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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 335 results for: c rel

Showing: 221 - 240 of 335 results

  1. Let There Be LITE Plasmids

    Type
    Blog Post
    ...groups making use of the new optogenetic tools in C. elegans and in Plasmodium. For those who do want ...those optogenetic controls, gene expression levels reliably increased in vitro by a factor of 10 to 20. A ...modifications in the genome,” Zhang said in MIT’s press release. In an accompanying Nature News & Views Andreas...

  2. Bacterial Expression Systems

    Type
    Collection
    ...peptides include: Epitope tags: 6xHis, Flag, Strep II, c-Myc, HA, V5, GST Solubility tags: MBP, SUMO, TrxA,...reconstructed) BiFC Lynne Regan 168257 168472 pMRBad-C-wtBlc pET11a-N-DiB2 DiB2 (reconstructed) BiFC Jens...coli Cynthia Collins 112197 pANY3 Temperature (42 °C) Escherichia coli Yingfeng An Other Addgene Controlled...interactions using fluorescence microscopy. FRET relies on the principle that when a donor fluorophore ...for a selection of mammalian and bacterial FRET-related vectors and standards. The Hoschschild Bacterial... that vary in strength and that can be used to reliably drive expression of a gene of interest in E. coli...

  3. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...similar nuclease folds found in other proteins. The C-terminal domain (CTD) of the protein also belongs ...https://doi.org/10.1038/s41586-022-04470-1 Cofsky, J. C., Soczek, K. M., Knott, G. J., Nogales, E., & Doudna...Sternberg, S. H., Redding, S., Jinek, M., Greene, E. C., & Doudna, J. A. (2014). DNA interrogation by the...the enzyme needs cellular recycling factors to release its handiwork (Sternberg et al., 2014). New discoveries...

  4. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...; CDSns, coding sequence with no stop codon; TAG, C-terminal tag; TER, terminator. Created with BioRender.com... is very important that these stay frozen at -80 °C, since multiple rounds of freezing and thawing will...in what position do you want your gene to be in relation to the rest of the genes? And do you want it to...

  5. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...solubility (Monnier et al., 2013). The linker keeps the C-terminus of one variable domain and the N-terminus...10.3390/antib2020193  Khantasup K, Chantima W, Sangma C, Poomputsa K, Dharakul T (2015) Design and Generation...to avoid animal use by using semi-synthetic or entirely synthetic VL and VH sequences. Phage display also...scFvs are also used for eye diseases, such as age-related macular degeneration (AMD) since direction injection...of the CAR facilitates signal transduction and release of cytotoxic granules from the T cell. When a CAR... CAR has an scFv that binds cancer-related antigens, binding of the scFv part of the receptor can activate...

  6. How to Write a Scientific Review Article

    Type
    Blog Post
    ... all the proteins in the class that showed the relative sizes and the conserved domains. This information...perfect. Good luck! References 1. Haery, Leila, Ryan C. Thompson, and Thomas D. Gilmore. "Histone acetyltransferases... maybe you’re researching a topic for which no relevant or recent review exists. Once you pick a topic...format also has the benefit of exposing you to relevant researchers in the field. Finally, you can sound...from start to finish. Find the information that’s relevant to you, note it, and move on. At the beginning...

  7. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ... deliver a large amount of DNA (~8 kb) with a relatively low immune response, it should come as no surprise..., Andre K, Stein D, Blick G, Greenberg RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas P, June CH, Humeau... co-transfection of these plasmids, 293T cells released transgene-containing lentiviral particles into...

  9. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...reaction takes an hour or less to complete at 50 °C. After an hour or so, the sample is immediately ready...PMID: 25861928. 3. Jiang W, Zhao X, Gabrieli T, Lou C, Ebenstein Y, Zhu TF. Cas9-Assisted Targeting of CHromosome... traditional restriction enzyme cloning we once relied exclusively on. In this blog post, I will go over...isn’t ideal for synthetic biology standards, which rely up on the re-use of parts between experiments. In...

