We narrowed to 417 results for: NUC

... dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes without editing the...expression using an engineered CRISPR-Cas system. Nucleic Acids Research 41:7429–7437 . https://doi.org/10.1093...

...mutations, frame shifts, missing elements, and nucleotide indels or mismatches in significant plasmid elements... reference; and identifying mixed plasmid and nucleotide populations. All of these topics require additional...

...protected by histone-like proteins forming the nucleocapsid. B) Three generations of adenoviral vectors:...purified plasmid stock should be analyzed by endonuclease restriction digest or fully sequenced to confirm...

...synthetically made RNA (riboprobes) with modified nucleotides (for example, Digoxigenin-labelled Uracil) that... subsequently destroyed by Argonaute-catalyzed nuclease activity or their translation disrupted through...

...primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely due to its long,...Cell-penetrating Cas9, fused to HIV TAT, Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess...

...overhangs with the same three nucleotides in a row; (4) no more than two nucleotides in the same position; (...

... "WDDD: worm developmental dynamics database." Nucleic acids research 41.D1 (2012): D732-D737. PubMed ... a platform for 3′ UTR biology in C. elegans." Nucleic acids research 36.suppl_1 (2007): D57-D62. PubMed...

...administration. However, until recently safe delivery of nucleic acid cargo (DNA or RNA) to target cells has been...Self-complementary AAV mediates gene targeting and enhances endonuclease delivery for double-strand break repair." Gene...

...constructs for visualizing cellular components (nucleus, endosomes, lysosome, golgi, ER, inhibitory synapses...TnpB (A) and Cas9 (B) genome editors. Effector nucleases are shown bound to the target DNA, recruited by...

...ends. These fragments are then combined with an exonuclease, a polymerase, and a DNA ligase to carry out ...When mixed, topoisomerase I provides both the endonuclease and ligase function while 5’ adenosine overhangs...

...cells, the molecular circuitry of pluripotency and nuclear reprogramming. Cell, 2008. 132(4): p. 567-82. PubMed... S. and D. Wolf, Totipotency, pluripotency and nuclear reprogramming. Adv Biochem Eng Biotechnol, 2009...

...including mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs ...power of this system lies in the ability of the endonuclease Cas9 to create a DNA double stranded break (...

...endA+. endA encodes a thermostable periplasmic endonuclease that can sometimes co-purify with your plasmid...recommend performing an extra wash step to remove endonucleases (see figure at right for an example). Look out...

...non-specific endonuclease (Cas9) and a single stranded guide RNA (gRNA). The ~20 nucleotide targeting sequence...

...VchINT does not recruit other nucleases (such as Cas3) or have its own nuclease activity. Instead, it forms...

...innate immune system; and reconfiguring of the nuclear compartment, gene expression, and protein translation...Adenovirus Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https...

..., W. S., Pitkanen, A., & Rubin, H. (1965). The nucleic acid of the Bryan strain of Rous sarcoma virus:...purification of the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences...

...antimicrobials using efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed... Bikard, David, et al. “Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials.” ...

...translocation trap (ITT) system to analyze signal-induced nuclear translocation of proteins in 2004. In addition,...addition, we have recently developed the oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method to suppress...

...that MECP2 binds to methylated CpG dinucleotides and CAC trinucleotides during neuronal maturation (Tillotson...