We narrowed to 319 results for: INO

1. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   Blog Post

   ... CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

2. 3 Challenges in Plant Synthetic Biology

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   Blog Post

   ...Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in 2004, she accepted ...

3. Plasmids 101: Yeast Vectors

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   ...L-lysine yes - Grow on plates containing alpha-aminoadipate in the absence of a nitrogen source. S. cerevisiae...

4. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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   ...cell 21.3 (2012): 309-322. PubMed PMID: 22439926. Hoshino, Ayuko, et al. "Tumour exosome integrins determine...

5. RANbodies: Reporter Nanobody Fusions

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   ...that’s encoded on a plasmid. A nanobody is a ~150 amino acid, single domain antibody. To create their RANbodies...

6. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ...protein (mScarlet) with either a seven or eight amino acid linker between them. The Ace-mScarlet voltage...

7. Antibodies 101: Affinity Reagents

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   ...variety of targets, including proteins, peptides, amino acids, drugs, metal ions, and cells. They can be...

8. What's New in CRISPR - November 2021

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   ...efficiency. The lab also optimized the architecture and amino acid sequence of the prime editor, yielding PEmax...

9. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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   ... plans. 2 of those raising - the University of Illinois and the University of Michigan - initially cancelled...

10. Transferable Skills Guide: Cross-team Communication

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    Blog Post

    ...first weeks or months when I was learning the terminology of a new field. Annotate: When creating important...

11. Developing Lab Management Software for Biology

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    ...about how they would like these things tracked. Terminology and language are tricky Names for things will...

12. Addgene's Tips for Plasmid Quality Control

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    ... sequence from a typical reaction, or about 250 amino acids worth, so make sure the feature you want to...

13. Plasmids 101: Golden Gate Cloning

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    ... with an attB recombination scar encoding eight amino acids, but Golden Gate assembly can be designed ...

14. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    ... editing efficiencies of the chimeric and fully albino mice ranging from 11% to 100%, a level similar ...

15. Mouse Modeling, Part 1: Genetically Engineered Mice

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    ...first blog post in this series, I’ll highlight terminology you should be familiar with before working with...

16. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post

    ...Thomas, D., & Surdin-Kerjan, Y. Metabolism of sulfur amino acids in Saccharomyces cerevisiae. Microbiol Mol...

17. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    Blog Post

    ...doi.org/10.1038/nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

18. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    ...Szikra, T., Esposti, F., Cowan, C.S., Bharioke, A., Patino-Alvarez, C.P., Keleş, Ö., Kusnyerik, A., Azoulay...

19. Hot Plasmids and Viral Preps - March 2021

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    Blog Post

    ...fluorescent proteins (FPs) fused to the AID* (minimal 44 amino acids auxin inducible degron sequence) tagged proteins...

20. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post

    ...end positions without changing the order of the amino acids in the protein) may be able to boost FRET ...