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  4. Plasmids 101: NGS Quality Control for Pooled Libraries

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    Blog Post
    ...then scraped off the plates and pelleted for DNA extraction. Prepare library product for next-generation ...next-generation sequencing Once the DNA has been extracted, the representation of the library should be confirmed using...

  7. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...of that, perhaps you were involved in some extracurricular activities – did you volunteer to do science..., University of British Columbia. Originally on track for a research career, she transitioned into a communications...

  8. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...you’re within a year from the end of your PhD or contract, you should be starting to send out polite emails...can. You know you are in the zone when you lose track of time because you are engrossed in a task. Things...

  10. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...tasks. EvolvR could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks... of PolI3m. This tag would allow researchers to trace the progeny of a single starting cell. Additionally...

  11. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...Washington DC (#SfN17). This massive conference attracted over 30,000 attendees (scientists and nonscientists...people ready to discuss their VR helmets, gait tracking technologies, and brain monitors. Walking around...

  12. A Deep Dive into BioBuilder

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    Blog Post
    ...Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription and translation in vitro...and test hypotheses. Students then use cellular extracts as a means to rapidly prototype DNA circuits and...

  14. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...CRISPR Nucleases   Cas9 Cpf1 Components crRNAtracrRNACas9 crRNACpf1 Endonuclease size spCas9:...gRNA length gRNA: ~100 nt crNA: ~42 nt Requires tracrRNA? Yes No dsDNA cleavage Blunt end 5' overhang...

  15. Addgene’s Viral Service - Why Virus? Why Now?

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    Blog Post
    ... and careful Addgenies, but also a lot of good tracking. Our Software and IT Teams made major upgrades...inventory management systems to enable efficient, tracked production and distribution of viral samples. The...

  16. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...components. Transfer cPPT Central polypurine tract; recognition site for proviral DNA synthesis. Increases...transcription started by 5' LTR by the addition of a poly A tract just after the R sequence. This post was written...

  20. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...through our deposit process, and then we carefully track samples through our lab using barcoded tubes and...Your Plasmid Learn How Addgene Uses Barcodes to Track Samples Resources on the Addgene Website View ...
Showing: 221 - 240 of 505 results