...contain various promoters and reporters, and subsequently inserted into a Cas9-containing destination ...inserted. The BsaI site remains intact, allowing subsequent “bricks” to be added one by one. This approach...time needed for a single round of editing, and a subsequent curing protocol that requires only 2-3 hours ...

...eluting the DNA from the substrate. In a column-based approach, the substrate is a silica matrix fixed...the plasmid DNA from your cleared lysate to a substrate, washing away left-over debris that carried over...

...phosphatase treatment, substituting cytidine with 5-methylcytidine, and/or substituting uridine with pseudouridine...selection is removed. Yu et al found that ⅔ of subclones generated from EBNA1 iPSCs still had episome DNA...

...four! (Note: For cases where the same name was submitted by more than one person, we selected the finalist...finalist based on the first time of submission.)     We love the way the names Aavery, Addy, and Cappy...

...over, we will host other contests in the future. Subscribe to the blog to stay in the know!     No pranks...see a reference to this scavenger hunt and the subsequent clue. (Begin with clue 1 below!) You will find...

...fantastic team of high school students who recently submitted to the journal with funding from Addgene. Listen...personal networking!) and the manuscript review/submission process at JEI. Chapter 3 - JEI target audience...

... last week perusing the Deck The Lab contest submissions on Twitter and Instagram and we’re excited to... 17, 2019   Lab material tree We often get submissions of decorations created from lab materials. This...

...Fluorescent Protein Plasmids 101: Visualizing Subcellular Structures and Organelles Resources at Addgene.org...Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...

...entry will reveal additional data including the subject’s age at the time of injection and how long the ...contributed data so far and we encourage you to submit data from your experiments. You will be acknowledged...

... critical systems that cannot be removed or substantially reduced in an attempt to save energy. The transition....  Additional resources on the Addgene blog Subscribe to the Addgene blog Read our sustainability in...

...you time and improve your experiment results! Subscribe to the Addgene YouTube page! AAV purification ...lab tips, and more video content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources...

... Addgene office as we peruse the #DeckTheLab submissions. We asked all of the Addgenies to pick their ...Nika Heijmans There were many science-themed submissions: string lights hung up in the shape of RNA, light-up...

...hypothesis was that trained muscle would produce a substance with beneficial effects on the brain,” Ruas said...produces an enzyme that purges the body of harmful substances. So in this context the muscle's function is ...

... A KTR is comprised of a fluorescently-tagged substrate for a kinase of interest which is further fused... used to measure kinase activity in specific subcellular compartments. Second, because KTRs shuttle between...

...Experiments by Jennifer Tsang Chromoproteins are a subset of the fluorescent protein family and they generate...molecular biology tools out there! Here, we highlight a subset of these tools for plasmid mapping, DNA/protein...

...targeting the mouse and human kinomes. While any subset of the genome is limited by definition, the kinome...plasmids contain the same 3-4 gRNAs found in the first subset of the pooled library. The individual plasmids ...

...a few species of bacteria and sometimes, only a subset of strains of the same bacterial species are affected...developments at Phage Directory and in the phage field, subscribe to their newsletter, Capsid and Tail. Additional...

...once science was only seen as a serious academic subject, it is now being appreciated on an aesthetic level...which could promote conservation efforts, as the subject matter is seen to have increased value and is appreciated...

...locus may be useful to study haploinsufficiency. Subsequent modifications at the remaining allele within ... Lin C, et al. An erythroid enhancer of BCL11A subject to genetic variation determines fetal hemoglobin...

...expectations were for the intended position. After submitting my application, I was contacted to participate...discrepancies between what we sequenced in house and the submitted sequences were a concern to the plasmid function...