  10. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ... controls how many false positives are allowed relative to true positives. Both metrics can be used to...and plot your data in ascending numerical value relative to quantiles derived from a theoretical distribution...measure this effect, known as covariance. This is the “C” that differentiates these tests from ANOVA/MANOVAs.... Below are several core factors of statistics relevant for many downstream statistical tests.   Independent...significance exists somewhere amongst multiple groups is rarely enough information. A post hoc test, done after...yourself from family-wise error and just perform the relevant comparisons using Dunnett’s test, a test that ...

  11. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...post-translational modifications, quantify protein levels relative to standards, detect the cellular location of ...thickness of the band provides information about the relative abundance of the protein in the sample. More protein...4354 . https://doi.org/10.1073/pnas.76.9.4350 Yang P-C, Mahmood T (2012) Western blot: Technique, theory,...endogenously expressed protein at physiologically relevant levels. Some vendors will also test their antibody...known to express the protein at physiologically relevant levels (not overexpression systems) while negative...molecular biology protocols Browse other articles related to antibodies Resources on Addgene.org Find written...

  12. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...expressed GFP in exogenous organisms (E. coli, and later C. elegans) for the first time. After Chalfie’s work... in molecular detail. On the underside of the “umbrella” of A. victoria there is a ring that glows with...

  13. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...PubMed Central PMCID: PMC1559948. Brusca, Richard C., and Matthew R. Gilligan. "Tongue replacement in ...membrane. Vampirovibrio chlorellavorus will not grown in culture alone and relies on its algae host for ...ant’s body. The fruiting bodies then rupture and release spores onto the forest below for the next unsuspecting...stumble upon. Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria that attaches to the...adult worms develop in the digestive tract and release eggs in the bird feces, which get eaten by another... the predatory cyanobacterium Vampirovibrio chlorellavorus." PeerJ 3 (2015): e968. PubMed PMID: 26038723...

  14. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...Sato T, van Es JH, van den Born M, Kroon-Veenboer C, Barker N, Klein AM, van Rheenen J, Simons BD, Clevers...melanogaster. Hadjieconomou D, Rotkopf S, Alexandre C, Bell DM, Dickson BJ, Salecker I. Nature Methods. ...observed in a given area. In 2013, Dawen Cai, et al. released a refinement of the Brainbow technology, Brainbow...

  15. Synthetic Biology - Overview

    Type
    Collection
    ...Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard Endy...Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification, including ...and tagging vectors. Metabolism Browse plasmids related to metabolic pathways and auxiliary components....networks. Sensing and Signaling Browse plasmids related to intercellular signaling and environmental sensing...

  16. Institute for Protein Innovation

    Type
    Collection
    ...table below to find antibodies targeting V5, Protein C, DYKDDDDK, Rho, His, Biotin, EE, GCN4 and Strep. Please...presence and explore glypican function, providing a reliable and accessible toolset for scientists worldwide...

  17. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...expression vector. All His-tags are cloned at the C-terminus of MDH and, if indicated on the plasmid map...support educators interested in integrating MDH-related research into their teaching laboratories. They... plasmids, faculty can begin a sustainable MDH-related CURE focusing on protein expression, structure ...

  18. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...ask at least one question to clarify the situation; C = confirm that you have understood the speaker accurately...brings feedback back to the manager. Team members rarely rush to give honest feedback to the manager for...

  19. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ..., G. S. Parker, S. J. Qualls, B. M. Lewis, E. Ke, C. T. Dargitz, R. Wright, A. Khanna, F. H. Gage, and... Mikkers, J. Kool, L. van der Weyden, A. H. Lund, C. H. Wilson, R. Rance, J. Jonkers, M. van Lohuizen,... procedure is cumbersome and inefficient, as it relies on random integration efficiencies that may be ...off-target effects is likely due to the fact that PBx relies on the inverted terminal repeats (ITRs) found on...

  20. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...hemocytometer or other cell counter. Pro-Tip Prepare a relatively dilute cell solution (<10 6 cells/mL) before ...heat-inactivated FBS and 5 mL of 100X glutaGRO. Store at 4 °C. Polyclonal stable cell pool: see our protocol for...
Showing: 221 - 240 of 335 results