We narrowed to 326 results for: INO

1. Plasmids 101: Yeast Vectors

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   Blog Post

   ...L-lysine yes - Grow on plates containing alpha-aminoadipate in the absence of a nitrogen source. S. cerevisiae...

2. Transferable Skills Guide: Cross-team Communication

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   Blog Post

   ...first weeks or months when I was learning the terminology of a new field. Annotate: When creating important...

3. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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   Blog Post

   ...cell 21.3 (2012): 309-322. PubMed PMID: 22439926. Hoshino, Ayuko, et al. "Tumour exosome integrins determine...

4. RANbodies: Reporter Nanobody Fusions

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   Blog Post

   ...that’s encoded on a plasmid. A nanobody is a ~150 amino acid, single domain antibody. To create their RANbodies...

5. Antibodies 101: Affinity Reagents

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   Blog Post

   ...variety of targets, including proteins, peptides, amino acids, drugs, metal ions, and cells. They can be...

6. What's New in CRISPR - November 2021

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   Blog Post

   ...efficiency. The lab also optimized the architecture and amino acid sequence of the prime editor, yielding PEmax...

7. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   Blog Post

   ...protein (mScarlet) with either a seven or eight amino acid linker between them. The Ace-mScarlet voltage...

8. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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   Blog Post

   ... plans. 2 of those raising - the University of Illinois and the University of Michigan - initially cancelled...

9. Developing Lab Management Software for Biology

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   Blog Post

   ...about how they would like these things tracked. Terminology and language are tricky Names for things will...

10. Addgene's Tips for Plasmid Quality Control

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    Blog Post

    ... sequence from a typical reaction, or about 250 amino acids worth, so make sure the feature you want to...

11. Plasmids 101: Golden Gate Cloning

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    Blog Post

    ... with an attB recombination scar encoding eight amino acids, but Golden Gate assembly can be designed ...

12. CRISPR Plasmids - Yeast

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    Collection

    ... gRNA. Adenine base editors convert adenine to inosine, which is replaced by guanosine to create A->G ...

13. Protocol - pLKO.1 – TRC Cloning Vector

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    Protocol

    ...Sigma #A9539 D.2 Screening for Inserts Day 1: 1. Inoculate 5 colonies from each ligation into LB + 100 μg...

14. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    Blog Post

    ... editing efficiencies of the chimeric and fully albino mice ranging from 11% to 100%, a level similar ...

15. Mouse Modeling, Part 1: Genetically Engineered Mice

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    Blog Post

    ...first blog post in this series, I’ll highlight terminology you should be familiar with before working with...

16. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post

    ...Thomas, D., & Surdin-Kerjan, Y. Metabolism of sulfur amino acids in Saccharomyces cerevisiae. Microbiol Mol...

17. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    Blog Post

    ...doi.org/10.1038/nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

18. Progress Towards a PAM-Free CRISPR

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    Blog Post

    ...knock-in a patient mutation or need to make a precise amino acid change, there is very little flexibility in...

19. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post

    ...end positions without changing the order of the amino acids in the protein) may be able to boost FRET ...

20. Hot Plasmids February 2024

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    Blog Post

    ...the epitope tags we know and love, the FLAG tag (amino acid sequence DYKDDDDK) may be one of the most versatile...