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Showing: 221 - 240 of 601 results
  1. Microbiology Resources

    Type
    Collection
    ..., or by suggesting plasmids for the collection. Return to top Plasmid Kits for Microbiology Kits are sets...therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression - Pertz Lab...common S. cerevisiae lab strains - Ralser Lab Return to top Resources for Microbiology General CRISPR...Genome Database (SGD) (Link opens in a new window) Return to top Do you have suggestions for other plasmids...
  2. Plant Plasmids and Resources

    Type
    Collection
    ... genome editing logistics - Jose-Antonio Daros. Return to top Empty Backbones The table below highlights... Plasmid Description Promoter Cloning Method PI Return to top Fluorescent Proteins The table below highlights...Guide for more. ID Plasmid Description Promoter PI Return to top Other Resources Addgene Blog Posts Addgene...Arabidopsis thaliana based on RNA-seq analysis. Return to top Do you have suggestions for other plasmids...
  3. Biosensor AAV Preps

    Type
    Collection
    ...filtering options. *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from the Karpova... dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? ...viruses would be helpful to the scientific community. Return to top...
  4. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...few commonly available different fragments that retain antigen binding (Figure 2):  F(ab')2 - These fragments...fragments are produced by proteolytic digestion that retains an antibody’s disulfide bonds within the hinge ...
  5. RNA Extraction Without A Kit

    Type
    Blog Post
    ... and RNA is retained in the top, aqueous layer, while DNA and other proteins are retained in the interphase...
  6. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...integrate your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic delivery and have...to permanently induce expression? Check out our Retro expression vector and Lenti expression vector. ...
  7. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...growth factor-β signaling, possibly inducing the secretion of growth promoting proteins, such as human growth...microenvironments! Take myoepithelial cells for instance. They retard tumor formation by inhibiting cell growth, invasion...
  8. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...for cellular delivery of RNAs By Mike Lacy The retroviral-like protein PEG10 specifically binds its own...demonstrate SEND as a modular system for packaging, secretion, and delivery of specific RNAs, such as Cre mRNA...
  9. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...fiction story that doesn’t require any detailed retention of information or formulation of new knowledge...mental bandwidth.  Jargon Learning, using, and retaining new vocabulary takes time and effort (otherwise...
  10. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...plan for a career outside academia (that would be pretty much all scientists), a LinkedIn profile is absolutely...rules of good resume writing. Include examples of concrete accomplishments in both the technical and “plus...
  11. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...gender analysis and 2) provides case studies with concrete examples of how gender analysis leads to discovery...design. But eyes have been opened—and we cannot return to a world that ignores gender. Many thanks to...
  12. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ... body and thereby correct the mutations using retroviral vectors. However, many diseases require techniques...untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the human gene...
  13. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain...
  14. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...particularly effective as it can result in the retention of introns or the removal of exons. Disrupting...microRNA lead to its depletion and Drosha processing retardance. RNA Biol. 2014;11(10):1243-1249. doi:10.1080...
  15. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...resistance genes to help cells survive their own secretions. Pro tip! Historically, colicin-producing plasmids...Resources References Demanèche, S., Bertolla, F., Buret, F., Nalin, R., Sailland, A., Auriol, P., Vogel,...
  16. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...access it later. Pro tip Checking the number of retweets, likes, and comments on a tweet about a paper ...papers are connected via citations, in order to return results that are more relevant to your search terms...
Showing: 221 - 240 of 601 results