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We narrowed to 466 results for: organization Addgene

Showing: 221 - 240 of 466 results

  1. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...entirely. Auxotrophic selection relies on the host organism being unable to synthesize a nutrient required... to design your vector to be functional in the organism your experiment takes place in. In many cases,...vectors a success!   Resources Resources on the Addgene blog Plasmids 101: Transformation, Transduction...Plasmids 101: The Promoter Region Resources on Addgene.org Collection of empty plasmid backbones ...

  2. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...generated using random genomic fragments from the organism of interest, or a defined cDNA library (e.g. the...number of two-hybrid systems available in model organisms, including bacteria, alternative fungi (C. albicans... Pubmed Central PMCID: PMC514463. Resources At Addgene Browse Our Yeast Expression Vectors Find Bacterial...

  3. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...specimens and for in vivo imaging of transparent organisms. Advances in tissue clearing Similar to light ...3DISCO (3-dimensional imaging of solvent cleared organs) method of imaging rodent brains and spinal cords...).  These viral plasmids are available through Addgene.  The combination of LSFM with multiple tissue ...

  4. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...your host organism. Because transcription machinery differs between cell types or organisms, promoters...writing of this article. Additional Resources on the Addgene Blog Browse All Plasmids 101 Posts Learn about...GFP Resources on Addgene.org Find Plasmids for Your Research     ...  Kendall Morgan...

  5. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...discussion, we bring up a performance Ben and Niroshi organized for Tyler's (the host's) thesis defense. This ...Additional Resources on the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research...Process  Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your...

  6. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...for Scientists Resources on Addgene.org Learn about Careers at Addgene ...accordingly. Read Additional Career Posts on the Addgene Blog How did this change in labor laws come about... salaries (which have been made by multiple organizations across academia). Increased postdoc wages, overtime... salaries as their website implies. These 5 organizations, according to the NSF data, account for 18% ...Research @FORsymp.   Additional Resources on the Addgene Blog Read about the 2015 and 2016 Future of Research...

  7. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...region (approximately 500bp is best) and select the organism. It will then tell you the available gRNA targets...can also find many previously validated gRNAs at Addgene. In vitro screening for the best gRNA Figure...Find Plasmids for Testing Your gRNAs in vitro at Addgene CRISPR/Cas9 gene editing in mice Now that you’ve... much more from our favourite mammalian model organisms in the near future.  Many thanks to our guest...PMCID: PMC3969854. Additional Resources on the Addgene Blog Get the Basics of Mouse Genome Engineering...Homology Directed Repair Additional Resources on Addgene.org Find Software Tools to Help Design You gRNA ...

  8. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...and stably maintain the genes they carry among organisms belonging to many different phylogenetic groups...bacteria and express proteins within different host organisms. pBBR1-based plasmids and plasmids in incompatibility...  Find broad host range recombinant vectors at Addgene!   References and resources References Doran, ...10.1007/s13205-023-03507-0  More resources on the Addgene blog Plasmids 101: Origin of Replication Plasmids...

  9. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...Blotting Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based technique, validation...you are confident, especially if you work in an organism that is underrepresented in the biomedical research...10.1038/nmeth.3995 Additional Resources on the Addgene blog Learn about research applications of antibodies...

  10. CRISPR Activation: A Practical Guide

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    Blog Post
    ...over-express coding sequences from a given cell type or organism, have been used in a similar manner to Cas9 activators...lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3) and organisms (Chavez et al., 2016). To learn more about the...    Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory at Columbia...doi.org/10.1038/nbt.3149 Additional Resources on the Addgene Blog Learn how to Choose the Best Cas9 Variant... CRISPR Has Been Used with AAV Resources on Addgene.org Find Cas9 Activators Find Cas9 Repressors Catch...

