We narrowed to 583 results for: Ret;

1. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

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   ...speechless (the club of people who have been in space is pretty small). Therefore, I was delighted to be invited...During the panel, an artist was sketching her interpretation of the discussion in markers on a whiteboard...

2. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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   ...protein-protein interactions through multicolor labeling and FRET. Specialty constructs for biosensing. Over 300... tomato) and the photoactivatable fluors (e.g. Phamret and Dendra). It’s just such a great collection ...

3. Bringing Sustainable Practices to the Lab: Recycling

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   ...Their cooler boxes come with a prepaid return shipping label. To return the box, simply peel your address ...

4. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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   ...     Additional Resources Learn how you can use FRET to study protein interactions Learn about other ...CRISPR plasmids for isolating a genomic locus Browse FRET plasmids for studying protein interactions  ...

5. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

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   ...graduate students, post-docs, and PIs. I won’t pretend that that there aren’t problems with the academic...academic enterprise or that there weren’t some pretty hefty bumps during my graduate training. I particularly...

6. Single-cell tracking of lineage and identity with CellTag

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   ...cell types. MEFs were reprogrammed into iEPs by retroviral expression of transcription factors Foxa1 and.... Adding Mettl7a1 to the standard Foxa1-Hnf4α retroviral overexpression cocktail yielded a three-fold ...

7. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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   ...PubMed PMID:29109157. Nanobody toolkit to monitor retrograde transport at the cell surface Article contributed...developed a set of functionalized nanobodies to study retrograde transport from the cell surface. Their toolkit...

8. The Strength of Story Telling

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   ...be the first author. When I raised this, he was pretty angry and started arguing with me. First I calmed...jumping backwards in time to explain something, rethink your structure. Develop your protagonist In most...

9. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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   ...not require any additional reagents or lab work (Pacouret, 2017). Moreover, the sequencing data from multiple...195–205 . https://doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha M, Shelke R, Andres-Mateos E, Xiao...

10. Antibodies 101: Chimeric Antibodies

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    ...In most cases, the resulting chimeric antibody retains the binding specificity to the original target ...In addition, chimeric antibodies will inevitably retain some sequence from their parental species which...

11. Donations from Addgene to Yield Answers for Rare Disease Researchers

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    ... XP and melanoma more broadly. “This [award] is pretty great for us,” Wang said. “We were planning on ...mutations in the gene may prevent neurons from returning to basal conditions normally after periods of ...

12. Fluorescent Protein Guide: Activity Regulation

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    ...More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Biosensors Optogenetics...

13. The Importance of High Titer for AAV Transductions

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    ...therefore higher titer will be required. For example, retrograde AAV gets transported up the cell’s axon. While...even larger area, thus necessitating even higher retrograde AAV titers for successful transduction. In another...

14. The MTA at Addgene

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    .... Over the last twenty years, we’ve developed a pretty smooth system for this process. We often get positive... sent the MTA to sign. Once they've signed and returned the MTA, Addgene can move forward with shipping...

15. Plasmids 101: NGS Quality Control for Pooled Libraries

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    ...sequencing is successful, your NGS provider should return results in the form of many, many short DNA sequences...specific libraries, but any analysis script should return the same output: a spreadsheet of read counts. ...

16. Fluorescent Proteins 101: Luciferase

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    ...Peak emission (nm) Size (kDa) Substrate Cofactors Secreted Luminous shrimp(Oplophorus gracilirostris) 455...cell membrane and deliver the substrate; however, secreted luciferase or the use of alternative substrate...

17. Michael Davidson Fluorescent Protein Collection

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    ...More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics The lab of...

18. Depositor Collections

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    ...Neurodegenerative Disease Initiative (iNDI) Collection Rett Syndrome Plasmid Collection and Resource Center ...

19. 15 Years of Addgene: The Top 15 Plasmids

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    ...envelope protein for producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids...as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates...

20. Improved Plasmid Maps Powered by SnapGene

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    ... and sequence displays are now much easier to interpret and analyze at a glance. For a quick look at just...“Analyze Sequence” button. The maps hosted here retain all the features shown in the static maps discussed...