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We narrowed to 1,075 results for: TOR

Showing: 221 - 240 of 1075 results

  1. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...fusion to a receptor-transcription factor fusion. TEV protease cleave the transcription factor from the ... of two different receptors- angiotensin II type 1 receptor and the β2 adrenoceptor. GPCRs in chemogenetics...down the name "G-protein coupled receptor" into two parts: the receptor itself and the G-proteins it associates...associates with.  Receptor: GPCRs are also known as seven-(pass)-transmembrane domain receptors, due to the ...pharmacologically-inert, small molecules. These new receptors were termed Designer Receptor Exclusively Activated by Designer...of the five senses? GPCRs or G-protein-coupled receptors (GPCRs)! Aside from these three senses, GPCRs ...in inflammation, and neurotransmission. These receptors can be activated by an array of different ligands...

  2. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...twenty years at Addgene, we are sharing stories of our repository’s positive impact on science and scientists...three functional groups: nucleases, modulators, and chimeric effectors. Nucleases generate a classic double-stranded... distributed CRISPR plasmids. But modulators and chimeric effectors, made available in 2013 and 2016, ...community better. We therefore looked at depositors and requestors by country, and graphed the top ten countries...no surprise that the USA is the largest depositor and requestor of CRISPR plasmids. But many other countries...from Japan to Australia, are significant depositors and requestors. CRISPR tools are developed and used all...think so — and so do our requestors! CRISPR plasmids in the Addgene repository have been cited a total ...

  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...cytidine deaminase portion of the base editor to create SpCas9 base editors with editing windows as small as...some phages fight back using CRISPR inhibitors. Several such inhibitors have been characterized for type ...bacterial and mammalian expression vectors for these CRISPR inhibitors are listed in the table below. ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief...posts for every great plasmid that comes into the repository, but be sure to let us know if you'd like to ...TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that have standard cloning sites or gateway compatible...cell types directly linked to disease, including motor neurons (-3mnx1/Hb9), pan neuronal (elavl3/HuC),...

  4. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...plasmids available from this depositor's lab. Plasmid Description Gene/Insert Vector Type PI Publication Back...available plasmids from this depositor's lab. Plasmid Description Vector Type Selectable Marker PI Publication...Construction Integration Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the...Back to Top Shuttle Vectors Search the table by keyword or sort by the table headings. Click on the publication...

  5. NETRF

    Type
    Collection
    ...from the NETRF-funded investigators listed below. Depositing Principal Investigators (PIs) PI Institution...Addgene Depositors that have received funding from the Neuroendocrine Tumor Research Foundation Depositor...Shivdasani Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...Tumors Qiao Zhou Harvard University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...

  6. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...into distinct expression vectors using the enzyme BbsI. These expression vectors each contain Type IIS restriction...Cas9 or dCas9-containing destination vectors. Destination vector options include humanized wt Cas9, dCas9...-VP64 (transcriptional activator)-containing plasmids. Each destination vector contains GFP, enabling ...create lentiviral vectors expressing wt humanized Cas9 and up to three gRNAs. Entry vectors containing the...wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors containing other...containing vector using Golden Gate assembly, and up to 8 gRNAs may be expressed simultaneously. Vectors for ...Joachim, et al. “A versatile modular vector system for rapid combinatorial mammalian genetics.” Journal of ...

  7. Viral Production at Addgene

    Type
    Blog Post
    ... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles...vivo studies. Preparations are then aliquoted and stored at -80 °C. Titer Titering is either performed by...to determine which facility produced your viral vector lot. At Addgene, AAV particles are titered by droplet...method.  Titering by the University of Pennsylvania Vector Core is (as of April 2016) also performed by ddPCR...

