...verify the identity and quality of the plasmids we make available and provide some advice on the steps you... information in a binder or digital notebook and make sure you note the freezer location of every plasmid...typical reaction, or about 250 amino acids worth, so make sure the feature you want to verify is within this...perform Sanger sequencing from glycerol stocks, so make sure to select the appropriate service and read ... a large number of sequencing reactions, you can make your life much easier by selecting the right primer...

...process. Again, thanks to our partners at seqWell we make use of a pipeline that takes the raw data from each... is useful, but not 100% complete, we will still make this data available as a partial sequence with the... load it on to our new MiSeq and set up the run making sure to note the appropriate barcode for each sample...described by Joung et al. 2017, which we hope to make available as part of our OpenBio Repository sometime...K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020...

...the development of a simple and scalable means of making targeted genetic edits. Cas9 is a DNA binding protein...Traditional Cas9 regulators In addition to its utility in making targeted modifications to DNA, Cas9 can be reprogrammed...modifying DNA and of modulating gene expression makes it an invaluable tool in the interrogation of gene...potent Cas9-based transcriptional regulator without making any functional changes to Cas9. For instance, when...been found to retain some ability to induce Cas9 to make edits (AC, WLC, and JQ, unpublished results). An...

...of this?” are all very valid questions, and often make an already complicated process even more difficult...program is the best fit for you and your goals. Making a List of Schools Creating a list of schools is... to highlight in the interview is another way to make the virtual environment more comfortable and help...presentation. It can help soothe your nerves and make sure you cover everything you need to during the...COVID-19, financial and logistical obstacles could make touring potential programs and universities stressful...

...sites, will result in similar sized bands, thus making this simple digest less informative. This is particularly...

...culture. Protocol Prepare liquid LB. For example, to make 400 mL of LB, weigh out the following into a 500...

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...improved learning outcomes and the opportunity to make new discoveries. Provost notes the importance of...excellent training in improvisation and the latter making him a more effective motivator (“be like Tony Robbins...thousands of undergraduates. The MCC develops and makes freely available technical and pedagogical resources...transform undergraduate education in the sciences by making a greater number of students active participants...Course-based undergraduate research experiences can make scientific research more inclusive. CBE Life Sciences...

...doi.org/10.1038/s41586-019-1323-z Peters JE, Makarova KS, Shmakov S, Koonin EV (2017) Recruitment of CRISPR-Cas...array. Workflow and technical considerations Making insertions in bacterial genomes with the V. cholera...Strecker J, Ladha A, Gardner Z, Schmid-Burgk JL, Makarova KS, Koonin EV, Zhang F (2019) RNA-guided DNA insertion...doi.org/10.1126/science.aax9181 Strecker J, Ladha A, Makarova KS, Koonin EV, Zhang F (2020) Response to Comment...

... high-speed internet. Before you begin any work, make sure you know if your audience will be able to access...if needed. And since we live in the digital age, make sure to research any software you may need: cost...communications you create. Wildlife biology and ecology can make for beautifully compelling photos and videos, while...things that cannot be seen with the naked eye or to make things more accessible by including, highlighting...Well-developed content creation platforms.  Algorithms can make it difficult to reach people who are not already...

...targeted by specific sgRNAs. Its RNA-guided nature makes CRISPRa much simpler to implement in genome-wide...the SPH activator paired with AAV sgRNA delivery make the SPH mouse an attractive tool to probe gene-function...screening strategies Although the tools described above make CRISPRa more accessible to neuroscientists, not ... in vivo applications, the tools described above make CRISPRa in the brain relatively easy and accessible...overexpressing genes within discrete cell populations makes Cre-dependent SPH mice an attractive alternative...

...clear plans to connect Genotype to Phenotype thus making progress on the NSF “G to P grand challenge.” Creating... are great for studying parenting strategies and make important ecological indicators since species live...possible to actually silence this gene with RNAi to make a polygamous species monogamous. Anolis sagrei (...CRISPR/Cas in lizards. The Menke Lab was able to make an albino lizard with targeted mutations. Now what...this to edited those brown marmorated stink bugs to make them pink. We’re excited to see new tools being ...

...regulate the Z pathway?" A well-defined outcome makes it easier for the search committee to understand... to be overly ambitious – a common mistake is to make each aim into a 10-year research program. It's a...communicate your story with only the most salient details. Make the most of your limited speaking time. Beyond ...vital task of engaging your audience. You need to make your audience care, to want to know the answer to...Creating a coherent unified story and model can help make your ideas easier to understand and more exciting...

...updated in several years) or they make significant changes that make it incompatible with our software...a problem, but it is these very differences that make our software so great. If we all came from similar...a bag of flour is not the only thing you need to make a cake. The flavor, shape, and texture of a cake...understanding the hows and whys of our users, we are able to make informed software design choices, resulting in software...

...structure and function of Cas13a with a focus on what makes this molecule unique, and the various applications...nuclease Cas13a was originally identified in 2015 (Shmakov et al., 2015). They were using Cas1, a gene commonly... in length and does not require a specific PFS, making it a very flexible cleavage system. LwaCas13a is...excited to see this group of enzymes continue to make a splash in the CRISPR field. We feel that the culture... Find plasmids from this paper at Addgene. 6. Shmakov, Sergey, et al. "Discovery and functional characterization...

...organism of choice. Unfortunately, codon preferences make it so you cannot choose among the possible codons...therefore stall at inappropriate locations or fail to make it through the entire transcript resulting in the...properly. You might then think, “Okay, I’ll just make sure the abundances of the codons I choose for the...surrounding codon choice, you should have some method to make sure the proteins you produce function properly....choice, you should perform a functional assay to make sure your protein isn’t forming inclusion bodies...

...exclusive access to data and enjoy reasonable time to make sense of it, with their decision to publish setting...the clock for others to dive in. The real cost of making data accessible A more reasonable concern with ...raised by researchers worried about the effort to make the underlying data easily available, organized ... the scientific community is thus not averse to making data available, but there remains concern about...reader as possible. Rather than adding to demands, making effective data sharing the norm should open up ...

...through some of the most important considerations to make when planning and performing an IF experiment. ...antibodies in a few different species and colors makes it easy to perform multiplexed IF. By using multiple...Some of the most important considerations are to make sure your secondary 1) will recognize the species...Database and CiteAb. The antibody you choose can make or break your experiment, so take advantage of all...your experience, positive or negative, will help make antibodies more effective and reliable in the future...

...Description (Link opens in a new window) Link to Video Making LB Agar Plates Create plates to culture bacteria...

...Two plasmids that can be used to make inexpensive 100 bp or 1 kb DNA molecular weight ladders were recently... plasmid prep protocol, it cost less than $10 to make 1000 lanes worth of the 100 bp or the 1 kb ladder... than commercial ladders. Are you interested in making your own DNA ladder? You can find the Tan Lab’s...Protocol Learn How to Analyze Your Restriction Digests Make Your Agarose Gel Other Resources Read about Gel...