... for entry of the antibodies. Here, a two-step process is employed: the cells are first fixed and then...phosphatases, and ubiquitin ligases; and many more. This process, however, leaves the nucleus intact. If markers...not need to use a separate fix/perm cytosolic procedure in that case.     Figure 3: For intracellular...

...heterogeneous cell population, for example from a processed piece of tissue, where cells are present that ...structural complexity. After several steps of tissue processing prior to running my flow analysis, I can however...interest). Let’s double-click inside our gate and proceed to the next plot! Pro tip! If you only get very...

...body, integrins are key to a diverse array of processes, including cell adhesion, migration, and signaling...investigate the role of specific integrins in cellular processes.   Figure 1: Integrins can shape into three...things IPI, from the scientists and scientific process behind the scenes to the new antibodies and research...

...doesn’t, and trying all over again. Here’s that process!   Figure 1: The Design-Build-Test-Learn...gear, and walking everyone through the recording process was going to be time consuming and difficult. Finding...in the future, but we were finally refining the process down to a place I could be happy with it. Cycle...

...These biotin dependent enzymes are essential for processes such as gluconeogenesis, lipogenesis, amino acid...energy transduction. Biotinylation is the general process by which biotin is added to a protein or macromolecule...biotinylation occurs naturally in cells and is the process by which biotin is covalently bonded to a specific...

...great way to stimulate and study these physical processes. Joachim Goedhart and colleagues in the Gadella...could be applied to study other actin-dependent processes in cells and tissues. And, this Opto-RhoGEF design...the protein of interest supports a variety of processes in different cell types. One great way to do this...

...that can arise during the plasmid purification process. One is the possibility of damaging the plasmid...carrying over contaminants from the plasmid prep process into the final plasmid eluate. Possible contaminants...contaminants can sneak in from almost any step of the process and include things like genomic DNA, RNA, proteins...

...inefficiency? Use CRISPR to make it a selectable process! As NHEJ is ineffective in bacteria, CRISPR-induced...collection of organisms, their genes, and metabolic processes, occupying the gut. Many human diseases have connections...properties of CRISPR-Cas systems is base editing. This process is generally accomplished by fusing an enzyme capable...

...steps in the viral production and quality control process to improve both the yield and quality of the viral...scientists with the means to study biological processes on the cellular level instead of having to work...

.... Listen to this episode for insights into the process of getting an MD PhD, the inside scoop on developing...family. 3:56 - 7:04 : Alex describes the MD PhD process and explains why he loved the first 2 years of ...

...experiments - and we've updated our submission process so you can easily add your data, too!   Using...should!) submit your data to the Data Hub! The process is easy with our new submission form - and no worries...

...antibody worked in your application. The submission process is easy, with simple, descriptive fields, drop-..., and can be saved at any point throughout the process, so you can work through it at your own pace. To...

...Improvements that can be made to the grad school process 22:41 - END : Niroshi and Ben's favorite advice...Future of Research Looks to Improve the Research Process  Browse our Career Posts Resources on Addgene....

...scientific sharing. So we set out to streamline this process. We take pride in facilitating the exchange of ... and by whom. For Recipients: Streamlined Processing: When you place an order, Addgene generates the...

...of optogenetic control of endogenous cellular processes." The LITE system can now be applied to unraveling...genetic and epigenetic players in normal biological processes and disease states. While LITE plasmids have clear...

...The SITN Editorial Process -Vini discusses the importance of the editorial process and peer-to-peer feedback...

... be expensive and time-consuming to repeat the process with these new photoconvertible proteins. With ...state after excitation at 561 nm. This imaging process worked well with the common GFP variants EGFP, ...

...Homologous recombination is the process by which nearly all domains of life repair genomic damage, specifically...Researchers have long taken advantage of this natural process to integrate protein tags into the genomes of S...

...Knowledge-Base, seeking to create an iterative process that is constantly becoming better, with more data...spatial analysis of cells in complex tissues. Proceedings of the National Academy of Sciences, 117(52),...

... original publication. To help facilitate this process, we explain how to cite plasmids from Addgene right...much information as possible during the deposit process. Special collections pages In an effort to make...