We narrowed to 931 results for: TIM;

...Years later, these classic systems continue to be optimized by Tabor’s lab, making light-controlled gene expression... biological processes. Chemical effectors are suboptimal for a number of reasons, including their potential...high or very low levels. Schmidl et al. chose to optimize these systems to make them more tunable and user-friendly...transcription factors, for which low expression levels are optimal. These tools are compatible with many strains of...et al. also represents a large step forward in optimizing TCSs. Over 75,000 TCSs have been identified in... in bacteria, and with similar optimization methods, many of these systems could be made suitable for ... applications.   References Refactoring and optimization of light-switchable Escherichia coli two-component...

... available amino acids a bit…limiting. It may be time, then, to look towards genetic code expansion (GCE... which non-canonical amino acids (ncAAs, also sometimes called unnatural amino acids) are placed in a ...along with the inherent trickiness of using and optimizing GCE, led to a large toolbox filled with systems...dubious enough that they don’t give it a try. Optimization Why is GCE so complicated? Well, while the techniques...growth temperature) may need to be individually optimized for each of the three factors: the ncAA, the tRNA... synthetases. And, naturally, rarely are the optimization parameters the same for all three components...including their phosphoserine ncAA protocol, to optimize and share, they’re soliciting feedback from scientists...

...that the optimal buffer for Taq DNA Ligase contains 100 – 200 mM KCl at pH 8.5. This optimization method...condition optimization, will be critical for success. Online tools are available to help optimize ligation... the incidence of annealed mismatched dsDNA. Optimization through high-throughput profiling New England... both the enzyme and the reaction buffer were optimized, resulting in the highest fidelity NAD+-dependent...Reaction Temperature Calculator can be used to estimate an appropriate incubation temperature when using...: 23842410. 19. LeClair, Norbert P., et al. "Optimization of a ligase detection reaction-fluorescent microsphere...

...platforms like HaloTag® Fusion Protein, codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc...issues as traditional luciferases emit at near optimal wavelengths (Fluc emits at 610 nm) and do not require...’ dim light emissions requires long acquisition times that may be impractical or infeasible for certain... emission peak is at 460 nm thus making it non-optimal for in vivo imaging. The solution is to couple ...quantum yield, brightness and improved maturation time compared to similar orange fluorescent proteins....et al. (5) imaging multiple proteins at the same time in live cells using fluorescent proteins (FPs) can... variants of bright luminescent protein for real-time multicolour bioimaging. Nature Comm. 7, 13718. PubMed...

...combined optical and genetic methods to photo-stimulate mammalian neurons and alter synaptic events: a...rhodopsins and neurons have in common? Neuronal stimulations are triggered by the movement of ions across...viral-infected neurons are subsequently photo-stimulated through fiberoptic cannulas directly implanted.... In particular, they found that optogenetic stimulations of the DA neurons produce behavioral and electroencephalography...could activate appetitive rather than aversive stimuli. In a recent paper, de Jong and colleagues used...neurons of the VTA. Using in vivo optogenetic stimulations, they simultaneously recorded the electrical...PMC3225502. Boyden, Edward S., et al. "Millisecond-timescale, genetically targeted optical control of neural...

... a full-time career or a part-time passion, there are many ways to make it work for your time and interests...is science policy?”  I have been asked this many times throughout my short science policy career and this...including fellowships, volunteer positions, and part-time opportunities. But even through all my roles and...academia as tenured professors. There’s never a bad time to start your science policy journey.  In fact, ...Note: the answer to this question may change over time and that’s perfectly normal!  Action Items:  Start...presenting for non-scientist audiences during your time in graduate school through outreach organizations...

...this really sounds like a two-way ANOVA. And this time, it is! Factorial ANOVAs include two-way ANOVAs ...impact of temperature, humidity, sunlight exposure time, and watering volume on total crop production (though...the only things that matter. However, this is oftentimes an oversimplification. If your independent variables...every pairwise comparison within your population? Sometimes this can’t be avoided, but if it can be, you can...example, if your alpha is set at .05, then 5% of the time the statistical test will be incorrect. If you have...high false positives. Control vs. all samples Oftentimes there exists a control group and multiple experimental...working with, graphing data with a histogram is sometimes sufficient to determine normality (or lack thereof...

