We narrowed to 612 results for: Ret;

... mechanism to culture only bacteria which have retained a functional copy of your plasmid of interest,... plates due to the fact that beta-lactamase is excreted. Satellite colonies are small colonies of bacteria...

...appear reduced in ExM, which could impact how you interpret a target’s structure. For example, cytoskeletal..., Chen F, Piatkevich KD, et al (2016) Protein-retention expansion microscopy of cells and tissues labeled...

... bathroom mirror pep talk, forgotten and then retrieved your lucky socks, persevered through a nerve wracking... snapshot of the skills that you can bring into pretty much any other workplace. To get your mind working...

... (Kyndt et al., 2015)! It sounds simple, and it pretty much is, but there are some tips and tricks you...having an analyzable transgenic in hand, so it is pretty important to get this process right the first time...

...Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own proteins, making the purification...production of Fab fragments where variable chains are secreted into the periplasmic space to be oxidized and ...

...proteins were successfully expressed as fusions that retain their individual activities (1). Researchers also...DBD) and transcriptional activation (TA) domains retain their individual activities when separated (2),...

...grow. As the bacteria divide, they replicate and retain the plasmid. This allows us to make unlimited copies...deposit. Using barcoded tubes is part of Addgene’s secret to success. It helps us quickly process samples...

...kill your productivity. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/11...innovation on your team. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/07...

...exported to the periplasm of bacteria where it can be secreted into the supernatant. Four plasmids containing...in human pluripotent stem cells(hPSCs), Lindy Barrett’s lab at the Broad Institute developed fluorescently...

...trainee (even if you’re an oldbe trainee!) can be pretty nerve-wracking. So how can you be the best trainee...gives twofold benefits: you may learn faster and retain more information plus your mentor will get you ...

...cell’s genome, so no special sequence is needed to retain the vector as cells divide. In transient transfection... and eukaryotes. Conclusion I still think it’s pretty incredible that my cells “speak” the same molecular...

...typically contains one viral genome. Other than a theoretical maximum value, physical titers don’t necessarily...different sources in the same experiment, consider retitering both AAV in your lab. The absolute titer values...

...known 'DNA adenosine deaminases' which would theoretically give rise to an adenine base editor. A massive...developed using the hAPOBEC3 deaminase can edit in a stretched window ranging from 1-17 bp relative to the PAM...

...antibodies. In contrast, with their many epitopes, smFPs retain their ability to be detected after sample preparation...the Addgene Blog Rabies and neuronal tracing Retrograde AAV: Making the journey from axon to nucleus ...

...faculty, students, private sector researchers, or pretty much anyone applying the scientific method to a..., also known as the science videographer, is a retired botanist and oceanographer who has a blog and several...

...pCAG-CreERT2 #14797). These Cre/ FLP versions are retained in the cytoplasm until estrogen receptor ligand...FLEX-vectors Learn More about Cre-Lox Check out Retrograde AAVs for Neurscience Research Resources on Addgene.org...

..., A., Garnier, A. “Downstream processing of oncoretroviral and lentiviral gene therapy vectors.” Biotechnology...conditions on the production of lentiviral and retroviral vectors.” Human Gene Therapy Clinical Development...

...salts may be preferable if the protein product is secreted to the medium or if slow expression is required...required due to solubility concerns. Insoluble and Secreted Proteins: The most common purification protocols...

...pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE...

...composition.  So back to the drawing board.  Cycle 3: A Return to HQ Figure 3: Even Blugene is practicing...acquire gear that’s only good once. Even when things return to relative normalcy, I’ll be incorporating that...