We narrowed to 423 results for: NUC

1. Genome-wide Screening Using CRISPR

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   Blog Post

   ...non-specific endonuclease (Cas9) and a single stranded guide RNA (gRNA). The ~20 nucleotide targeting sequence...

2. An Introduction to Adenovirus

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   ...innate immune system; and reconfiguring of the nuclear compartment, gene expression, and protein translation...Adenovirus Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https...

3. Viral Vectors 101: The Retroviral Lifecycle

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   ..., W. S., Pitkanen, A., & Rubin, H. (1965). The nucleic acid of the Bryan strain of Rous sarcoma virus:...purification of the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences...

4. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   ...VchINT does not recruit other nucleases (such as Cas3) or have its own nuclease activity. Instead, it forms...

5. CRISPR Antimicrobials

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   ...antimicrobials using efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed... Bikard, David, et al. “Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials.” ...

6. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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   ...translocation trap (ITT) system to analyze signal-induced nuclear translocation of proteins in 2004. In addition,...addition, we have recently developed the oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method to suppress...

7. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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   ...profiling of off-target cleavage by CRISPR-Cas nucleases." Nature biotechnology 33.2 (2015): 187. Pubmed.... 3. Fu, Yanfang, et al. "Improving CRISPR-Cas nuclease specificity using truncated guide RNAs." Nature...

8. Deciphering the Mysteries of Behavior with Viral Vectors

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   ...Previous research has shown that regions such as the nucleus accumbens (NAc), which is linked to the reward ...cytokine interleukin IL-1 beta in the dorsal raphe nucleus (DRN), a region thought to be involved in these...

9. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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   ...been demonstrated in zebrafish using zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another...deactivation method in zebrafish are short, modified oligonucleotides called morpholinos. When injected into zygotes...

10. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    Blog Post

    ...take the nucleus and put it in a fertilized egg to get edited embryos (replacing the nucleus is still ...

11. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    Blog Post

    ...that MECP2 binds to methylated CpG dinucleotides and CAC trinucleotides during neuronal maturation (Tillotson...

12. CRISPR-mediated Plant Base Editors

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    ... base editors probably due to the presence of nucleosome or other proteins. You can detect base editing...PMC4873371.  2. Nishida, Keiji, et al. "Targeted nucleotide editing using hybrid prokaryotic and vertebrate...

13. Harnessing Bacterial Toxins for Allelic Exchange

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    ...unfolding and sliding at upstream standby sites." Nucleic acids research42.4 (2013): 2646-2659. PubMed PMID...selection systems for unmodified Enterobacteriaceae." Nucleic acids research 43.13 (2015): e83-e83. PubMed PMID...

14. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    ...fluorescence. In Brainbow 2.1, GFP is tagged with a nuclear localization sequence, allowing it to localize ...mouse generates cells that randomly express either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic...

15. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    ...results in a polymerase error rate of up to 2% per nucleotide position. The “sloppy” PCR reaction is created...degraded by uracil DNA glycosylase (UDG) and Exonuclease III (ExoIII) (step 4). UDG recognizes uracils...

16. Plasmids 101: Common Lab E. coli Strains

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    ... of certain expressed proteins endA, endA1 Endonuclease I (nonspecific cleavage of dsDNA ) mutation ...recombination, increases plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability...

17. Antibodies 101: Multiplex Immunofluorescence

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    ...include a marker like the Hoechst stain for the nuclei. (See Brewer et al, 2022 for a sample protocol.... (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail...

18. Plan Your Experiment

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    ...increase or decrease depending upon the specific nucleotides within the selected target sequence. Additionally...the Cas enzyme and gRNA, or as a functional ribonucleoprotein (RNP). Created with BioRender.com. When choosing...delivery Lastly, you can deliver Cas-gRNA ribonucleoprotein (RNP) complexes directly into your target ...

19. Important Considerations When Using AAVs

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    Blog Post

    ...hairpin RNAs (shRNAs), antisense oligonucleotides (ASOs), zinc finger nucleases, and many others (12). The...

20. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    ...eukaryotic process where approximately 20-30 nucleotides of double-stranded RNA (dsRNA) results in downregulation...functional APT will convert 2-FA to cytotoxic nucleotides, resulting in death. In the presence of 2-FA,...