...Before diving into the experimental work, spend some time outlining the construction of the plasmid and all.... It is always a good idea to perform primer optimization, especially if you are having difficulty amplifying... genome, you can recheck your primer design or optimize your primers or PCR conditions. Purifying DNA...increase your chances to have a successful and ultimately faster cloning. Remember to quantify the DNA ... parameters that you can adjust: temperature and time of incubation, and amount of DNA. As a general rule...plasmid:insert), but this parameter has to be optimized according to the strategy adopted, and to the ...and if the strain is growing fine, it is probably time to prepare new plates with a fresh stock of antibiotic...

... and significant lengths of time, the stability of fluorescence over time is crucial. For instance, the... century, it can be overwhelming to select the optimal dye or fluorescent ligand when designing an experiment...intensities, and the best practice to determine optimal DOL is through several small-batch experimental...can be mitigated through careful selection and optimization. Fluorescent experiment types: Different types...super-resolution microscopy techniques, such as Stimulated Emission Depletion (STED), and Stochastic Optical...non-fluorescent states in response to a specific stimuli, which leaves only a small volume of fluorophores...technically cell-permeable, its use in live cells can sometimes result in cellular toxicity due to the permeation...

... do you have to work with difficult people? Each time I ask this question at my workshops, I get nods ...impolite, Complainers, who spend most of their time moaning about their troubles, Silent or unresponsive...supervisors: Hands-off super busy types, who never have time to mentor you, and Micromanagers, who question.... You can save yourself and your supervisor some time if you come to every meeting with one or more proposals...Your supervisor will appreciate that you took the time to think of solutions, and will be more likely to...your action item list and set some approximate timelines. Be sure that you keep your commitments, and if...your point across. Some supervisors might try to intimidate you with negative comments (hostile-aggressives...

...conversation to pass the time in a job that wasn’t going anywhere, but it became a legitimate brain exercise in...still flip to that page in my lab notebook from time to time. I wrote in large letters “Going for it!” We...there very few jobs available. So, we spent a lot of time talking about what we could do to make ourselves...weren’t restricted to science jobs. Keira’s an optimist, I’m a realist, and together we make a pretty ... 2014. Outside of the lab, Keira enjoys spending time with her husband and two Jack Russell Terriers. ...

...on the Addgene Blog Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell...colonies of antibiotic-resistant cells; during the time required to grease, place and fill a dozen cloning...dilution cloning into 96-well plates, is extremely time- and resource-consumptive, since only wells with...well-spaced clones. For rapidly growing cells we sometimes use TPP peelaway T-75 flasks to provide a greater... in outgrowth. Depending on the cell type, we sometimes add a drop of serum to each well in the 48-well... the University of Maryland Medical School in Baltimore. She is particularly interested in signaling peptide...

...applications (qPCR and ddPCR.) qPCR Quantitative/real-time PCR (qPCR) has been used for copy number assays ...or negative for each reaction, math happens (this time, Poisson statistics), and the readout gives an absolute...Gommersall L, Arya N, Patel HR. Basic principles of real-time quantitative PCR. Expert Rev Mol Diagn. 2005 Mar...Quantitative analysis of copy number variants based on real-time LightCycler PCR. Curr Protoc Hum Genet. 2014;80:...hg0721s80 Colborn JM, Byrd BD, Koita OA, Krogstad DJ. Estimation of copy number using SYBR Green: confounding ...Na-Bangchang K. SYBR Green I and TaqMan quantitative real-time polymerase chain reaction methods for the determination...determined by SYBR-Green-based quantitative real-time PCR. J Microbiol Methods. 2006 Jun;65(3):476-87....

