We narrowed to 327 results for: INO

1. Sharing is Caring: But How to Distribute Open Hardware?

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   Blog Post

   ... available tools such as the Raspberry Pi, the Arduino, or 3D printers are key to enabling this work. ...

2. Antibodies 101: Isotypes

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   Blog Post

   ...have different versions or subclasses that have minor differences in their heavy chain constant domains...

3. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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   Blog Post

   ...lab. Chemicals like calcium phosphate and diethylaminoehtyl (DEAE)-dextra neutralize or even impart an...

4. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   Blog Post

   ...phage recombination machinery is induced using L-arabinose. These cells can be made and frozen in large batches...

5. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   Blog Post

   ...ChIP-Seq results. The FLAG epitope is quite small—20 amino acids—so we don’t think it interferes with the function...

6. Plasmids 101: Blue-white Screening

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   Blog Post

   ... enzyme. Providing DNA encoding this section of amino acids (called the α-peptide) to a lacZΔM15-mutant...

7. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   Blog Post

   ... CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

8. 3 Challenges in Plant Synthetic Biology

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   Blog Post

   ...Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in 2004, she accepted ...

9. Plasmids 101: Yeast Vectors

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   Blog Post

   ...L-lysine yes - Grow on plates containing alpha-aminoadipate in the absence of a nitrogen source. S. cerevisiae...

10. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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    Blog Post

    ... plans. 2 of those raising - the University of Illinois and the University of Michigan - initially cancelled...

11. RANbodies: Reporter Nanobody Fusions

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    Blog Post

    ...that’s encoded on a plasmid. A nanobody is a ~150 amino acid, single domain antibody. To create their RANbodies...

12. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post

    ...protein (mScarlet) with either a seven or eight amino acid linker between them. The Ace-mScarlet voltage...

13. Antibodies 101: Affinity Reagents

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    Blog Post

    ...variety of targets, including proteins, peptides, amino acids, drugs, metal ions, and cells. They can be...

14. What's New in CRISPR - November 2021

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    Blog Post

    ...efficiency. The lab also optimized the architecture and amino acid sequence of the prime editor, yielding PEmax...

15. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post

    ...cell 21.3 (2012): 309-322. PubMed PMID: 22439926. Hoshino, Ayuko, et al. "Tumour exosome integrins determine...

16. Transferable Skills Guide: Cross-team Communication

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    Blog Post

    ...first weeks or months when I was learning the terminology of a new field. Annotate: When creating important...

17. Developing Lab Management Software for Biology

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    Blog Post

    ...about how they would like these things tracked. Terminology and language are tricky Names for things will...

18. Addgene's Tips for Plasmid Quality Control

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    Blog Post

    ... sequence from a typical reaction, or about 250 amino acids worth, so make sure the feature you want to...

19. Plasmids 101: Golden Gate Cloning

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    Blog Post

    ... with an attB recombination scar encoding eight amino acids, but Golden Gate assembly can be designed ...

20. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post

    ...end positions without changing the order of the amino acids in the protein) may be able to boost FRET ...