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We narrowed to 353 results for: SAM activators

Showing: 221 - 240 of 353 results

  3. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...scientific publishing process. There is a rich and active community of science communication professionals...aspects of bioscience into context, while at the same time working through the details of experimental...

  4. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...untagged control could enter cultured cells and activate expression of a luciferase reporter. CPP was not... better than immortalized lines derived from the same cell type; however, their usage has been limited...

  6. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...product, there can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies are produced...produce the same signal, so only one HRP-conjugated RANbody can be used at a time. Samples can be sequentially...fluorescently labeled tyramide molecule into a highly reactive radical, which is then covalently linked to residues... lower background and better penetration of the sample due to the smaller size of RANbodies compared to...sequentially stained with two HRP RANbodies if the sample is stripped. HRP-RANbodies are, however, compatible...it is approximately as sensitive as detecting the same antigen with a standard primary/labeled secondary...staining of tissue because they have limited cross-reactivity with commonly used anti-rodent or -rabbit secondary...

  7. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...HRP, a chemiluminescent conjugate that can be activated to emit light with a simple kit, and is readily... the antibodies time-sensitive, using precious samples, or being compared to other data in a manner that..., simple to use, not sensitive to light in its inactive state, and cheap. Unless there’s a specific reason...signaling molecule, that all need to be detected in the same assay. This is one of the most complex subjects ...

  8. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ...Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Nat Protoc 12:828–863 . https://doi.org...holes are then filled with other samples in our queue such as viral samples for Viral Genome Sequencing (...preps and pooled libraries.  Viral samples are filled into the same process as our normal Complete Plasmid...unique barcodes per sample so we can assign sequencing reads to the correct sample. The PCR is then performed... process that yields high-quality isolated DNA samples of sufficient quantity for sequencing. This process...yielding anywhere from two to six plates of 96 samples per week. This is where our new process will begin...of a pipeline that takes the raw data from each sample in our pool and individually assembles the reads...

  9. To Each HIS Own

    Type
    Blog Post
    ...variety of ways. How do scientists focus on the activity or function of a single protein? Oftentimes, they...metal ions are immobilized on a bead or resin and a sample containing the protein of interest flowed through...interest and the metal ions.      Fig. 1: A sample containing a polyhistidine tagged protein of interest...tags are also recommended if you need a very pure sample. Because binding strength is proportional to the...

  10. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...time, we have also demonstrated our technology on active virus, as well as shown that we can detect the ...high and above what would be expected in patient samples (e.g.: blood, urine). Adapting toehold switches...pathogen’s genome as unique barcodes to identify samples containing the virus. Of the 48 sensors, targeting... diagnostic results.  Our approach now detects samples at clinically relevant concentrations (1-3 fM; ... look at viral load in patient blood and urine samples; we showed that we can detect the virus directly...Interview with Previous Collin's Lab Member and Sample 6 Cofounder Michael Koeris Learn How Minigenomes...

  11. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...variety of applications: base editing, CRISPR activation and inhibition, among others. Over the past few...combination of MS2, PP7, or boxB sequences in the same sgRNA will generate additional colors. Image from...

  12. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...workshop took place in Boston - you can revisit the activities of our attendees there online with #comscicon18...suggests a change to a slide, think about whether the same principle applies to other slides in your deck.  ...

  13. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...brain, mapping neuronal networks, and selectively activating signaling pathways. Many scientists came to the...While walking around the exhibition area, I got a sampling of the broad variety of tools in neuroscience ...

  14. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...CpG islands makes  genes less transcriptionally active. The role of epigenetics in studying disease Epigenetic...both involve the same region of chromosome 15. They both result from the inactivation of genes on one ...corresponding gene products due to the same region, but not the same exact genes, being improperly silenced.... This is how most cells in the body contain the same DNA but have very different structure and function... genes from both copies of chromosome 15 are inactivated, but different phenotypes result depending upon...suppressor genes can repress their transcription and inactivate them. Global hypomethylation, on the other hand...are known to be involved in repression and X-inactivation. The K9 reporter uses the HP1 chromodomain to...

  15. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...plasmid will get diluted faster when cells are actively dividing. The typical drawbacks of using transfection...of time than traditional plasmids due to lower activation of DNA-silencing mechanisms. While minicircles...integration Insertional mutagenesis, transgene reactivation, residual expression Lentivirus Integrating...silencing of transgenes is a concern as well, and reactivation of Myc or other oncogenes after differentiation.... This can complicate comparisons made between samples, i.e. transgene expression and hence the extent...Cre-lox lentiviral vectors: This approach is the same as using a standard lentiviral vector, but transient...

  17. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...Central PMCID: PMC413431. 2. Bukrinsky MI, et al. “Active nuclear import of human immunodeficiency virus ...designed to make you sick…well, you feel it. But the same features that make these familiar viruses such a...a DNA/RNA cargo into their targets makes them attractive and tractable tools for genome engineering. Scientists... into an organism. This feature makes them an attractive tool for human subject delivery, as immunogenicity...

  18. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...now routine, open-source software projects that actively engage with the community are particularly successful...very difficult to adapt the device to one's own samples and limits the use of microscopy in open science...

  19. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...the provirus). Contains sequences necessary for activation of viral genomic RNA transcription. R; Repeat...While both lentiviruses and γ-retroviruses use the same gene products for packaging (Gag, Pol, and Env),...produce additional virus. Are γ-retroviruses self-inactivating (SIN)? They can be. Plasmids that encode SIN...

  20. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...HDR may inadvertently re-create a match for the active guide. With these constraints in mind, we implemented...scientists design and analyze batches of CRISPR samples. We invite you to jump in and try it out, or take... databases, including primer3, biopython, bwa, samtools, bowtie, UCSC genome browser, Ensembl, and others...currently Crispor and Crispresso, that adhere to the same principles listed here. It employs biological best...productivity in the Biohub. We hope it will do the same for you. Send us your questions or comments to gdingle...
Showing: 221 - 240 of 353 results