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We narrowed to 535 results for: val

Showing: 221 - 240 of 535 results

  3. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...transposons open whole new worlds to explore. The newest arrival on the scene is called evoCAST — an evolved CRISPR-associated...individualized optimizations, safety testing, and approval. In contrast, a system that can deliver a healthy...

  4. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...reproducibility of scientific data depends on validated reagents along with clear documentation, so we...programatically, their API allows fine grained query and retrieval of all the information in the resource.  This ...

  5. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...). The original plasmid is empty following the removal of the transposon; the plasmid is then degraded...chimeric antigen receptor (CAR) therapy. SB is a valuable tool and an alternative to CRISPRs for genome ...

  6. Advice for Choosing a Research Project

    Type
    Blog Post
    ...especially you!) care about. Have you critically evaluated your plan? Have you read the relevant literature... doing the same experiment for too long. Keep evaluating your project along the way. Advice from principal...

  10. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...educational materials, protocols, and shared data, on our values of transparency, reproducibility, and community-building... use in brain imaging (Andrews et al., 2019), validated in both western blot and immunohistochemistry....

  11. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...robust expression of high G+C content genes, as validated through monitoring the expression of fluorescent...sensors New AAV tools to selectively control parvalbumin interneurons from the Dimidschstein Lab More ...

  13. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...Myc was developed in 1985 and is still the most prevalent clone used today! Goat, chicken, mouse, or rabbit... brought about by the tag so it’s important to validate tagged protein functionality (Arribere, et al)...

  14. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ... the outcome of the project, or illustrates the value of the work and then build a story around them. ...way at the bottom of my story” and that’s a very valid critique! But do you remember earlier when I said...

  16. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...small capacity viral vectors such as AAV. Base removal RNA-guided CRISPR enzymes cause double-stranded...a natural context, it’s hypothesized that base removal helps prevent the invading genome from “recovering...

  17. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...successfully combine CRISPR and AAVs. 9. CRISPR 101: Validating Your Genome Edit Once you’ve used CRISPR/Cas9...Melina Fan guides us through the basics of the validation process for CRISPR mediated genome engineering...

  18. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...localization. Thus, the usefulness of CPPs must be evaluated on a case-by-case basis. While setting up their...retained their stemness, indicating that iTOP may be valuable for CRISPR/Cas9 stem cell editing. The ...

  19. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...Control AAV For many AAV experiments, we recommend validating the serotype in your target cells before conducting... wide array of promoters and fluorophores for validating and testing purposes. We will continue to add...
Showing: 221 - 240 of 535 results