  8. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...students, and those from historically underrepresented groups. Through such mentoring programs, scientists...especially grateful to its mentors and supporters during this first year. Mentors Amanda Gunn, Bryan Gibb.... The teaching/mentoring bug never went away. While he has frequent bouts with Impostor Syndrome, Joe ... done at my first six ASM meetings. I told this story over a great dinner at Pittypat’s Porch with old...James et al., 2020. Help me stranger - Finding mentors for the meeting Just like analysis pipelines for... meetings. A key was to provide each bin with a mentor who had attended the meeting multiple times before...plan for and network at the meeting. We found our mentors by asking for help with the program on social media...

  9. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...community concerns AAV vectors are complex biological reagents; many factors, like storage buffers and conditions...when I started working with AAV vectors. I wanted to use an AAV vector to express ChR2(H134R) in A2 norepinephrine...to AAV vectors, many from people trying to answer the exact same question I was: What AAV vector is best...Working with AAV vectors in the brain and peripheral nervous system presents a special challenge to scientists...question and used it to publish!  Working with AAV vectors in neuroscience Let’s consider my first challenge...didn’t provide any information on other important factors such as what is the best promoter to use, or if...deal of time and effort to test and optimize our vector - overall, I spent two years testing and reformulating...

  10. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...Choose a moderator with a backbone. It is nice to have a subject area expert, but the moderator is not ...LinkedIn profiles of all panelists to the moderator. The moderator and organizers should develop a list of...interviews, the moderator should be taking good notes and discovering interesting stories, riffs or opinions...contribute. The moderator should identify the funny, provocative or interesting stories that will make ...bring out these interesting stories and remind the panelists of the stories or topics you know they can...(lapel mics are best). The moderator takes control It is the moderator's job to prevent anyone from ...published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates. Panel discussions are becoming...

  11. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...designed their AAV tagging vectors (pAAV-SEPT-Acceptor and pAAV-TK-Acceptor) so that they can be easily...gene. pAAV-SEPT-Acceptor, the preferred vector, contains a promoterless splice acceptor-IRES-neoR gene,...cloning them sequentially into the chosen acceptor vector. The vectors are then packaged into AAV virions via...identical targeting vectors delivered through transfection of naked DNA. Other investigators who had been working...Check out our Viral Service Find AAV Vectors Browse all Viral Vectors ...well understood, researchers have been using AAV vectors for gene editing in mammalian cells since 1998....when delivered via AAV infection, gene targeting vectors with relatively short (1 kb) homology arms underwent...

  12. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...of the most compact genome editors to date. We have developed multiple vector versions for TnpB-mediated...especially valuable for regulatory approval and public acceptance. Recently, viral vectors have been successfully...Practical Guide: Using pK-TnpB1 and pK-TnpB2 Vectors The vectors pK-TnpB1 and pK-TnpB2 are designed for plant...the digested vector. Step 4: Ligation Ligate the annealed oligos into the digested vector using your ...Precise plant genome editing using base editors and prime editors. Nature Plants, 7(9), 1166–1187. https...blog post was written by Dr. Kutubuddin Molla, investigator at ICAR-Central National Rice Research Institute...efficient delivery into cells, particularly via viral vectors. While viral delivery is a highly effective method...

  13. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ..., Word docs, and the like is likely stored in online file storage tools like Dropbox, Box, One Drive, ...commonly used online file storage tools, as illustrated in this video, and to data stored on institutional and...source data repositories Over the past decade or so a number of general purpose repositories have been ... in the repository after the deposit has been made. It’s possible to associate the depositor’s ORCID ID...value the repository integrations, because when RSpace documents are deposited into the repositories, links...research; and (3) connectivity to open source data repositories. To illustrate this, let’s take a look at the...researchers and PIs, science librarians and data curators, IT managers, the commercialization office, the...

  14. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ... Depositor Collections Botman-Teusink Yeast FP Collection...Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with ...yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al...overexpression of codon-optimized FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007...blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902. https...Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection markers for engineering...

