CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
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More CRISPR-Cas Blog...reprint of the CRISPR article "Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9" from...Orkin and Daniel Bauer's labs details the use of CRISPR/Cas9 to create genomic deletions in mammalian cell...discuss the motivations behind this research.
Using CRISPR/Cas9 for targeted genomic deletions
We were inspired...collaboration with the Porteus lab [2]. With the advent of CRISPR/Cas9, we began to explore the paired double-strand...bear an indel or inversion mutation [3].
In the CRISPR era, the genomic landscape may be surveyed in unprecedented... procedures. The video details how to transfect CRISPR plasmids into MEL cells by electroporation, sort...