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... Kolmer-Agduhr cells was sufficient to trigger the symmetrical tail movement unique to spontaneous slow...periodically beat their tails left and right in a symmetrical motion to move forward. This behavior is not ...behavior studies in action: Identifying neurons that trigger spontaneous swimming  Researchers at the Isacoff... zebrafish larvae to identify new neurons that trigger the CPG (central pattern generator) (Wyart et al... photostimulation in order to drive the full symmetrical response.  The function of Kolmer-Agduhr cells...

...sequencing results match sequence information contributed by the depositor, published backbone sequences...additional verification experiments: Primer and restriction digest design Some regions of plasmids are difficult...present in the plasmid, we might need to perform a restriction digest to confirm the size of the plasmid and...including our most popular primers and certain restriction enzymes, but you will also be able to create ...annotations and design additional experiments like restriction digests. We’ll have a tutorial showing all of...Basic Molecular Biology Protocols Diagnostic Restriction Digest Protocol ...

...the list weekly. The Blue Flame Award, first distributed this year, serves to congratulate researchers...one plasmid deposited at Addgene that has been distributed more than 100 times. We hope these desktop trophies... or personnel to constantly prepare them for distribution. One of the major advantages to depositing materials...Laboratory in Norwich, UK: “We’re very pleased to distribute our plasmids via Addgene. Given the increasing...world. “It’s easy to imagine how hard it is to distribute thousands of plasmids across the world from the...has made it possible to eliminate geographical restrictions in life science research." Claim Your Award ...

... submissions via email: [email protected] Entries include accession number, species, and assigned...cell lines profiled in the lab. You can browse entries by cell line groups, such as their vaccine production...collections.  Depending on available information, entries may also include whether a cell line is known to...programatically, their API allows fine grained query and retrieval of all the information in the resource.  This ...cells. Image courtesy of Josef Reischig.  Contributing to Cellosaurus Generated a new cell? Or finding...sent the updated information to the cell line distributor. A success all around! If you’re excited about...

...pR6K ~15-20 R6K* C Stringent pACYC ~10 p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300...of replication is referred to as "relaxed" or "stringent" depending on whether the ori is positively regulated... do you choose? Addgene Senior Scientist Marcy Patrick says researchers can ask themselves a few questions...copy number of a plasmid, other external factors contribute as well. These considerations are especially ...are temperature sensitive; others ori's can be "tricked" into amplifying more copies with the addition ...

...resource of over 100 entries is driven by the dedicated community of data-contributing researchers who use...This word cloud (using wordart.com) represents contributed data on brain injection site from the AAV data...recently used Addgene AAV tools, we invite you to contribute your data to grow this resource for the community...opportunity to cite the image to give proper attribution.  We hope that this provides a clear overview...to all of those dedicated scientists who have contributed to the AAV Data Hub. If you haven’t yet, we encourage...

...transport at the cell surface Article contributed by Marcy Patrick  Listen to the nanobody toolkit podcast...highly processive reverse transcriptase Article contributed by Will Arnold  Listen to the MarathonRT podcast...occupying a specific genomic locus: Caspex  Article contributed by Susanna Bachle  Listen to the Caspex podcast...occupying a specific genomic locus: C-BERST Article contributed by Klaus Wanisch  Listen to the C-BERST podcast...dCas9–APEX2 biotinylation at genomic elements by restricted spatial tagging), to confirm the composition ...

... This post was contributed by guest blogger Courtney Price, the Education & Outreach Specialist for the...mission is to collect, preserve, reproduce and distribute seeds, DNA and other resources for Arabidopsis...100,000 samples to researchers and educators in 60 countries each year. Introducing Arabidopsis Arabidopsis...fluorescent lights. Other experiments require only seeds, petri dishes, and filter paper. Another benefit of using...learning. Whether visiting classrooms, hosting field trips, conducting teacher workshops, or staffing a booth...partnerships have grown to include local school districts, professional education associations, and local...

...This post was contributed by guest blogger Katrina Armstrong, a Neurophysiology Msc Student at the University...communities. Many thanks to our guest blogger Katrina Armstrong. Katrina Armstrong is a Neurophysiology Msc Student...better (3). Brain Region  Optimal Serotype Striatum AAV5>AAV9>AAV8>AAV1>AAV6>AAV2 Hippocampus AAV9...will depend upon which section you’re looking at (striatum, hippocampus and cortex) within the mouse brain...serotonin in movement. Follow her on twitter @katrinaboards.   References 1. Russell, David W. "AAV vectors...

...This post was contributed by Alessia Armezzani, scientific communication manager at genOway. A few decades...Hegemann, 1991). These proteins control the flow of electric charges across cell membranes and maintain membrane...neurons have in common? Neuronal stimulations are triggered by the movement of ions across the axon's membrane... is reached and the neuron fires, sending the electrical signal down the axon (Lodish et al., 2000). Most...light activates channelrhodopsins that, in turn, trigger neural excitation, whereas yellow light activates...stimulations, they simultaneously recorded the electrical impulses of discrete subpopulations of this brain...Through a partnership with genOway, we are able to distribute materials containing FLEx technology. Read the...

