...treatment options, as well as non-pharmacological solutions to modify the course of this condition are urgently...glowing bacteria and a fish robot to detect water pollution Dr. Julio Ernesto Valdivia Silva, Universidad ...related to space medicine. Now he aims on tracking pollution in rivers by employing synthetic biology resources...Biology Resources Learn about Global Plasmid Distribution ...

...commonly used ones for transfers between academic institutions.  What is the UBMTA, you ask? Well, organizations... agreement. The One-Time Plasmid Deposit and Distribution Agreement establishes the terms under which ... researchers additionally have the option of distributing under Addgene’s Industry MTA (IMTA), which allows...depositing institute that decides the terms of distribution for their plasmids. MTAs at Addgene Since Addgene...

...(accession numbers, reagent details, author contributions, etc.) before their paper can be published. ...do. All of that helps, but it’s only a partial solution.  How do we get where we want to be? The part ... in science needs both a job and funding, so institutions and funders, to a large extent, hold the keys...across life and physical science, and editorial contribution to the strategic development of Cell Press. ...

... also created. The depositors demonstrated the utility of light-mediated suppression of synaptic transmission...alternative such as a radioactive tracer. As a solution, Mikhail Shapiro’s lab has developed four acoustic.... At a very low volumetric density and with a resolution of less than 100 µm, the ARG reporters could ...group at the Harvard Medical School developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes...

...developed in terms of the collection, storage, and distribution of the plasmids and their ability to identify...plasmids so scientists aren’t bogged down with distributing them and spending more time on research. What...plan manage risk. My job and my team’s job is to routinely assess the efficiency and effectiveness of the...auditor (CISA), and certified health insurance executive (CHIE). He is a member of both the Internal Institute...

...kinase activity can provide greater spatiotemporal resolution than pharmacological or genetic approaches, but...for creating optically controlled kinases. The Solution: In Zhou et al, the Lin lab present a generic ...domains on either side of the active site of a constitutively active MEK1. This photoswitchable MEK1 is called...Raf-MEK-ERK signaling pathway to demonstrate the utility of  photoswitchable kinases in vivo with C. elegans...

...concentration of the protein solution. During the experiment, the solution is diluted, complicating the...determine the capacity of homodimerization in aqueous solution. In cyto tests for aggregation To what extent ...the data presented in papers should be used with caution, since the difference between studies is substantial...

... Nobel Prize in Chemistry for his contributions to super-resolution light microscopy, comes from his recent...article “SRpHi ratiometric pH biosensors for super-resolution microscopy.” Endosomes are historically difficult... endosomes, the Hell lab designed their super-resolution pH indicators (SRpHi) biosensors to meet all ...

...worry about and that is something to recognize! Distributing plasmids, viral service, and more For the ...about succeeding at our core mission. Plasmid distribution is amazingly still growing at >9% every year...since 2005. We’re meeting the challenges of DNA distribution to ensure scientists everywhere can access plasmids...Addgene. There’s so much potential for service and contribution! Leadership On an organizational level, ...

...problem/task/situation, come up with potential solutions, and decide on a course of action that effectively...by paying attention to how your mentors reach solutions and ask them to explain their logic. Or you can... probably did not just automatically come to a solution. Consider the thoughts that went through your ... challenge yourself to think of more creative solutions. Ultimately, problem solving is less about technical...

...labs have recently published work that provides a solution to this problem. A miniature CRISPR-Cas system...describes an effective miniature Cas system that utilizes a Cas12f from a different species, Acidibacillus...mismatch repair (MMR) interfered with the desired resolution of prime editing intermediates. Building upon...systems improve gene editing performance at therapeutically relevant gene targets and expand the application...

...membrane. The haemagglutinin (HA) tag is a small 9 amino acid epitope of the haemagglutinin of the influenza...immunohistochemistry methods, these proteins are routinely fused to ‘tag’ or ‘label’ proteins to facilitate...nanogold particles, that allow a high-spatial resolution of the protein localization. Surprisingly our...

...it. This will help you understand what safety precautions to take, and if something does happen, ensure... retrovirus) and gamma retrovirus require more caution as they are integrating viruses – they physically... decontaminate in the waste itself, not in the solution that should be added. After decontamination, ... most common forms of lab accidents, so extra precaution should be used to reduce the risk of exposing...

...to both people and the tools we use. Scientists utilize an array of tools that rely on DNA amplification...sequence discrepancies can be caught prior to distribution. Catching these errors prevents future experimental...can pass through QC and be made available for distribution. In these cases, the QC team leaves a note on...written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly...

...appreciate that you took the time to think of solutions, and will be more likely to view your proposals...viewpoint and brainstorm about mutually beneficial solutions Your supervisor might not agree with all of your...problem from their viewpoint, and brainstorm about solutions that will meet both of your needs. 6) Put important..., and discuss options for mutually beneficial solutions. If you package your challenges with your supervisor...

... the pharmaceutical industry for years. They have proven very difficult to target therapeutically due ... high-affinity binding targets for potential therapeutic agents. Nevertheless, a glimmer of light remains... important pathways. We invite feedback and contributions from all members of the research community as...

...potential gene drives should be conducted with extreme caution. Why does making transgenic organisms with Cas9...would find our work and consequently use these precautions in their own experiments2,3. While that didn'...blogger! An inventor of technologies that harness evolution, Kevin Esvelt studies ways of using molecular ...transparently.  For more information, visit www.sculptingevolution.org. References: Burt, A. Site-specific...

...engineered DNA systems capable of storing data and computing inside living cells. He's also a co-founder of...digital genetic switches that enable cellular computing. These logic gates built from transcriptors are... this way: "Addgene is one of the leaders in distributing plasmids amongst the academic community. On ...anticipate plugging into the probiotic and cellular therapeutic space. If you were to look forward to the future...

...there are benefits to optimized libraries that utilize only a few sgRNAs per gene. These libraries provide...., 2016, Sanson et al., 2018). These libraries utilize optimized sgRNAs that were designed based on the...while bypassing these issues. CRISPRi libraries utilize an engineered Cas9, which contains multiple point...transcription factors. The Root and Doench labs utilized tracrRNAs with two PP7 stem loops to recruit additional...

... chemistry and evolution to illuminate biology and enable next-generation therapeutics. Prime editing,...noise to each of the terms which make up our distribution, resulting in the following hierarchical model...University. He is interested in using selection, evolution, and machine learning to solve difficult problems...