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We narrowed to 577 results for: Vit

Showing: 221 - 240 of 577 results

  1. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...from the 3’ end, while its native endonuclease activity degrades the displaced strand.   Figure... the nick site, which was expected since the processivity window of PolI is 15-20 bp. EvolvR’s on-target...bacteriophage T7 DNA polymerase increased the processivity of PolI3M, and thereby increasing the editing...

  2. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...upon binding of CNO thus showing no constitutive activity. There are an array of DREADDs that activate different...neuroglia, and have different effects on neuron activity (firing or inhibition). AAV preps from DREADD ...chemogenetics and a concise table of DREADDS and their activity in neurons on Addgene’s chemogenetics guide.   ...

  3. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...the insert. Gateway® technology (developed by InvitrogenTM) is essentially a more modern version of this...CcdB-resistant strain is ccdB Survival™ from InvitrogenTM. Using either DB3.1 or ccdB SurvivalTM, one ...a variety of useful resources and protocols! We invite feedback from all members of the research community...

  4. RNA Extraction Without A Kit

    Type
    Blog Post
    ...in cells, and without something to block RNase activity upon cell lysis, RNA is degraded. To effectively...-based solutions that work by inhibiting RNase activity within cells or tissues- they don’t actually chemically...A (1974) The effect of ammonium sulfate on the activity of ribonuclease A. Biochimica et Biophysica Acta...

  5. Summer SciComm: Preprints

    Type
    Blog Post
    ...ability to post a preprint as evidence of their productivity that can be shared with hiring and review committees...encourage and consider preprints as demonstration of productivity.  Part of an Open Scientific Communication Ecosystem...details that you couldn’t fit on your poster, or invite attendees to read and comment online! Want the ...

  6. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ... you to share and build support for your ideas, invite others into decision making processes, and even...usually) presenting either to share knowledge or to invite critique onto an ongoing project. So whenever you...Conflict Resolution Transferable Skills Guide: Creativity...

  7. Working with Nuclear Receptors

    Type
    Blog Post
    ...sensors for a diverse set of fat-soluble hormones, vitamins, and dietary lipids. Included in this family are...progesterone, androgens, and estrogens), fat-soluble vitamins A and D, thyroid hormone, fatty acids, oxysterols...transfection platform for assaying transcriptional activity, for example, was a key development in the functional...

  8. Engaging with science and society at pgEd

    Type
    Blog Post
    ...personal identity. We do this through a variety of activities, including creating educational materials and...addition to events that we put on, we are often invited to present our materials or speak about our experiences...our science communication posts Extracurricular activities for a strong science career path Resources on...

  10. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...interact.  When this interaction occurs, BirA* activity is restored, and nearby proteins are labeled with...BirA* enzyme. When this interaction occurs, BirA* activity is restored, and nearby proteins are labeled with...proteins Y and Z part ways, BirA* biotinylation activity ceases. After biotin tagging of proteins in both...

  12. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...sequence (Green et al, 2014). The second was an in vitro paper-based platform that allowed gene circuits ...more stringent, and it avoided any days of lost productivity waiting for follow-up DNA oligos to arrive. ...parallel. More broadly, we drew on the combined creativity and expertise of the group and yet never hesitated...

  13. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...-base sequence. Double mismatches ablated Cpf1 activity, except when they were present in the 3’ end of... as Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the 3’ end of the target...found that both orthologs show lower off-target activity than that previously observed with SpCas9. Kleinstiver...

  14. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ... (500 to 580 nm) over the course of 18 hours in vitro (see figure 1B in Terskikh et al.) and even displays...showed that their versatile timer could be used in vitro, in mammalian cells, in C. elegans, and in Xenopus...changes in oxygen, temperature, and pH, but timer activity can be calibrated to these different conditions...

  15. Common Injection Routes in Mice

    Type
    Blog Post
    ...Intraperitoneal (IP) injections are given in the peritoneal cavity, or the space surrounding organs in the abdomen...organs. Restraint: scruffing Location: peritoneal cavity Gauge: 25–27 Volume: < 2–3 ml Skill required: intermediate...tail vein) Intraperitoneal Scruffing Peritoneal cavity 25–27 < 2–3 mL Intermediate Intramuscular Mechanical...

  16. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...cell-associated (Vandenberghe et al., 2010). For certain in vitro and in vivo applications, you can consider using..., E., Hernandez, A., Boye, S. L., Boye, S. E., Levites, Y., … Golde, T. E. (2020). Utilizing minimally...assay for adeno-associated virus vectors with sensitivity sufficient to detect single infectious events...

  17. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...Plasmids containing gRNA and Cas9 are used in in vitro transcription reactions to generate mature Cas9 ...ribonucleoprotein complexes Purified Cas9 protein and in vitro transcribed gRNA are combined to form a Cas9-gRNA...nor are these methods limited exclusively to in vitro cell culture. The user should review the current...

  18. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...teamwork, communication, writing, management, and creativity; I have developed these skills through school...not just “do the job”. When they ask about your creativity, your potential manager wants to hear a specific...resolution Organization Leadership Problem Solving Creativity Data analysis Listening Negotiation Career Planning...

  19. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...analyzed, the Fujii lab also developed another "in vitro" form. In this form, the CRISPR complex, consisting...biotinylated gRNA, were successfully used in this "in vitro" form. Any tag system can be used to isolate genomic... isolation of DNA fragments and chromatin by in vitro enCh IP technology using recombinant CRISPR ribonucleoproteins...

  20. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...to small-molecules: red-shifted spectra, ion sensitivity, photoactivation, etc. These impressive advances...of the genetically encoded tag, the speed and selectivity of the fluorophore attachment, the brightness...William Perkin. His discovery set off a flurry of activity and the majority of the classic small molecule...
Showing: 221 - 240 of 577 results