...articles.  Here's what you'll find in this post: Optimized prime editing in dicots Self-labeling tags for...Antibodies for neurodegeneration research   Optimized prime editing in dicots By Emily P. Bentley Prime...combining several approaches (Vu et al., 2024). Their optimized strategy uses new combinations of PE protein components...recombined prime editing tools PE2max-NC and ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT...Song, Y. J., Nguyen, T. H., & Kim, J.-Y. (2024). Optimized dicot prime editing enables heritable desired ... their propagation along dendrites for the first time (Figure 3). The team also used AAV9 to deliver ASAP5...expresses all the components of the biosensor in optimal proportions from a vector backbone that allows ...

...interview requests and visitation days can be, at times, very overwhelming. As someone who applied to PhD...interviews, so check if yours does and then use that time! Different platforms have different quirks, so remember...department worked with students to maintain these time goals during COVID-19? How are students funded? ... have questions about the program and having the time to ask personalized questions might be difficult...your desired program will allow for you to have the time and space to delve into what matters to you. It ...worry about traveling during an already stressful time, and I was more relaxed during both sets of interviews...interviewing allowed the interviews to happen even in times of great uncertainty. Having virtual and hybrid ...

...languages? That's trickier. Sometimes it's the developers. But, it turns out over time that the most successful...use plasmids, they are trying to do one thing at a time. What the BioBricks Foundation is seeking to accomplish... up, and slam that onto a mass spec. That's very time intensive and expensive. There's the promise that...seen the development of enough sensor domains. Ultimately, we'll need a couple thousand or so sensor domains...bioeconomy is already pretty substantial. It's estimated that genetic engineering as applied to products...want is 93 percent. All of a sudden, for the first time, you've got a collection of reusable, off-the-shelf...

...some genetic diseases. I didn’t know it at the time, but gene therapy had fallen - plummeted really ... organ that can be monitored non-invasively over time. Importantly, it is an immune privileged compartment...destructive inflammatory immune response. At the time, adenoviral vectors were the most commonly used ...cells - AAV - were becoming available. At the same time advances in human genetics led to the identification...brought all these key elements together at the right time and place. But it is the hard work and persistence...deliver via AAV.  Dual vectors strategies are being optimized to overcome this limitation, with limited success...designing expression cassettes to regulate and optimize transgene levels, and large scale manufacturing...

...perform. It required a great deal of time and effort to test and optimize our vector - overall, I spent two...within the same brain region or nucleus. Just optimizing AAV expression can take months (or longer), yet...testing and reformulating our AAV construct to optimize performance in our system and yet none of that...to see more negative data (Figure 2.) At the same time, when scientists who support open science, but do... C., Garner, K. M., & Adan, R. A. H. (2010). Optimization of Adeno-Associated Viral Vector-Mediated Gene...Researcher Perspectives on Data Sharing in Deep Brain Stimulation. Frontiers in Human Neuroscience, 14(December...

...slog through unappealing work in your precious free time.  And don’t be afraid to think outside of the box...project, I can guarantee you that either resource or time restrictions (or both!) will be a major limiting...understand what kind of resources - including your time - will be required to produce an outcome you’re ...proficiency.  What kind of content are you sharing?  Sometimes your subject matter will drive the kinds of communications...presentations, etc… Low (except for space) Real-time audience engagement and response. High connection...resource geographic location) Opportunity for real time engagement while allowing for recording/future access... speed internet access. High probability of real-time technical difficulties Exhibits Medium to high...

...can be shortened since the plated cells will have time to produce beta-lactamase prior to division, instead...effects of the antibiotic. This buys you a little more time, since bacteria won’t divide immediately after it...beta-lactamase. Pros: Widely available, cost-effective, timesaving for transformations. Cons: Prone to satellite... Pros: Very stable, interchangeable with Amp, timesaving for transformations. Con: Expensive.   Kanamycin...resistance Choosing the best antibiotic resistance ultimately comes down to your applications and needs. The...selecting!   Antibiotic Resistance Stability Time Saving?  Works for multiple organisms? Compatible...storage temperature and pH on the stability of antimicrobial agents in MIC trays. Journal of Clinical Microbiology...