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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 315 results for: bon;

Showing: 221 - 240 of 315 results

  1. Tetracycline Inducible Expression

    Type
    Collection
    ...tet-inducible vectors. Tetracycline-inducible Backbones Find backbones or constructs to use for cloning to control...Browse the tables below to find popular empty backbones, expression vectors for transactivators, and vectors...inducible RNAi with miR-E (miR-30 variant)-based backbone rtTA-Advanced TRE3G Johannes Zuber 60495 pSBtet-GP...Tight TRE David Sabatini 19407 pTREtight2 Empty backbone for Tet-controlled expression (On or Off) None...transactivator under the c-Fos promoter Tet-On 3G rtTA Bong-Kiun Kaang 20342 FUW-M2rtTA Lentiviral Tet-On vector...

  2. Fluorescent Proteins: FRET

    Type
    Collection
    ...properties. Where available, we have listed cloning backbones and control plasmids. Donor Acceptor λ Dex QY ...Amber (mVenus Y67C) FRET standards and cloning backbones Koushik et al. (2006) Cerulean, Venus, and VenusY67C...FRET Positive and negative controls and cloning backbones for mTurquoise2-mVenus(L68V) Goedhart et al. (...93% Positive and negative controls and cloning backbones for various FRET pairs with mTurquoise2 Mastop...mTurquoise2 Positive and negative controls and cloning backbones for red, yellow, and turquoise FRET pairs Gadella...

  3. CRISPR Guide

    Type
    Collection
    ...been created in AAV backbones for in vivo experiments and in a retroviral backbone for delivery to cells... gRNA and Cas9 protein, which will form a ribonucleoprotein complex through interactions between the gRNA...disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading and discrimination...endonuclease. Nature , 513 (7519), 569–573. PMID: 25079318 Bondy-Denomy, J., Pawluk, A., Maxwell, K. L., & Davidson...system. Nature , 493 (7432), 429–432. PMID: 23242138 Bondy-Denomy, J., Garcia, B., Strum, S., Du, M., Rollins... Maddalo, D., Manchado, E., Concepcion, C. P., Bonetti, C., Vidigal, J. A., Han, Y., Ogrodowski, P., Crippa...Marino, N. D., Pinilla-Redondo, R., Csörgő, B., & Bondy-Denomy, J. (2020). Anti-CRISPR protein applications...

  4. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...what vector to use, you can check out our Empty Backbone Reference . Background Subcloning by restriction...and purified your insert and recipient plasmid backbone bands away from the gel via your favorite gel ...have of uncut or self-ligating recipient plasmid backbone. Transformation Transform your ligation reaction...

  12. March for Science

    Type
    Blog Post
    ...Scientists' Views PolitiFact: EPA head Scott Pruitt says carbon dioxide is not 'primary contributor' to global ...

  16. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...for nearly any piece of DNA to be placed into a backbone vector of choice with minimal limitations. Background...MCS)), but do not cut elsewhere on the plasmid. Bonus: It is helpful to choose restriction enzymes that...have of uncut or self-ligating recipient plasmid backbone. Transformation Proceed with the transformation...

  17. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab....Davidson Fluorescent Protein Collection Empty Backbones Blue Cyan Green mEmerald Yellow Orange Red Photoactivatable...Davidson lab has contributed the corresponding empty backbone with different fluorescent tags for you to create...tutorials, microscopy protocols, and more. Empty Backbones Plasmid Description Industry Return to top Blue...
Showing: 221 - 240 of 315 results