We narrowed to 326 results for: iNOS

1. Sharing is Caring: But How to Distribute Open Hardware?

   Type

   Blog Post

   ... available tools such as the Raspberry Pi, the Arduino, or 3D printers are key to enabling this work. ...

2. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   Blog Post

   ...phage recombination machinery is induced using L-arabinose. These cells can be made and frozen in large batches...

3. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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   Blog Post

   ...ChIP-Seq results. The FLAG epitope is quite small—20 amino acids—so we don’t think it interferes with the function...

4. Plasmids 101: Blue-white Screening

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   Blog Post

   ... enzyme. Providing DNA encoding this section of amino acids (called the α-peptide) to a lacZΔM15-mutant...

5. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   Blog Post

   ... CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

6. 3 Challenges in Plant Synthetic Biology

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   Blog Post

   ...Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in 2004, she accepted ...

7. Plasmids 101: Yeast Vectors

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   Blog Post

   ...L-lysine yes - Grow on plates containing alpha-aminoadipate in the absence of a nitrogen source. S. cerevisiae...

8. Protocol - pLKO.1 – TRC Cloning Vector

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   Protocol

   ...Sigma #A9539 D.2 Screening for Inserts Day 1: 1. Inoculate 5 colonies from each ligation into LB + 100 μg...

9. RANbodies: Reporter Nanobody Fusions

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   Blog Post

   ...that’s encoded on a plasmid. A nanobody is a ~150 amino acid, single domain antibody. To create their RANbodies...

10. Transferable Skills Guide: Cross-team Communication

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    Blog Post

    ...first weeks or months when I was learning the terminology of a new field. Annotate: When creating important...

11. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post

    ...cell 21.3 (2012): 309-322. PubMed PMID: 22439926. Hoshino, Ayuko, et al. "Tumour exosome integrins determine...

12. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post

    ...protein (mScarlet) with either a seven or eight amino acid linker between them. The Ace-mScarlet voltage...

13. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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    Blog Post

    ... plans. 2 of those raising - the University of Illinois and the University of Michigan - initially cancelled...

14. Antibodies 101: Affinity Reagents

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    Blog Post

    ...variety of targets, including proteins, peptides, amino acids, drugs, metal ions, and cells. They can be...

15. What's New in CRISPR - November 2021

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    Blog Post

    ...efficiency. The lab also optimized the architecture and amino acid sequence of the prime editor, yielding PEmax...

16. Developing Lab Management Software for Biology

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    Blog Post

    ...about how they would like these things tracked. Terminology and language are tricky Names for things will...

17. Addgene's Tips for Plasmid Quality Control

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    Blog Post

    ... sequence from a typical reaction, or about 250 amino acids worth, so make sure the feature you want to...

18. Plasmids 101: Golden Gate Cloning

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    Blog Post

    ... with an attB recombination scar encoding eight amino acids, but Golden Gate assembly can be designed ...

19. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post

    ...Thomas, D., & Surdin-Kerjan, Y. Metabolism of sulfur amino acids in Saccharomyces cerevisiae. Microbiol Mol...

20. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    Blog Post

    ... editing efficiencies of the chimeric and fully albino mice ranging from 11% to 100%, a level similar ...