetic screen, you need to focus on building a really good model that reflects...

  2. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...also use these tips as a starting point when you need to amplify a library for your own experiments. ...individual plasmid. However, lots of library DNA is needed for screening experiments, so the scale of the ...for most libraries. Here are the key points you’ll need to remember when undertaking library amplification... are identical except for the insert; there’s no need to sequence other parts of the plasmid. How will...reads is, again, seemingly straightforward – you need to count how many times each of the library components...

  4. How Addgene Creates Content

    Type
    Blog Post
    ...educational content Addgene truly believes that sharing speeds science, and our entire mission is to enable that...our frequently asked questions!    Identify needed content Let’s take a look at how we create this...first step was to understand information our guide needed to have. Through a series of conversations on resources...basic information someone new to antibodies might need to successfully start using them at the bench, along...in which they have expertise. Other times, we may need to reach out to an external expert to help us create...

  5. 3 Challenges in Plant Synthetic Biology

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    Blog Post
    ...provide a much needed boost to basic molecular plant research, more initiatives are needed to increase the...and opportunities abound to build better fuels, feeds, foods, and fibers. And because working with plants...that the scientific and the agricultural community needs to reclaim the term “GM” by demonstrating the current...the day cannot be developed through conventional breeding; synthetic biology is required to achieve this...

  6. Summer SciComm: Preprints

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    Blog Post
    ...and they provide different amounts of access or feedback. One of the most significant shifts in scientific...of strategies for your scientific communication needs. Going to present some unpublished results at a ...preprints as part of their scientific communication needs. The most often-cited reasons researchers might ... in most review processes. Preprints and their feedback, in contrast, are immediately and openly accessible...formal peer review, with the earlier attention and feedback giving authors the opportunity to strengthen the...

  7. Antibodies 101: Selecting the Right Antibody

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    Blog Post
    ...secondary should be used when signal amplification is needed.  Species  Next, check the species that the antibody...a good chance that the antibody could meet your needs.    Multiplex experiments Finally, consider whether... antibodies in a single experiment then you will need to make sure that you can easily distinguish each...purchase but also someone to turn to for advice if you need to troubleshoot. What do you know about the vendor...validation does not mean that the antibody is guaranteed to work. Antibody-based assays are complex and...

  8. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...technique, and sometimes each individual assay, will need to be developed and optimized for the samples, antibodies...multiple proteins of interest - the secondary antibody needs careful consideration before selection. If all the...precious commodity in the lab and eliminating the need for secondary antibodies would save a good deal ... antibody labelled with a probe, eliminating the need for a secondary antibody. Other immunohistochemistry...option for signal amplification while providing needed flexibility in assay development. Researchers who...

  9. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    .... 2. Studying negative feedback loops of signaling pathways Negative feedback loops are a common feature...kinase activity. Zhou et al studied the negative feedback on endogenous MEK1/2 phosphorylation by creating...PP1/PP2A is responsible for this MEK1/2 negative feedback. 3. Studying signaling pathways in vivo Expressing...possibly be used in other model systems, but they need to be expressed in a tissue or organism that blue...

  10. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...interpretations of what we discussed and decided. I needed to a) figure out the sources of miscommunication...almost certainly experienced some of the hurdles I needed to overcome.  I eventually pinpointed and (mostly... software engineers in your lab, you likely will need to write protocols, SOPs, and methods sections. ... not have the context you do, which means you’ll need to provide that context yourself. Simple words ... commonly used (and misused) words that clearly needed an Addgene-specific definition. Words such as “...

  11. Gendered Innovations: Why Does Sex of the Cell Matter?

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    Blog Post
    ...Researchers need to learn sophisticated methods of sex and gender analysis. Universities need to incorporate...research that fails less and helps more people, we need to take sex into account. Gendered Innovations is...Especially in animal research, they worry about needing more study subjects and control groups. The important...these methods into their curricula. Corporations need to integrate these insights into product design....

