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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 638 results for: Erf

Showing: 221 - 240 of 638 results

  1. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...can be combined with CRISPR/Cas9 to efficiently perform genetic manipulations in animal models or cells...activity. In summary, the piggyBac™ transposon is a powerful technology that may be used for a variety of research...piggyBac™ for gene editing allows you to efficiently perform seamless genetic manipulations, while avoiding ...

  2. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...Read on for an in-depth breakdown of how to do perform restriction digests.   Overview of the restriction... common chemically-competent cells. You should perform, at minimum, two transformations after a ligation... 101 Posts Resources on Addgene.org Find the Perfect Empty Vector for Your Cloning Experiments Browse...

  3. Pipetting Protocol

    Type
    Protocol
    ...the Volume on the Pipette Although each pipette performs the same function, the numbers are read differently...

  4. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...what I think are the main tips for each step of performing and analyzing a gene edit using Addgene’s lentiviral...lentiCas9-blast. Anti-Cas9 Western blotting was performed on whole-cell extracts from stable pools of A549...blasticidin selection. Anti-Cas9 Western blotting was performed on whole-cell extracts from the initial stable...

  5. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...phenotypes. These approaches may become even more powerful following a report that one can use linear repair...non-linked loci; pha-1(e2123) mutant worms are perfectly viable at 15 ºC, yet exhibit complete embryonic...recombination repair pathway. Reagents required to perform pha-1 co-conversion are available through Addgene...

  6. Finding Your Science Policy Path

    Type
    Blog Post
    ...and grow along the way. That’s what makes it so wonderful. The most challenging part of jumping into this...me, the Veterans Health Administration was the perfect position because my science policy ‘why’ was to...this question may change over time and that’s perfectly normal!  Action Items:  Start a career journal...

  8. Pouring LB Agar Plates

    Type
    Protocol
    ...technique if you are working with any weird and wonderful organisms. While your samples are sterilizing ...

  9. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...homologous recombination has not occurred. This powerful negative selection system ensures high editing...Transcriptional repression (CRISPRi) in bacteria As RNA interference does not function in bacteria, most efforts ...inMycobacterium tuberculosisusing an efficient CRISPR interference system. Nucleic Acids Res 44:e143–e143 . https...

  10. AAV ddPCR Titration

    Type
    Protocol
    ...Follow this protocol to perform digital droplet PCR (ddPCR) for purified AAV titration....

  12. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    ...long the scientist waited after injection before performing the experiment. Every entry has an accompanying... so that you can see for yourself how the AAV performed in that experimental setup. For example, this ...

  15. AAV Production in HEK293 Cells

    Type
    Protocol
    ...serotypes are harvested at 96–120 h. Optional: perform a media exchange at ~18 h post transfection. Changing...

  16. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...on the floor outside the lecture hall. It was wonderful to see the ad-hoc ‘sciencing’ when Dr. Prakash... By connecting art and science, a fascinating interface is created that produces loads of inspiration ...

  18. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...time needed to go from planning an experiment to performing one.  At Addgene, over 120 labs have deposited...we currently have data for are: cut, activate, interfere, visualize, nick, purify, tag, scaffold, CRISPR-display...

  19. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ... and serotype. Please note that Addgene cannot perform functional or in vivo testing. We recommend you...plasmid of choice for more information on its performance.   Titers We expect titers for plasmids packaged...
Showing: 221 - 240 of 638 results