We narrowed to 525 results for: MEA
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TypeBlog Post... a result, mesothelioma is often misdiagnosed, meaning that by the time it has caught it has usually started...in the body. Such misdiagnosis and late discovery mean that most mesothelioma patients have an extremely...
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Plasmids 101: Shuttle Vectors
TypeBlog Post...transient transfection, the vector remains episomal, meaning it stays separate from the genome. One option for...replicate during the course of your experiment, meaning you don’t have to worry about vector replication... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog Post...frameshift or 21+ bp indel. This score is a useful measure to determine how many of the contributing indels...frameshift or 21+ bp indel. This score is a useful measure for those who are interested in understanding how... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...plant sciences “transient transformation” usual means that neither a stable cell line or plant is regenerated...GUS and are therefore good temporal reporters to measure promoter activity, for example. Useful positive... -
Lab to Office Culture Shock
TypeBlog Post...think “scientist.” But leaving the bench does not mean you’re not a scientist anymore. Being a scientist...their entire career. Yet even success in academia means you’ll eventually end up as a PI working what is... -
Supporting Reproducibility with a Connected ELN
TypeBlog Post...it’s difficult to get data out of the ELN. This means that reproducibility is limited in two ways – only...external file storage resources remain live. This means that someone who wants to query and attempt to reproduce... -
How to Be an Excellent Trainee
TypeBlog Post...you remember everything? Write it all down! And I mean everything! The bare minimum should be taking notes...invaluable. With a little thought you can build meaningful relationships in your new lab, learn the required... -
Plasmids 101: Codon usage bias
TypeBlog Post... with one of these tools, it doesn’t necessarily mean the gene is going to express well. If you do get...PMC3565066. 7. Quax, Tessa EF, et al. "Codon bias as a means to fine-tune gene expression." Molecular cell 59.2... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...Share science, video usurps the written word as the means for communicating research. Short videos of 3 to... helps researchers go beyond citation rates to measure the impact of their contribution. It offers a 5... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...challenging. The fast paced nature of projects often means working long hours both at home and at the office...and educational parts of the job. Is consulting meant for PhDs? Healthcare consultants are usually trained... -
The Challenges of Cell Culture
TypeBlog Post...experiments carried out on low passage cells and this means you can regularly discard the cells you are working... actual flasks of cells in the incubator). This means any other scientist who wishes to use these cells... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...should be available for anonymous request. This means that supporting datasets must be logically annotated...will be in danger of having to deliver on well-meaning but impractical requirements imposed by publishers... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post...recognize and bind to specific promoter elements. This means that the promoter present in your plasmid backbone...bacterial and mammalian promoters. These lists is by no means exhaustive, but should be a good place to start ... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post...secondary antibodies are generally polyclonal, which means that multiple secondary molecules can bind to a ...target and sample type. Example steps include: permeabilization, antigen retrieval, and sectioning. Blocking... -
Transferable Skills Guide: Time Management
TypeBlog Post... be aware of deadlines and think about what they mean in terms of the utility of your tasks. In my cell...accomplish these goals. Practically speaking, this means I write everything in a Word or google doc that ... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...are that it does not use ubiquitous promoters, meaning that not all cell types can be labeled, and that...labels Drosophila neurons at the neuroblast stage, meaning that neurons of the same lineage will express the... -
Tips for Getting a Faculty Position
TypeBlog Post...rapidly dispelled. She would ask me, "Is this what you mean? Are you trying to say this?" I'd say, "No, I'm ...a lab, getting grants, and writing papers? This means that once you get an interview, your previous success... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...proteins can be accurately determined in vitro by measuring the fluorescence intensity of purified protein..., Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in single cells." PloS one 13.3... -
Evolution of Lab Techniques
TypeBlog Post...DNA strand as it slips through a tiny pore by measuring differences in ion flow caused by the different...nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing could move from sequencing ... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...β-galactosidase activity abolished lacY Lactose permease inactivated, lactose cannot be taken up by cell...common lab strains; however, these tables are by no means exhaustive! For a more comprehensive list and additional...