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We narrowed to 449 results for: organization Addgene

Showing: 221 - 240 of 449 results

  1. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... structures give the cell its shape, organize cellular organelles, and are necessary for movement and ...of your protein of interest with known markers. Addgene has assembled a collection of fluorescent fusion... Plasmid Collections Fluorescent Proteins Organelle Markers Fluorescent Proteins: Subcellular Localization...fusion proteins that are targeted to various organelles or subcellular structures. You should fuse your protein...General Structures The cell contains a variety of organelles and subcellular structures each with their own...table below to find markers of general cellular organelles and structures. ID Plasmid Structure Targeting...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  3. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...plasmids you use to bring this into your target organism. We have designed a Golden Gate system based on...The complete GreenGate toolkit, now available at Addgene, also gives the option to stack several expression...motivations for making GreenGate available through Addgene,” Lohmann said. “We strongly believe the free sharing...  Kendall Morgan...

  4. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Along these lines, Darrell recently joined the Addgene Board of Directors where he hopes to help keep ...of this technique will someday allow us to make organs using iPS cells derived from patients. Chapter ...Chapter 12: Darrell's goals as a member of the Addgene board of directors - increasing open source sharing...Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids from the...

  5. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...protocols Sep-OTSλ plasmid - Addgene # 68292 C321.ΔA bacterial strain – Addgene #68306 pCRT7 NT TOPO E17TAG...E17TAG GFP plasmid - Addgene #68295 SupD plasmid – Addgene # 68307 For further technical reference and protocols...amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA synthetase, despite...flavors’ of Sep-OTS plasmids are available through Addgene, but we have worked most extensively worked with... recombinant phosphoproteins’ available on the Addgene website. Many thanks to guest blogger Natalie ... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156...Protoc. doi 10 (2008). Journal. Resources on the Addgene Blog Verify Your Constructs with Colony PCR Read...

  6. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...plasmids available from Addgene Capsid Published Tropism Model Organism Mouse Strain-Specific...administration, distributing widely throughout the organism to target large or diffuse biological structures...good option for your experiment? Look it up in Addgene’s Data Hub and the CLOVER repository of published...specific application and animal model.  Other model organisms Tropism and efficacy can vary depending on species...target cell type or tissue of interest, and model organism under study. *Many engineered AAVs for mice use...most commonly requested systemic capsid in the Addgene repository. Like its parent AAV-PHP-B, it can be... and shows decreased transduction in off-target organs (Goertsen, Flytzanis, et al., 2022). AAV-PHP.N ...

  7. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...should come as no surprise that those of us at Addgene believe in the power of sharing, and especially...ban gene patents in a case against Myriad, an organization called Free the Data launched a public database...And, last but not least (certainly not for us), Addgene launched this blog, A Better Way to Share Science...  Kendall Morgan...

  8. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0, 1.1, 2.1 plasmids...addition to the neuronal pThy1-Brainbow constructs, Addgene also has two Brainbow adeno-associated viral vectors...techniques are also being applied to study model organisms such as Drosophila and zebrafish. Brainbow in ...important insights into the complicated physical organization of the brain and other biological systems. Interested...

  9. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...our hot plasmids segment on the Addgene Podcast. Subscribe to the Addgene blog to get all the latest hot...transposon vectors tested in this work are available at Addgene. Kim, et al. Stem Cell Reports. 2015. PubMed PMID... using a viral approach Genetically modified organisms like transgenic mice are important tools for understanding...specific to interneurons in mice as well as other organisms, opening the possibility of using these tools ... a stable monomeric form as determined by the organized smooth endoplasmic reticulum (OSER) assay. A major...spines. The set of CyRFP constructs available at Addgene includes bacterial and mammalian mCyRFP1 or CyRFP1...lab has deposited all mScarlet constructs with Addgene. Bindels DS, et al. Nat. Methods. 2017. PubMed...

  10. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...executive director of Addgene, it is my privilege to uphold the values that shape Addgene’s mission and work...mirroring our own aspirations for Addgene. On behalf of every Addgenie, thank you for twenty years of incredible...thrilled to be sharing our plans for 2024.  In 2023, Addgene distributed 188,729 materials to 39,844 scientists... plasmids, deposited from 1,106 labs in 504 organizations from 37 different countries, into our repository... three–year project that touched every team at Addgene. As a resource, it will allow scientists around...Institute for Protein Innovation (IPI), in which Addgene will distribute recombinant antibodies (rAbs) developed...focus on expanding international access to the Addgene repository, partially through work with our distributors...

  11. What's New in CRISPR - September 2019

    Type
    Blog Post
    ... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...zebrafish genome, this system can be used in these organisms. Read the paper in bioRxiv Find the GeneWeld...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

  12. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...the observation of proteins in living cells and organisms. Both components of the chimera are encoded by...). However, these assays are qualitative. The Organized Smooth Endoplasmic Reticulum (OSER) assay (Costantini...PMCID: PMC4723041. Additional resources on the Addgene blog Read our fluorescent proteins blog posts ...Read our plasmids 101 blog series Resources on Addgene.org Browse the fluorescent protein collection Find...

  13. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...post was contributed by guest bloggers and IMP organizers Rosario F. Godino, Rocío López-Diego, & Zafira... Students also need more access to reputable, organized networks connecting international experts and ...Advancemnet of Higher Education website Resources at Addgene Read Other Career Blog Posts Read the Networking...

  14. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    ...Additional resources on Addgene.org Find molecular biology protocols here Visit the Addgene careers page ... of different phages or with phages targeting organisms closely related to the clinical sample. For treatment... Capsid and Tail. Additional resources on the Addgene blog Read our blog posts on open science Find ...

  15. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... resources on addgene.org  Addgene's CRISPR Guide Addgene's CRISPR 101 eBook  Addgene's CRISPR Cheat Sheet...distinguishing the foreign sequences and keeping the array organized. Target strand: The strand of foreign DNA that...looser PAM requirements, which are available from Addgene!   RNA crRNA: “CRISPR RNAs” are transcribed from...CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable guide sequence...Conclusion Ready for some more details? Check out the Addgene CRISPR guide or our CRISPR 101 ebook. We also ... Sheet  Resources on the Addgene blog A Needle in a Base-Stack: Cas9 Structural Biology...

  16. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...manipulation Resources on Addgene.org Browse all viral vectors available at Addgene Visit our viral vectors...tools for optogenetics and chemogenetics on the Addgene website. Optogenetics also requires that light ...brain. One way to address this is by working with organisms with light accessible brains, such as zebrafish...bs.mie.2018.01.022 Additional resources on the Addgene blog Read more about optogenetics New to viral...

  17. Reflections and Looking Ahead

    Type
    Blog Post
    ...power of community in 2024 In 2024, your trust in Addgene helped unlock unprecedented scientific collaboration.... Even more remarkably, 1,160 labs from 522 organizations in 41 countries enriched our repository with...online and in-person, you helped us understand how Addgene can better serve the global scientific community...about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez...

  18. What's New in CRISPR - March 2020

    Type
    Blog Post
    ... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR.... Caulobacter crescentus is a popular model organism for studying the bacterial cell cycle, asymmetric...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by...

  19. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology Pages The problem: Host binding proteins...membrane on an agar plate selective for your target organism and incubate overnight according to appropriate...on hand that will specifically bind your target organism, it can be helpful to spot this protein directly...PubMed Central PMCID: PMC2258639. Resources on the Addgene Blog Learn How CRISPR Can Be Combined with Phage...CRISPR For Genome-Wide Screens Resources on Addgene.org Visit Our Microbiology Pages Use the FX Cloning...
Showing: 221 - 240 of 449 results