Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 284 results for: cas9 plasmid

Showing: 121 - 130 of 284 results

  1. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... eSpCas9(1.1), or HypaCas9. Read more about Cas9 options in our blog post here. Dealing with low editing...version of Cas9 that has been specifically engineered for reduced off target editing, such as SpCas9-HF1, eSpCas9...gRNA and introduced it into your target cells with Cas9. Hooray! Now it’s time to make sure your genome ... product of both an unedited population and your Cas9-targeted cells. Simply upload both trace files to...with the estimated editing frequency of your sgRNA-Cas9 combo. Pro tip: When designing your PCR, ensure ...introduced a donor template along with your gRNA and Cas9 combo and are hoping for homology-directed repair...despair, you have options! If you think delivery of Cas9 or gRNA is the issue, check out or blog on optimizing...

  2. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...editors use either catalytically dead Cas9 (dCas9) or a Cas9 nickase (nCas9) to avoid introducing double-strand...need from Cas9 are sequence specificity and the R-loop structure. In order to bind DNA, Cas9 requires ...non-target strand of DNA for Cas9 to bind.   R-loop The structure formed by Cas9 binding to DNA and separating...CRISPR 101: Cas9 Structural Biology Additional resources on Addgene.org Browse CRISPR plasmids for base...PAM) on the opposite strand. Once it finds a PAM, Cas9 unwinds the adjacent DNA to create a region of separate...single-stranded DNA (ssDNA) region, using enzymes fused to Cas9 to alter the exposed ssDNA bases. Thus, base editors...window restricted to the stretch of ssDNA created by Cas9 binding. Pro tip! In CRISPR, the non-target strand...

  3. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ... to use variants of Cas9, like split Cas9, or a smaller Cas9 ortholog like SaCas9. You could even venture...to use a multi-plasmid system, where you have one AAV vector dedicated to expressing Cas9, while the other...at double-stranded break points caused by CRISPR-Cas9 editing. The good news is that this integration ...approximately 4.7 kb — which means that when using Cas9, you’re already almost out of space! Don’t let this...venture away from the comfort of Cas9, and opt for a completely different Cas effector, like Cas12a (Cpf1...

  4. Design Tips for Prime Editing

    Type
    Blog Post
    ...the CRISPR guide Find CRISPR plasmids by function Browse our entire plasmid collection ...editing technique that relies on a fusion enzyme of Cas9 nickase + reverse transcriptase and a prime editing...., 2019), disrupting its canonical structure and Cas9 binding (Nishimasu et al., 2014). Figure...pegRNAs that use other bases in this location bind to Cas9 with canonical sgRNA structure (right). Created ...along with your intended edit. This prevents the Cas9 nickase from re-binding and nicking the newly synthesized...Zhang, F., & Nureki, O. (2014). Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA. Cell, ...

  5. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse Models with CRISPR/Cas9 Additional...techniques, please see “Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity” by Tycko et al. 2016...improving the genome-wide specificities of CRISPR–Cas9 nucleases”
by Tsai and Joung 2016. Many thanks ...high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations using next-generation sequencing...of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity." Nature biotechnology 31.9.... "Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells." Nature methods...Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral ...

  6. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ... CRISPR plasmid can be created in a single tube. You can also find additional SapTrap plasmids used for...about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our Plasmids 101 blog posts Resources on Addgene.org...discussed at the meeting, in addition to useful plasmids and toolkits available at Addgene.  C. elegans... development (Kyoda et al., 2012).  C. elegans plasmid tools at Addgene In addition to the wealth of open...open resources described above, there were many plasmid tools presented at the C. elegans meeting. Here... on new resources and tools for the community. Plasmids for the Auxin Inducible Degradation System (AID...and temporally knock-down proteins of interest.  Plasmids for cGAL and split cGAL systems The Sternberg ...

  7. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...EsCas13d. Find the Yan et al. plasmids here Find the Konermann et al. Plasmids here How do Cas13d enzymes...than other Cas13s and a whopping 33% smaller than Cas9! Their small size makes then easy to package in ...significant off-targets, CasRx displayed zero. Unlike Cas9, for which efficiency varies widely across guide...S0092-8674(18)30207-1. PubMed PMID: 29551272 Plasmids from this paper are available at Addgene. Yan...S1097-2765(18)30173-4. PubMed PMID: 29551514 Plasmids from this paper are available at Addgene. Learn...CRISPR RNA Editing with REPAIR Get tips for using Cas9 Transcriptional Activators Learn about Cas13a (C2c2... Resources on Addgene.org: Find RNA Knockdown Plasmids Check out our CRISPR Guide Read our CRISPR History...

  8. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. ...Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set for expressing tagged proteins...and AAV plasmid sets launching later this year!  Find Addgene's own Lentiviral backbone plasmids here! ... synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A., Negrean, A., Chen, Y., Iyer...powerful tools in a scientist's toolkit is the empty plasmid backbone: the DNA vector that contains everything... swap sequences effortlessly.  We built these plasmids for flexibility and speed, so you can design, ...Bentley For RNA-guided genome editing, think beyond Cas9 with STITCHR: a combined CRISPR / retrotransposon...

  9. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...changes we’ve made: New CRISPR plasmid pages We’ve given our CRISPR Plasmids and Resources a fresh new design...design, sorting the plasmids we have available by category. Each plasmid page now has a helpful graphic...introduce you to the plasmid class covered on that page. To help you find popular plasmids, we’ve also added... new plasmid pages that cover some of the hottest CRISPR tools. Base Edit: Base editing plasmids allow...libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids 101: NGS Quality Control for Pooled Libraries...revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that’s not our only job - we strive to also...up with new developments (and, of course, find plasmids that will be useful to you.) With these goals...

  10. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...J, Collins JJ, Church GM (2015) Highly efficient Cas9-mediated transcriptional programming. Nat Methods...Perrimon N, Collins JJ, Church G (2016) Comparison of Cas9 activators in multiple species. Nat Methods 13:563...transcriptional activation by an engineered CRISPR-Cas9 complex. Nature 517:583–588 . https://doi.org/10.1038...Gersbach CA (2013) RNA-guided gene activation by CRISPR-Cas9–based transcription factors. Nat Methods 10:973–...Addene.org Read Addgene's CRISPR Guide Find CRISPR plasmids for your research ... cell lines.   dCas9-VP64 Description CRISPR activation can occur through fusing dCas9 with VP64, a strong... for CRISPR-based gene activation. dCas9-CBP In this method, dCas9 is fused to CBP, an histone acetyltransferase...
Showing: 121 - 130 of 284 results