Skip to main content
Addgene
Showing: 221 - 240 of 471 results

  1. Summer SciComm: Preprints

    Type
    Blog Post
    ... Biology in a position working with preprints in life sciences. He also posted two preprints of his own...proposal for the future of scientific publishing in the life sciences. PLoS Biol. https://doi.org/10.1371/journal.pbio...

  2. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...secondary antibody is ideal as it will help to amplify the signal. Some applications use either direct...that a secondary should be used when signal amplification is needed.  Species  Next, check the species...

  3. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...the scientific method to everyday problems in your life. This tactic is a good way to see how transferable...resolve issues effectively does not mean I am qualified to be an astronaut. But, knowing that this skillset...

  4. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...antibodies are polyclonal, as their function is to amplify the signal from a primary antibody; the ability...target protein thereby greatly increasing signal amplification.  When to stay away from polyclonal antibodies...

  5. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...information you need to choose well. What are their qualifications? Do they have certifications? How can they ...posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet chats, has an outstanding ...

  7. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...virus-based experiment at one point or another. After amplifying a viral vector, collecting virus, and transducing...replication. To avoid genomic rearrangements, try amplifying the viral vector with bacteria designed to minimize...

  8. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...processing and contributes to variability in RNA half-life, and ultimately gene-expression. Polyadenylation...with reduced viral titer, but improved transcript life, and so should be handled with care (4). For this...

  9. A Look at Addgene's QC Process

    Type
    Blog Post
    ... utilize an array of tools that rely on DNA amplification and manipulation to produce and edit plasmids...places where DNA is being created, such as PCR amplification or bacterial growth. Created with BioRender....

  11. Lab Automation at Addgene

    Type
    Blog Post
    ...wishing there was a machine that could make your life a hundred times easier. We’ve certainly felt this...Additional Resources on the Addgene Blog Apps for Lab Life Developing Lab Management Software for Biology Tips...

  16. Of Myc and Men

    Type
    Blog Post
    ... those questions in this post as we explore… the life and times of the myc tag.  Origins of protein tags...would help the field explore Myc’s role in cell proliferation and tumorigenesis.  Using two different synthetic...

  17. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...Resources I asked some colleagues to share valuable life lessons they have learned during their scientific...wrote about them in a blog post on Born to Science: Life Lessons from Scientific Training More and more ...

  18. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...required by MMEJ can easily be added via PCR amplification. Given the inefficiency of HDR for knock-ins...Select for puromycin resistant cells Step 5: PCR amplify and sequence the locus to verify correct GFP-puro...

  20. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ... P0), infecting new Sf9 cells, and getting an amplified P2 virus. This P2 virus can be produced in a greater...Baculovirus replicates inside insect cells, and its lifecycle ends in cell lysis, releasing the virus to the...
Showing: 221 - 240 of 471 results