...connectivity to other data sources used in research; (2) connectivity to other tools used in research; and...by making it easy to get data out of the ELN; and (2) facilitate inter-operability with other research ...Github and Gitlab, and science-specific tools, e.g. sample management apps, colony management software, and..., not all of them support reproducibility in the same way. When choosing an ELN for your research, be ...commercialization community in greater Boston, while at the same time keeping one foot on the other side of the pond...

...estimate transformation efficiency (Figure 2).    Figure 2: Example of a serial dilution to estimate...library from Addgene, they’ll need to amplify the sample for further downstream applications. As individual... all of the bacteria can grow and compete in the same container, making it easier for a smaller, recombined...

...nonsense-mediated decay. Although Nelson et al. observed only 2% genome editing in one experiment, they found the ... cells throughout the body, especially stem cells 2. Remove any immunogenicity of the AAV vector Once ...Science. 2015 Dec 31. doi:10.1126/science.aae0169 2. Long C, Amoasii L, Mireault AA, McAnally JR, Li H...slightly different methods, they each observed the same positive phenotypes in cardiac and skeletal muscle...

...form or as ready-to-use lentivirus. Figure 2: dCas9-Tet1 demethylates cytosines at promoters and...pX330a dCas9-LSD1 for targeted removal of H3K4me1/2 and H3K9me2 histone modifications. Like the TALE-LSD1...approach for targeted DNA demethylation. Cell Discovery 2: . https://doi.org/10.1038/celldisc.2016.9 Find plasmids...activate transcription. A lentiviral vector with the same modifier, Fuw-dCas9-Tet1CD, is available from Rudolf...

...residues, which can be found throughout the antibody. 2) Endogenous cysteines, such as those that make up ...Detecting antigens by quantitative immuno-PCR. Nat Protoc 2:1918–1930. https://doi.org/10.1038/nprot.2007.267 ... antibody will have been verified to perform the same as its unconjugated version, but if you find yourself...

...et al., 2020). Genomic targets require an upstream 2-bp PAM sequence for recognition by QCascade; although...cells of the plasmid after integration. Figure 2: (A) Streamlined VchINT expression constructs. (B)...seven required genes and the CRISPR RNA under the same promoter, and a donor plasmid (pDonor) encodes the...immunity, whereby multiple proximal insertions with the same transposon exhibit reduced efficiencies (Vo et al...

...retain their individual activities when separated (2), and that DBD and TAs from different systems could... Enzymol. 1983;100:293-308. PubMed PMID: 6312261. 2. Keegan, L., Gill, G., and Ptashne, M. Separation ...libraries of bait and prey against each other at the same time in order to try and access the entire interactome...the protein partner or the UAS/reporter gene, the same bait and prey libraries can be screened using both...

...with neuron-specific expression of dCas9-VPR (Figure 2), a popular Cas9 activator developed by the Church...ubiquitous ones such as EF-1α and CAG. Figure 2: Dual lentivirus system with ubiquitous expression... Gene isoforms refer to mRNAs generated from the same gene but with different untranslated or coding regions...transcriptional start sites, all of which yield the same BDNF protein. To demonstrate the specificity that...

... pCRISPomyces-2 contains Cas9 and a gRNA cassette. The simpler system of pCRISPomyces-2 displays a higher...and a subsequent curing protocol that requires only 2-3 hours incubation. E. coli and T. citrea scarless...four major components including (1) a CRISPR RNA, (2) four proteins forming the QCascade DNA-targeting ...https://doi.org/10.1038/nbt.3043 Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA (2013) Programmable...

...different activators exist, including the multi-plasmid SAM system. Select Expression System To use CRISPR, you...either (1) a lack of gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells expressing...efficacious than gRNAs containing a C nucleotide at the same position in spite of being a perfect match for the...

...blood are reprogrammed immediately after collection [2]. PBMCs display unique epigenetic signatures that ...PMID: 27882265. PubMed Central PMCID: PMC5118044. 2. Kim, Y., et al., The Generation of Human Induced ...blood sample, in the best-case scenario [7]. In the clinical setting where patient blood samples are limited...grade hiPSCs from easily accessible patient blood samples. All pCXLE vectors listed below in Table 1 are ...

...days before the deadline isn’t going to be much use. 2. Originality is essential for grant success The two... it’s important not to throw in the towel if 1 or 2 funding bodies say no. If possible, get feedback from...institution). Likewise, if you’re staying in the same lab where you have been working for the last few...

...from animals or hybridomas.   Figure 2: (Left) Figure 2: (Left) The light chain and heavy chain genes...antibody expression. If starting from a protein sample (for instance, affinity purified antibodies from...

...is funded have been well documented over the past 2 years (Alberts et al, PNAS 2014; Taylor, eLife, 2016...PMID: 24733905. Pubmed Central PMCID: PMC4000813. 2. Taylor, Justin Q., et al. “Avoiding a lost generation...postdocs at institutions, will be challenging. At the same time, there is much opportunity to make headway ...

... both substantial merit and national importance; (2) The foreign national must be well positioned to advance...outstanding achievements in your specific academic field, (2) that you have three 3 years of research experience..., Casseus Law suggests a mix of experts including 2 dependents –people who have worked with you i.e. your...working in October of the same year. The Industry H-1B visa is not the same as an academic H-1B visa and...individually, as a judge of the work of others in the same or allied academic field Evidence of original scientific...

...everything but the CD8+ T cells (Figure 2). Even better, we can use the same fluorophore for the three lineage...     Figure 2: Dump gating labels all unwanted cell lineages with the same fluorophore to easily...FACS achieves greater purity of your sorted cell sample than MACS. An effective strategy to sort out your...different while the permeabilization solution can be the same). Examples of such targets include transcription...undesired cell populations. Dump gating uses the same color for different markers, making it easy to gate...

...required components are 1) a SMASh-tagged protein and 2) asunaprevir (small molecule). dTAG The degradation...gone through an additional round of evolution (AID-2) to reduce some of the leakiness associated with the...the general concept of the system has remained the same. An obvious drawback of the system is that it requires...

...engineering methods have their own pros and cons (2). The NgAgo DNA guide is inexpensive and can be 5’...Nature biotechnology (2016). PubMed PMID: 27136078. 2. Burgess, Shawn., et al. "Questions about NgAgo." ... to deliver NgAgo ingredients into the cell, the same method used by Gao et al, 2016 (1). View the Complete...

...from GFP and the mutation definitions found in Table 2 may not apply. Table 1: The specific mutations comprising..., V163A EBFP F64L, S65T, Y66H, Y145F   Table 2: Functional role of specific mutations in GFP derivatives...that of the gene of interest, or expressed from the same transcript as the gene of interest, but after an...

...the chromosome, ending replication.   Figure 2: Prokaryotic chromosomal replication. Adapted from...Microbiology and Molecular Biology Reviews: MMBR, 62(2), 434–464. https://doi.org/10.1128/MMBR.62.2.434-464.1998...machinery in order to replicate—but not always to the same extent. As described in our previous Origin of Replication...proteins, it can be regulated indirectly via the same mechanisms that affect DnaA and IHF. RepA also controls...