  12. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...finding a good one that is a style match for your needs can take some research.  It helps to spend some ...Take advantage of this to get the information you need to choose well. What are their qualifications? ...can't tell you which one will be a match for your needs, but here are a number I have come across for you...is Bridging Science and Business. Maybe you just need help getting through your PhD? Some coaches focus...matching with the best person in the group for your needs. Beth Schachter of Beth Schachter Consulting is ...

  13. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...variety of different career paths that don’t all need another degree, he advised me, “a master’s degree... where you want to go in your career you may not need those doors to be opened.” Addgenie Eric Perkins...assistant, when her life changed––she had a daughter. Needing a career switch because of the demanding hours ... the bench, the change gave her the balance she needed. It is incredibly helpful to me to know that you...like being with the people and for the people, feeding the people, and making them get outside of their...

  14. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ... Sánchez lab protocol, dsRNAs are delivered by feeding them to planarians. Depending on which planarian...and mix the purified dsRNA with liver paste, and feed the mixture to the worms. The phenotypes of RNAi...treatment should be apparent after three dsRNA feedings. Some of the phenotypes are not evident until ...published protocols and want to share pictures, feedback etc. please write to [email protected] ...RNA inhibits gene expression in planarians." Proceedings of the National Academy of Sciences 100.suppl...

  15. High Complexity Golden Gate Assembly with NEB’s DAD

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    Blog Post
    ...rules didn’t need to be strictly followed in order to generate high-fidelity reactions. (Need a refresher...redesign individual pieces as needed to improve fidelity. NEBridge GetSet Need a set of overhangs, stat? ... to 6-8 overhangs, the data-driven approach was needed to massively expand complexity. The three tools...

  16. Progress Towards a PAM-Free CRISPR

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    Blog Post
    ... where you need it, the odds of this occurring are not in your favor. Thus, there is a need to eliminate...correction of disease-causing alleles, and the growing need for gene editing in research, the old PAM constraints...advantageous in today’s genome editing landscape. The need for PAM-less editing   Guides for CRISPR are typically...you are trying to knock-in a patient mutation or need to make a precise amino acid change, there is very...

  17. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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    Blog Post
    ...student, she had less pressure to publish and more freedom to attend conferences, but after receiving her ...money is a drop in the bucket against the funds needed to build a flower that changes color, but the experience... us the chance to develop the technology we will need, and the business training to make the most of it... to do. It’s exhilarating to have that sort of freedom. It can also be overwhelming – there is so much...partners in this accelerator program who want us to succeed. We’re excited to see what the next few months ...

  18. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post
    ...important factors to consider for any experiment are: Speed Cost Difficulty Efficiency For cloning in ...Additional Resources” section at the end of the post! Speed: Your grant deadline is approaching or your advisor...also be a great choice, because the T4 polymerase needed for this method is relatively inexpensive and can...generating the appropriate inserts by PCR, there is no need for restriction digests, gel purifications, or ligations... involved with cloning, you would at least be guaranteed a successful result? While there are never any...

  19. Tips for a 1st time AAV user (by a Rookie AAV user)

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    Blog Post
    ...while also summarizing the basic steps and analyses needed for packaging AAV. AAV production Overview: The...HEK293 cells with AAV packaging plasmids. The cells need three different plasmids to produce AAV: 1) the ...this process takes 4-7 days, not counting the time needed to expand the HEK293 cells. Check out the Addgene...experiments, so I always make more AAV than I think I need. Unlike lentivirus, AAV requires a higher number...phases mixed together. Lastly, the PEG solution needs to be sterilized, either by filtration, which takes...

  20. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...will also need to deliver a donor plasmid or single-stranded DNA oligonucleotide, so you will need to make...with any experiment, there are many factors that need to be considered during the planning process. For...appropriate expression system and delivery method: Do you need stable integration or is transient expression sufficient...steps is to identify what CRISPR components you will need to deliver. Minimally, one or more sgRNAs and Cas9...
Showing: 221 - 240 of 824 results