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Showing: 221 - 240 of 711 results

  1. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...(FLAG, Myc, etc.) and fluorescent protein tags (GFP, mCherry, etc.). Fluorescent tags are primarily used...protein for downstream applications Below, we describe a few of the most common tags available. Maltose...via applications such as western blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is ...

  2. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...database describing nearly 145,000 cell lines. Cellosaurus Cellosaurus seeks to identify and describe all ...authors of the paper in which the cell line was described. Using Cellosaurus Cellosaurus is searchable by...Cellosaurus received a report that HT-55, which was described as a colon cancer cell line, is actually a rectal...

  3. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...used for western blot, flow cytometry, and immunofluorescence . However, protein purification is not ideal...for immunoprecipitation, western blot, and immunofluorescence with some success for protein purification...and can serve as an epitope tag as well as a fluorescent marker. The GFP tag is ideal for live cell imaging...imaging, FRET, flow cytometry, or immunofluorescence where an antibody can’t be used or that step can ...

  4. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...mCherry fluorescent protein followed by a stop codon and the coding sequence for a green fluorescent protein...representation of cell-free system, capable of producing fluorescent proteins and enzymes. Image from Moore et al....validated through monitoring the expression of fluorescent proteins and oxytetracycline enzymes. Next, this...

  5. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...recent contribution to the field is a series of fluorescence resonance energy transfer (FRET)-based calcium...with a solution: they devised self-associating fluorescent domains whose association is disrupted in the...Robust red FRET sensors using self-associating fluorescent domains. ACS Chem Biol. 2013 Oct 18;8(10):2133...et al. MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor. PLoS One. 2013 Dec 2;8(12):e82009...

  6. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...see: Uhlen et al., 2016). In this post, we will describe the approaches that can be used to determine selectivity... cells were labelled with a green or violet, fluorescent dye, respectively. WT and KO cells were mixed...through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely with target...recognized by either antibody was not shared. Tables describe that the antibodies both recognized the target...use in western blot, immunoprecipitation, immunofluorescence and flow cytometry. F1000Research, 13, 817...

  7. Hot Plasmids - January 2023

    Type
    Blog Post
    ...speed of light over the last decade, but the fluorescent proteins (FPs) necessary for many of these applications...light and electron microscopy and observed high fluorescence resilience to these preparations. After crystalizing...    Fig. 4: hfYFP retains folding and fluorescence in high molarity GdnHCl, at high temperatures...!   Campbell, B.C., et al. Chemically stable fluorescent proteins for advanced microscopy. Nat Methods...

  8. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...Choose the Brightest Fluorescent Protein Resources on Addgene.org Find Fluorescent Protein Plasmids Check...mRNA this displacement leads to a change in the fluorescent signal: untranslated RNAs appear yellow, and ..., which contains the tethering sites for the fluorescent proteins and is driven by an inducible promoter...

  9. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...Requirement and Expanding CRISPR Beyond SpCas9 described the PAM specificity of Cas9s isolated from different..., and NGCG). Since then, the Osamu Nureki Lab described a rationally engineered Cas9, SpCas9-NG, which...Nishimasu et al., 2018). The David Liu Lab similarly described xCas9, which displayed the ability to target multiple...post on choosing a Cas9, the Feng Zhang Lab had described eSpCas9, while the Keith Joung Lab had developed...

  11. Hot Plasmids - May 2022

    Type
    Blog Post
    ... factors, including limitations of number of flourescent proteins that can be inserted into a cell without...application. Each visual barcode consists of a fluorescent protein coupled to a specific subcellular localization...different localization peptides with four different fluorescent proteins, and inserting each visual barcode into...and reporters that drive the expression of the fluorescent protein by a specific transcription response ...

  12. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...process is called α-complementation. The system described above was put to practical use in the following...α-complementation cloning MCS): pGEM-T, pUC18 and pUC19, and pBluescript are a few common vectors. Blue-white screening...dependent on certain media components, and are rescued by genes supplied on the transformed plasmid. Such...complement auxotrophic cell lines), or genes which rescue a lethal phenotype. Resources on the Addgene Blog...

  13. dTAG - You're it!

    Type
    Blog Post
    ...locus-specific knock-in of FKBP12F36V. Detailed protocols describing the use of these plasmids are available here....al. and Weintraub et al.), chemical and genetic rescue experiments (Nabet et al.), proteomics (Nabet et...degradation, and lead author on the recent paper describing the dTAG technology. Follow him on twitter @behnamnabet... 29581585. This reference contains the full description of the dTAG system. The dTAG system was employed...

  14. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...includes overview information, key dates, funding description, research plan, award information, eligibility...aspects are understood. While reading the funding description, pay attention to the keywords used and think...grant applications restrict the space available to describe the proposed work. Thus, every single sentence...project are clearly stated and align with the description of the RFA and funding institution. Emphasizing...

  15. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...works best with western blots that use chemiluminescent or fluorescent detection, like horseradish peroxidase...that involves adding fresh chemiluminescent substrate or imaging your fluorescent probe. Then, if you don...

  16. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...cloning, the Haynes lab’s Protocols.io protocol describes an elegant system that allows for the assembly...implications for the fusion protein effectivity), and a fluorescent protein tag for visualization (Fig. 1b). While...catalytically inactive dCas9) to modify chromatin. A fluorescent protein can still be included for visualization...quantify protein degradation the Ward lab used fluorescent proteins (FPs) fused to the AID* (minimal 44 ...

  17. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...Green or Taqman for signaling. Sybr Green is a fluorescent dye that works by binding to all double-stranded...while Taqman uses an intercalating dye, with a fluorescent probe and quencher to quantify specific amplicons...materials, and is not particularly sensitive. The fluorescent signal can be affected by AT-rich DNA and the... Sybr Green chemistry. Blue arrows represent fluorescent signaling in the presence of dsDNA. Image courtesy...

  19. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ...made news in March with a publication in Science describing the development of “transcriptors,” transistor-like... and observed that we humans find it useful to describe and interact with the world using words like OR...The future of synthetic biology Addgene: You've described the pieces in these kits as the beginnings of ...regulation of gene expression including growth or fluorescence. There are a few examples of engineered RNA ...

  20. Hot Plasmids - November 2023

    Type
    Blog Post
    ...LOV-Turbo plasmids here! Next, Qin and Cheah et al. described TransitID, where two proximity labeling enzymes...slow-growing protein fiber in the cytoplasm and can be fluorescently labeled. Using lentiviral vectors to stably ...different promoters, specific time points appear as fluorescent bands in the fiber, resembling a “ticker tape... treated with a phorbol ester activator gave fluorescent signal in the ticker tape from cFos::GFP-iPAK4...
Showing: 221 - 240 of 711 results