Hot Plasmids: Fall 2024
Type
Blog Post
...fluorescent proteins will be a useful addition to the toolkit for visualizing cellular processes in C. elegans...
We narrowed to 240 results for: kit
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CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog Post...removed by sacB counterselection. The MUCICAT toolkit includes versions of plasmids with different induction... -
Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs
TypeBlog Post...15557121. Pubmed Central PMCID: PMC2172584. 11. Jokitalo, Eija, et al. "Golgi clusters and vesicles mediate... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...Bold Data Policy - by David Crotty (The Scholarly Kitchen) Read more Addgene blog posts about scientific... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post...ingredients reagents permeate the molecular biologist’s kitchen lab - and scientists using aptamers might know ... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post...pSer-incorporated proteins requires a molecular toolkit containing the OTS, an appropriate bacterial host... -
Hot Plasmids: Spring 2025
TypeBlog Post...One of the most powerful tools in a scientist's toolkit is the empty plasmid backbone: the DNA vector that... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...2006). Adeno-associated virus serotypes: Vector toolkit for human gene therapy. Molecular Therapy: The ... -
Antibodies 101: Validation
TypeBlog Post...Antibodies are a critical component of the researcher toolkit due to their ability to bind to molecular targets... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog Post...Producing Recombinant Antibodies An Integrin Antibody Toolkit from IPI Additional resources on Addgene.org ... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post... 19325077. PubMed Central PMCID: PMC2758053. 8. Okita, K., et al., Generation of mouse induced pluripotent... -
Using AAV for Neuronal Tracing
TypeBlog Post...K., Pignatelli, M., Rivest, A.J., Jung, H.-Y., Kitamura, T., Suh, J., Frank, D., Kajikawa, K., Mise, N... -
CRISPR Guide
TypeCollection...protein-interacting RNA aptamers, such as in the CRISPRainbow kit . Using multiple copies of the aptamer improves stability... -
Retrovirus Plasmids
TypeCollection...Zuber et al. 2011 for additional plasmids in this toolkit. Lowe 64865 pLncEXP CMV/MSV lncRNA expression plasmid... -
Open Enzyme Collection
TypeCollection...compatible with the E. coli Protein Expression Toolkit (Link opens in a new window) — all Open Enzyme ... -
CRISPR History and Development for Genome Engineering
TypeCollection...empowered researchers to further develop the CRISPR toolkit by posting lab protocols , providing tips from ... -
Tetracycline Inducible Expression
TypeCollection...article ( Meerbrey et al., 2011 ) for additional tookit plasmids for shRNA or gene expression. rtTA-Advanced... -
Adenovirus Guide
TypeGuide...Adenoviral Plasmids Available at Addgene AdenoBuilder Toolkit Viral Vectors 101 eBook Addgene's Biosafety Resources... -
Adeno-associated virus (AAV) Guide
TypeGuide...2006). Adeno-associated virus serotypes: vector toolkit for human gene therapy . Molecular Therapy, 14 ... -
Neurodegeneration Plasmid Collection
TypeCollection...Fawzi 127616 myc-KIF5A KIF5A Myc CMV ALS Josef Kittler 128510 pPHAGE-CR-PINK1-C-TAP PINK1 Flag, HA CMV...-eGFP-C1-VPS35 VPS35 GFP CMV Parkinson's Josef Kittler 163858 VPS35 shRNA1 VPS35 Parkinson's Lei Lu 163859...