Plasmids 101: Protein Expression
Type
Blog Post
...acids. Cell-free expression systems are ideal for fast assembly of more than one protein in one reaction...
We narrowed to 273 results for: fas
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Transferable Skills Guide: Managing a Team
TypeBlog Post...exactly what and how to do it so shouldn't it be faster to do it yourself? Training certainly takes time... -
Academic vs. Industry Postdocs
TypeBlog Post... environment, have a better understanding of the fast, deadline-driven pace of biotech. In other words... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...NHEJ joins the ends of a DSB in an error-prone fashion, with insertions and deletions common. In contrast... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...Addgene Sequences’). These sequences are provided in FASTA format (example) and can be analyzed using Addgene... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post...for genome editing purposes because it's cheaper, faster, and easier than prior editing techniques. More... -
Which Fluorescent Protein Should I Use?
TypeBlog Post...The four main FTs are called Slow-FT, Medium-FT, Fast-FT, and mK-GO. Large Stokes Shift (LSS): Stokes ... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post...technologies, proposing that “Addgene, with its easy, fast, and cheap distribution of the newest CRISPR-Cas... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post...improved search engine to help make this task a faster, easier, and frustration-free experience. Along... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post...genome engineering technology is moving along much faster than it can be officially regulated. I did not ... -
Summer SciComm Series: Modes of Communication
TypeBlog Post...information you want to convey. Small tidbits and fast/fun facts are easy and engaging on social media,... -
AAVs in Retinal Gene Therapy
TypeBlog Post...called Retinitis Pigmentosa. I quickly became fascinated by this emerging technology and its potential... -
CRISPR Antimicrobials
TypeBlog Post...phages and antibiotics. Phage therapy fell out of fashion with the introduction of antibiotics, but the field... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...upstream of the PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post...short period of time and may need to do some fashionable projects. However, I hope that they also have... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post...safety when they leave home which happens very, very fast! I will never forget the time my eight-year-old ... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog Post...higher!) format. Liquid handling robots are not only faster and more accurate, but they also work with very... -
Hot Plasmids: Fall 2024
TypeBlog Post...greater photostability and also matured considerably faster, with expression levels comparable to GFP during... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post..., and jGCaMP8 appears to be growing in requests faster than jGCaMP7 (Figure 5B). Figure 5: ... -
Antibodies 101: The Four ELISAs and When to Use Them
TypeBlog Post...method Method Advantages Disadvantages Direct Fast and simple protocolReduced chance of cross-reactivity...