We narrowed to 447 results for: SUP

1. Transferable Skills Guide: Identifying Your Transferable Skills

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   Blog Post

   ...it just as much in my current role as Customer Support and Operations Manager at Addgene. In this role...The Muse. Kai Strickland is a former Customer Support and Operations Manager at Addgene and is interested...

2. AAVs CREATed for Gene Delivery to the CNS and PNS

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   Blog Post

   ...the CNS (figure 5 in Chan et al) and the PNS (supplemental figure 8 of Chan et al). These new AAVs can ... transgenic mouse required (see figure 5 and supplemental figure 8 in Chan et al).  References 1. Chan...

3. Negative Can Be Positive: Open AAV Data with Addgene

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   Blog Post

   ... was far from an isolated case. Our scientific support team answers hundreds of AAV questions every year...Figure 2.) At the same time, when scientists who support open science, but do not share their own data -...

4. CRISPR Antimicrobials

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   Blog Post

   ... to eliminate the use of antibiotics by their suppliers, and public health and medical organizations are...win back ground in the fight against bacterial superbugs. References 1. Citorik, Robert J., Mimee, Mark...

5. Twenty Years of Sharing: Addgene's Viral Vector Service

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   Blog Post

   ...vectors not only available, but accessible. The supporting data and community engagement required to do ...lessons learned from this process have shaped and supported our growth and development. We’ve shared what ...

6. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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   Blog Post

   ...variety of cellular processes and an antitoxin that suppresses the toxin’s activity. The key to these systems...long-living toxin that damages cell membranes; Suppression of killing (sok), a short-lived RNA antitoxin...

7. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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   Blog Post

   ...vector toolkit It has been shown previously that suppression of TP53 enhances iPSC generation as does replacing...Central PMCID: PMC3222670. 10. Hong, H., et al., Suppression of induced pluripotent stem cell generation by...

8. CRISPR-based Genome Editing Systems For Editing in E. coli

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   Blog Post

   ...genomic site of interest. The donor template is supplied separately. To use this system, you’d first introduce...with the original system, the pEcCas/pEcgRNA is superior in several ways: The pEcCas plasmid can be operated...

9. Transferable Skills Guide: Creativity

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   Blog Post

   ...be resistant to change. Ask your peers or your superiors to explain their rationale when they’re resistant... currently plaguing the scientific community is supply chain shortages. When flasks, conical tubes or ...

10. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    Blog Post

    ...). Neurology 92 (15 Supplement) . http://n.neurology.org/content/92/15_Supplement/S51.002.abstract Sinnamon...

11. Hot Plasmids: Summer 2024

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    Blog Post

    ...purification and minimizes sample loss while also supporting imaging, making it easy to identify dozens or...hybridoma-derived parent antibody (N206A/8 TC Supernatant). Image attribution: James Trimmer, UC Davis....

12. The Fluorescent Vegetables in Aptamer Soup

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    Blog Post

    ...photostable, and were presented in the study to support the uniqueness of the photostability of the Corn-DFHO... Matthew D. Disney, and Samie R. Jaffrey. "A superfolding Spinach2 reveals the dynamic nature of trinucleotide...

13. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post

    ...mutations are introduced, and scalability (see Supplementary Table 1 of Wrenbeck et al). It uses a uracil...about the same number of positive clones. See Supplementary Note 1 in Wrenbeck et al for details on how ...

14. 10 Steps to a Perfect Science Talk

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    Blog Post

    ...you rather hear it from your peers than a less supportive audience? 7. Hone Your slides Use big images ...’s attention will start to wander when you are supposed to be done. They will start looking at their phones...

15. Technologies Enabled by NanoLuc® Luciferase

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    Blog Post

    ...share your constructs from lab to lab. Promega supports the Addgene mission statement: Accelerate research...for product development and now specializes in supporting Promega cellular analysis products at Promega...

16. Finding Your Perfect Job After University

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    Blog Post

    ...thesis on the study of e2Fb, which is a tumour suppressor. This summer role was incredibly valuable because... and interests. I was so glad when my future supervisors were keen for me to become involved with many...

17. 9 tips for a successful postdoctoral experience

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    Blog Post

    ...are your support network, so remember to thank them verbally and often for their love and support. Many...

18. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    Blog Post

    ...environment, they are difficult to eradicate. You can suppress the growth of mycoplasma but cannot eradicate ...for detecting mycoplasmas. In this method, the supernatant from the cell culture is added to the liquid ...

19. Finding Your Science Policy Path

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    Blog Post

    ...pursue your interests in policy or advocacy as a supplement to your career through engagement with your local...have opportunities to join commissions or boards, support ballot initiatives, deliver public comments, meet...

20. How to Deposit Your Plasmids with Addgene

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    Blog Post

    ...the gene/insert. Pooled libraries are normally supplied as liquid DNA with all of the plasmids in the ...be entirely verified by sequencing. Upload any support files that will make it easier for scientists to...