  11. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...plasmids with Addgene. Additional control plasmids for the TKO library will be available at Addgene soon. Hart...cellular compartments (cytosol, Addgene catalog #71245; mitochondria, Addgene catalog #71247) Rosella provides... in vitro cell systems and diverse model organisms. Addgene’s newest TALEN kit, the FusX assembly system...read? Listen to our hot plasmids segment on the Addgene Podcast. View Plasmids from:  March (Q1) | June...sequencing. The STARR-seq backbone is available from Addgene in both human and fly versions. Arnold, et al....alongside a growing number of CRISPR libraries from Addgene’s repository that can be used to perform screens...PMID: 24395366 Oxford Drosophila CRISPR library Addgene has had the privilege of distributing several human...

  13. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...plasmids you use to bring this into your target organism. We have designed a Golden Gate system based on...The complete GreenGate toolkit, now available at Addgene, also gives the option to stack several expression...motivations for making GreenGate available through Addgene,” Lohmann said. “We strongly believe the free sharing...  Kendall Morgan...

  14. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Along these lines, Darrell recently joined the Addgene Board of Directors where he hopes to help keep ...of this technique will someday allow us to make organs using iPS cells derived from patients. Chapter ...Chapter 12: Darrell's goals as a member of the Addgene board of directors - increasing open source sharing...Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids from the...

  15. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...protocols Sep-OTSλ plasmid - Addgene # 68292 C321.ΔA bacterial strain – Addgene #68306 pCRT7 NT TOPO E17TAG...E17TAG GFP plasmid - Addgene #68295 SupD plasmid – Addgene # 68307 For further technical reference and protocols...amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA synthetase, despite...flavors’ of Sep-OTS plasmids are available through Addgene, but we have worked most extensively worked with... recombinant phosphoproteins’ available on the Addgene website. Many thanks to guest blogger Natalie ... Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156...Protoc. doi 10 (2008). Journal. Resources on the Addgene Blog Verify Your Constructs with Colony PCR Read...

  16. Viral Vectors 101: Systemic Capsids

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    Blog Post
    ...plasmids available from Addgene Capsid Published Tropism Model Organism Mouse Strain-Specific...administration, distributing widely throughout the organism to target large or diffuse biological structures...good option for your experiment? Look it up in Addgene’s Data Hub and the CLOVER repository of published...specific application and animal model.  Other model organisms Tropism and efficacy can vary depending on species...target cell type or tissue of interest, and model organism under study. *Many engineered AAVs for mice use...most commonly requested systemic capsid in the Addgene repository. Like its parent AAV-PHP-B, it can be... and shows decreased transduction in off-target organs (Goertsen, Flytzanis, et al., 2022). AAV-PHP.N ...

  17. Top 10 Open Science Developments of 2013

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    Blog Post
    ...should come as no surprise that those of us at Addgene believe in the power of sharing, and especially...ban gene patents in a case against Myriad, an organization called Free the Data launched a public database...And, last but not least (certainly not for us), Addgene launched this blog, A Better Way to Share Science...  Kendall Morgan...

  18. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    Blog Post
    ...Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0, 1.1, 2.1 plasmids...addition to the neuronal pThy1-Brainbow constructs, Addgene also has two Brainbow adeno-associated viral vectors...techniques are also being applied to study model organisms such as Drosophila and zebrafish. Brainbow in ...important insights into the complicated physical organization of the brain and other biological systems. Interested...

  19. Caltech Systemic Capsids

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    Collection
    ...Ready-to-use AAV available from Addgene's viral service. Engineered systemic constructs from Viviana ...As part of our Viral Vector Packaging Service , Addgene is distributing ready-to-use viral preparations...target cell type or tissue of interest, and model organism under study. *Many engineered AAVs for mice use...depth descriptions of each serotype, check out Addgene's blog on Systemic Capsids . To review performance...data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER Center’s repository...produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB tropism and specific ... produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006) . Browse Available PHP.S AAV ID Name Promoter...

  20. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Reading and Resources Addgene Blog: Which Fluorescent Protein Should I Use? Addgene Blog: Choosing Your ... to help you plan your experiment. Tables are organized by color of the fluorescent protein. The content...covalently bind a synthetic substrate conjugated to an organic fluorophore or other small molecule. By using different...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...
Showing: 221 - 240 of 466 results