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We narrowed to 762 results for: UTY

Showing: 221 - 240 of 762 results
  1. Summer SciComm Series: Master of SciComm

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    Blog Post
    ...communication graduates are often well-equipped. Many institutions are sincerely interested in connecting with ...of how to appeal to a given audience and how to utilize a wide array of media in doing so—can prove invaluable...risk perception, scientific uncertainty and precautionary regulation was all necessary to inform the experimental...unsettled science. Understanding just how the public utilizes insights gleaned from scientific research is, ...
  2. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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    Blog Post
    ...deftly coordinated by the eukaryotic cell for the execution of many complex cellular behaviors, such as differentiation...engineering realm of S. cerevisiae and E. coli. The utility of simultaneous and multidirectional multiplex ...off switch The multiple scRNAs all individually utilize the same dCas9 protein. By using an inducible promoter...Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt     References...
  3. FPbase: A new community-editable fluorescent protein database

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    Blog Post
    ...a single person. With thousands of scientists routinely using and thinking about FPs, it would be great... designed with the goal of encouraging user contributions (using simple web forms) while also maintaining...and we very much appreciate even those small contributions). Of course, no community-editable database ... include incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum...
  4. Plasmids 101: NGS Quality Control for Pooled Libraries

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    Blog Post
    ...versatile offerings! We recently re-amplified our distribution stock of the Brunello Human gRNA library, and...processes we use to ensure high-quality library distribution. You can also use these tips as a starting point... scaled to 1, to create a “cumulative wealth distribution” curve, or Lorenz curve. By calculating the ...gain a sense of the uniformity of the library distribution, with an AUC of 0.5 representing perfect uniformity...
  5. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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    Blog Post
    ...did To measure the pH of our samples, we used a solution of cell culture medium, which is buffered and ...room temperature and used the color of the liquid solution and pH paper to measure the pH. We compared the...note that the change in pH only occurred when the solution thawed, as the CO2 was confined to the tube headspace...et al., 2013). However, the pH changes in our solutions are transient and return to normal once the tube...
  6. What Good Citizenship Can Do for Reproducibility in Science

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    Blog Post
    ...(accession numbers, reagent details, author contributions, etc.) before their paper can be published. ...do. All of that helps, but it’s only a partial solution.  How do we get where we want to be? The part ... in science needs both a job and funding, so institutions and funders, to a large extent, hold the keys...across life and physical science, and editorial contribution to the strategic development of Cell Press. ...
  7. The MTA at Addgene

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    Blog Post
    ...commonly used ones for transfers between academic institutions.  What is the UBMTA, you ask? Well, organizations... agreement. The One-Time Plasmid Deposit and Distribution Agreement establishes the terms under which ... researchers additionally have the option of distributing under Addgene’s Industry MTA (IMTA), which allows...depositing institute that decides the terms of distribution for their plasmids. MTAs at Addgene Since Addgene...
  8. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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    Blog Post
    ...treatment options, as well as non-pharmacological solutions to modify the course of this condition are urgently...glowing bacteria and a fish robot to detect water pollution Dr. Julio Ernesto Valdivia Silva, Universidad ...related to space medicine. Now he aims on tracking pollution in rivers by employing synthetic biology resources...Biology Resources Learn about Global Plasmid Distribution ...
  9. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post
    ... also created. The depositors demonstrated the utility of light-mediated suppression of synaptic transmission...alternative such as a radioactive tracer. As a solution, Mikhail Shapiro’s lab has developed four acoustic.... At a very low volumetric density and with a resolution of less than 100 µm, the ARG reporters could ...group at the Harvard Medical School developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes...
  10. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

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    Blog Post
    ...developed in terms of the collection, storage, and distribution of the plasmids and their ability to identify...plasmids so scientists aren’t bogged down with distributing them and spending more time on research. What...plan manage risk. My job and my team’s job is to routinely assess the efficiency and effectiveness of the...auditor (CISA), and certified health insurance executive (CHIE). He is a member of both the Internal Institute...
  11. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post
    ...kinase activity can provide greater spatiotemporal resolution than pharmacological or genetic approaches, but...for creating optically controlled kinases. The Solution: In Zhou et al, the Lin lab present a generic ...domains on either side of the active site of a constitutively active MEK1. This photoswitchable MEK1 is called...Raf-MEK-ERK signaling pathway to demonstrate the utility of  photoswitchable kinases in vivo with C. elegans...
  12. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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    Blog Post
    ...concentration of the protein solution. During the experiment, the solution is diluted, complicating the...determine the capacity of homodimerization in aqueous solution. In cyto tests for aggregation To what extent ...the data presented in papers should be used with caution, since the difference between studies is substantial...
  13. Celebrating 15 Years of Scientific Sharing

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    Blog Post
    ...worry about and that is something to recognize! Distributing plasmids, viral service, and more For the ...about succeeding at our core mission. Plasmid distribution is amazingly still growing at >9% every year...since 2005. We’re meeting the challenges of DNA distribution to ensure scientists everywhere can access plasmids...Addgene. There’s so much potential for service and contribution! Leadership On an organizational level, ...
  14. Transferable Skills Guide: Problem Solving

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    Blog Post
    ...problem/task/situation, come up with potential solutions, and decide on a course of action that effectively...by paying attention to how your mentors reach solutions and ask them to explain their logic. Or you can... probably did not just automatically come to a solution. Consider the thoughts that went through your ... challenge yourself to think of more creative solutions. Ultimately, problem solving is less about technical...
  15. What's New in CRISPR - November 2021

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    Blog Post
    ...labs have recently published work that provides a solution to this problem. A miniature CRISPR-Cas system...describes an effective miniature Cas system that utilizes a Cas12f from a different species, Acidibacillus...mismatch repair (MMR) interfered with the desired resolution of prime editing intermediates. Building upon...systems improve gene editing performance at therapeutically relevant gene targets and expand the application...
  16. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post
    ...membrane. The haemagglutinin (HA) tag is a small 9 amino acid epitope of the haemagglutinin of the influenza...immunohistochemistry methods, these proteins are routinely fused to ‘tag’ or ‘label’ proteins to facilitate...nanogold particles, that allow a high-spatial resolution of the protein localization. Surprisingly our...
  17. Viral Vectors 101: Virus Safety

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    Blog Post
    ...it. This will help you understand what safety precautions to take, and if something does happen, ensure... retrovirus) and gamma retrovirus require more caution as they are integrating viruses – they physically... decontaminate in the waste itself, not in the solution that should be added. After decontamination, ... most common forms of lab accidents, so extra precaution should be used to reduce the risk of exposing...
  18. A Look at Addgene's QC Process

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    Blog Post
    ...to both people and the tools we use. Scientists utilize an array of tools that rely on DNA amplification...sequence discrepancies can be caught prior to distribution. Catching these errors prevents future experimental...can pass through QC and be made available for distribution. In these cases, the QC team leaves a note on...written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly...
  19. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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    Blog Post
    ...appreciate that you took the time to think of solutions, and will be more likely to view your proposals...viewpoint and brainstorm about mutually beneficial solutions Your supervisor might not agree with all of your...problem from their viewpoint, and brainstorm about solutions that will meet both of your needs. 6) Put important..., and discuss options for mutually beneficial solutions. If you package your challenges with your supervisor...
Showing: 221 - 240 of 762 results