We narrowed to 443 results for: RELA

1. Antibodies 101: Reading a Flow Plot

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   Blog Post

   ...population. Heat maps are often used to indicate the relative density of cells. You can see both of these features...intracellular IFNγ, IL-17 and IL-10 producing T cells correlates with the occurrence of post-transplant opportunistic...

2. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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   ...patients are reprogrammed less efficiently, no such correlation has been found between the age of the patients... amplification in the host cell. This enables relatively high and long-term expression of the reprogramming...

3. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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   ...construct [1,2], i.e. positioning of the FP sequence relative to cell compartment targeting sequences, which...to characterize FP-fusion proteins functionally relative to the untagged protein of interest. To avoid ...

4. Antibodies 101: Introduction to Immunofluorescence

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   ...multiplexing is that you can ask how multiple proteins relate to one another in the same sample. For this strategy... The biggest difference between IF and ICC/IHC relates to the type of secondary antibody you use, so as...

5. Antibodies 101: Single Chain Fragment Variables (scFvs)

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   ...scFvs are also used for eye diseases, such as age-related macular degeneration (AMD) since direction injection...cell. When a CAR has an scFv that binds cancer-related antigens, binding of the scFv part of the receptor...

6. 10 Steps to a Perfect Science Talk

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   ...work does relate, make sure to include that material, but don’t force it. You can always relate to scientists...

7. Thoughts on the Future of Research 2015 Boston Symposium

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   ...provided as an explanation for why they are paid relatively low wages (Stephan estimates that postdocs are...that PIs no longer save grant money by paying relatively low postdoc salaries as opposed to Staff Scientists...

8. CRISPR 101: Epigenetics and Editing the Epigenome

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   ...general, epigenetic chromatin modifications are correlated with alterations in gene expression, but causality...tools for this purpose. Epigenetics began as a correlative field in which covalent modifications to DNA ...

9. Viral Vectors 101: AAV Variables That Matter

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   ...consider. First, the titer required is inversely related to transduction efficacy: the higher the efficiency...against the Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated Transgene...

10. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    ...2023; Wu, Asokan, et al., 2006). AAV9 AAV9 has relatively broad tissue tropism and high transduction efficiency...retinal cells (Issa et al., 2023). AAV11 AAV11 is a relatively recent addition to the toolbox, with the first...

11. Finding Your Perfect Job After University

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    ...left me optimistic that I would find a genetics related role soon after finishing my MSc. The masters program...I had received all the training and experience relating to good manufacturing practice (GMP) and good ...

12. Important Considerations When Using AAVs

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    .... Speed: AAV transduction experiments are relatively quick. We have found that expression occurs quickly...AAV selection, there are three important factors related to location. Location (1): What tissue? Location...

13. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    ...high Cas9 MOI considering that A549 cells are relatively easy to transduce. I ended up with too many Cas9...on genomic DNA. The surveyor assay itself was relatively easy. Figure 4: Detection of edited BRAF...

14. Deep Dive: Statistical Tests (Comparisons)

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    ... controls how many false positives are allowed relative to true positives. Both metrics can be used to...and plot your data in ascending numerical value relative to quantiles derived from a theoretical distribution...

15. A Guide to Getting Started in Undergrad Research

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    ...neurodegenerative disease because they had close relatives suffering from similar diseases. Others were inspired...t know yet has helped me cultivate stronger relationships with my labmates. Sometimes what I thought was...

16. Custom CRISPR Screens & the Green Listed Software

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    ...your own favorite Reference Library, for example related to strategies beyond SpCas9, or species not currently... other species, and libraries using strategies related to other nucleases as well as inhibitory and activating...

17. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

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    ...efficiency of Cas9 cleavage is relatively high while the efficiency of HDR is relatively low, meaning that most...

18. Delivery Methods for Generating iPSCs

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    ... generating iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail.... The advantages of this method are that it’s relatively simple to implement and doesn’t require time-...

19. CRISPR Guide

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    Collection

    ...insert donor DNA at “landing sites” defined by relatively short sequences. Some have been paired with CRISPR...

20. New FLIS Plasmid (April Fools 2022)

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    Blog Post

    ...rather astonishing ability to...well, annoy. Fly-related Issues in the Lab A significant drawback of working...