...intensity, excitation and emission spectra. The lab of Dimitri Deheyn recently identified a family of GFP proteins... that they can be imported to the mitochondrial matrix. Gottlieb’s lab has used an inducible MitoTimer...into Golden Gate entry vectors via traditional restriction enzyme cloning, TA cloning, or annealing of oligonucleotides...team at the Institute of Science and Technology Austria, decided to take a novel approach to control for...activity levels and limited sensitivity under the stringently low expression conditions necessary to avoid ...chemogenetic technologies After several years of distributing his Designer Receptors Exclusively Activated...cloning strategy, MoClo, which uses the Type IIS restriction enzymes BsaI and BpiI/BbsI to efficiently assemble...

...strengthened during memory formation and memory retrieval. In neuronal tracing experiments, brain tissue...Traditional tracing compounds either produce a colorimetric product or are fluorescent. While dyes usually...pressure injection or iontophoresis (applying small electric currents via inserted electrodes) may be applied...both antero- and retrograde labelling, synaptic restriction as well as controlled monosynaptic and polysynaptic...polysynaptic spreading. AAV vectors cover some of these attributes and their use has allowed for the visualization...recombinant AAV serotypes 1, 2, 5 and 8 in the rat nigrostriatal system. J Neurochem 109, 838–845. PubMed PMID...PMID: 19727139. Ugolini, G., Kuypers, H.G., and Strick, P.L. (1989). Transneuronal transfer of herpes ...

...use restriction digest to screen your clones, but there are many other ways to choose restriction enzymes...blue-white screen. Image from Stefan Walkowski.   Restriction digest Another way to distinguish between colonies...inserts from those that do not is by using a restriction digest. The key is to be strategic about your...route, check out our protocol video on using restriction digests to analyze plasmids, which can help you... you visualize this process, or visit our restriction analysis blog post. Colony PCR Colony PCR can...

...construct for C. elegans gene knockdown Article contributed by Alyssa Cecchetelli  Listen to the C. elegans... labeling with TurboID and miniTurbo Article contributed by Eric Perkins  Listen to the TurboID and miniTurbo...compartments is via proteomics--the study of the distribution and interactions of proteins within these structures...small-molecule substrate to map the spatiotemporal distribution and interaction networks of specific proteins...Changing the way we detect viral outbreaks Article contributed by Izzy Mueller  Listen to the viral detection... reveals novel peptide ligand motifs Article contributed by Michael Lemieux  Listen to the SH3 domain ...

...identify. Improved Restriction Enzyme Handling - SnapGene’s software positions restriction enzyme annotations...paste functionality. Restriction Enzymes Here you'll find a table of restriction enzymes that cut the...previous mapping software and now includes Type IIS restriction enzymes that are commonly used for Golden Gate...clicking on column heads. Clicking on a particular restriction enzyme here also highlights it in the “Map” and...

...from AAV Purification by Iodixanol Gradient Ultracentrifugation First, you’ll want to identify the reader...for AAV Purification by Iodixanol Gradient Ultracentrifugation. The reader may be, for example, a graduate...between a gradient tube before and after ultracentrifugation so that a scientist can extract virus from...interfaces between layers are blurred after centrifugation and the syringe needle is inserted bevel-up...profile photo of an iodixanol gradient after ultracentrifugation. The interfaces between layers are muddled...illustrates a gradient tube before and after ultracentrifugation. The viral supernatant migrates through increasingly...

...make efforts to keep it updated with new entries and update entries as research progresses, but you are invited...Additionally, the Phylogeny tool allows users to browse entries by the current phylogenetic nomenclature and discover... database, and all researchers are invited to contribute to it! The CasPEDIA Consortium will, of course... invited to reach out to suggest new entries or even new features to the tool. Resources and References...References References Adler, B., Trinidad, M., Bellieny-Rabelo, D., et al. (2023). CasPEDIA Database: A Functional...

...coupled with heterotrimeric G proteins on the intracellular side of the cell membrane. Heterotrimeric G proteins...in signal transduction pathways and consist of a trimer of three subunits: alpha (Gα), beta (Gβ), and gamma...the GPCR allows for binding of a new inactive heterotrimeric G protein complex and subsequently another ...elucidate the role of 14 of the 16 non-visual heterotrimeric G proteins that can be activated downstream... alpha, 2 beta, and 4 gamma subunits of the heterotrimeric G protein complex that can be used to detect...

...March 2015, Addgene reached the 500,000 plasmids distributed milestone! We were thrilled to see the impact...one plasmid deposited at Addgene that has been distributed more than 100 times.   “I really like this award... labs worldwide use our plasmids. I hope they contribute to great discoveries!” — Julien Béthune, Professor...Flame Award on Bluesky. In 2016, we also began distributing ready-to-use viral vectors from some of our ...supportive educational resources. By 2017, we had distributed 1,000 ready-to-use viral vector preps. Today,...citations of individual items in our repository, a tribute to how many discoveries have been made because ...

...your cloning strategy: Restriction enzyme digestion Be mindful of the restriction enzymes you chose. Make...This post was contributed by Oskar Laur, head of the custom cloning core at Emory University, and Paolo...which technique you are going to adopt (i.e. restriction cloning, Gibson Assembly, Golden Gate etc.), ... fragments of the size you expect, check the restriction pattern of the enzymes you chose, and verify ...hopefully have some new strategies to tackle that tricky cloning project. Good luck with your experiment...