  15. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...HiBiT-TP53 Fusion Vector at Addgene! Following extracellular RNA editors Tracking factors that utilize exosomal... to track epidermal growth factor receptor (EGFR), a transmembrane receptor tyrosine kinase essential ...EGFR-HiBiT Fusion Vectors at Addgene! Measuring messengers: growth factors Growth factors present unique ...measurements. Find CFTR-HiBiT Fusion Vector at Addgene! Real-time tracking of receptor internalization Work conducted...This method offered kinetic, non-invasive monitoring of receptor dynamics at the cell surface. Find HiBiT-EGFR...interactions with co-receptors (like NRP1) and by endocytic trafficking of the receptor. Peach et al. (2021...to subtle changes in co-receptor dynamics, including NanoBRET assays to monitor the binding of fluorescently-labeled...

  16. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...communication. Viral vectors and the nervous system With the advent of viral vector technology, new methods...been used for tracing and visualizing motor, visual, and auditory neuronal circuitry in vivo. A variant... at Addgene). AAV vectors: A platform for delivering neuronal tracers AAV vectors have been widely used...and Mooney, R. (2017). Identification of a motor to auditory pathway important for vocal learning. Nat ...genome is a single-stranded DNA molecule and AAV vectors are replication deficient (i.e. they cannot easily... low immunogenicity and cellular toxicity. AAV vectors can be equipped with strong promoters for high ... is often made Cre-dependent. If Cre-dependent vectors are used in transgenic animals that express Cre...

  17. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ... edit (or edits) engages the prime editor protein. The prime editor consists of a Cas9 nickase fused to...range of specialized prime editors (PEs). PE6a and PE6b are small prime editors with RT domains derived ...genome editing methods using nucleases and base editors have the potential to correct only a minority of...the edited strand. The first iteration of prime editor simply fused the wild-type Moloney Murine Leukemia.... We’ll summarize the major ones in Addgene’s repository later in the post. Advantages of prime editing... constrained by PAM sequence location The prime editor extends the reach of CRISPR genome editing, as ...either DNA strand, many previously developed base editors with a <8 base-pair editing window cannot edit ...

  18. Recombinases AAV Preps

    Type
    Collection
    ...plasmids in the repository. Browse our AAV collection of site specific recombinases.... Viral Vector Packaging ...Packaging Service AAV Recombinases Viral Vector Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible...Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . Don’t See What You’re Looking For? Our ... eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know...format. Please note this does not guarantee viral vector packaging service, but lets us know what viruses...

  19. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...ingredients: the lentiviral packaging vector, a transfer vector including the gene of interest, and the...3rd-generation transfer vectors apart? A3: Addgene defines 2nd- or 3rd-generation transfer vectors based on whether...between 2nd- and 3rd-generation vectors? What does this mean for transfer vectors? A4: The lentiviral packaging...largely based upon the vector system used and the transgene insert encoded by the vector." The table below ...additional information or clarification on these vectors. Read on to find the answers to our most frequently...questions. This useful resource from Addgene Depositor (and lentiviral expert) Didier Trono's lab webpage...years based on HIV-1; however, the 1st-generation vectors are never used as they pose too much of a biosafety...

  20. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...large size of Cas9 activators, which largely precludes delivery by adeno-associated vectors (AAV). This issue...Suntag” Cas9 activator that recruits up to twenty copies of the p65-HSF1 transcriptional activator to each ...found here. Neuron-optimized viral vectors Alternatively, Cas9 activators can be delivered to the CNS by ...mCherry (vector 1) with neuron-specific expression of dCas9-VPR (Figure 2), a popular Cas9 activator developed...Patrick Miller-Rhodes, a Ruth L. Kirschstein NRSA Predoctoral Fellow at University of Rochester Medical Center...including transgenic mice, neuron-optimized viral vectors, and high-throughput screening approaches. Here...and engineered combinations of transcriptional activators to induce gene transcription at genomic sites...
Showing: 221 - 240 of 1075 results