We narrowed to 399 results for: MUT
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TypeBlog Post...transduction with this virus. While various Cas9 mutants have been engineered to perform unique editing ...
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Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...essentially any gene,” she says. The technical leap from mutating and deactivating a gene or genes to selectively... -
Rett Syndrome
TypeCollection...caused from loss-of-function mutations in the MECP2 gene. Causative mutations for Rett syndrome typically...within patients with the same mutation, the location and type of MECP2 mutation can be a strong predictor ...missense mutations located in the MBD, and truncations prior to the NID are more severe than mutations further...to their laboratory website. Cell Line Mutation (DNA) Mutation (protein) Sex Source of Material Publication...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...meet diagnostic criteria have disease-causing mutations in the gene methyl-CpG binding protein 2 ( MECP2... expressing wild-type MECP2 and half expressing mutant MECP2 . Skewing of these X inactivation ratios ... -
Plasmids 101: The protein expression toolbox
TypeBlog Post... gRNA is all you need to generate a frameshift mutation that will permanently ablate protein expression... -
All in a Twist: dsRNA
TypeBlog Post...inflammatory response. However, if dsRNA sensors are mutated or mechanisms which suppress endogenous dsRNA formation... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post...engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes are worth... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...early-onset cataracts, is characterized by point mutations in the Iron Response Element (IRE) RNA motif of... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog Post... the protein. To monitor autophagy, the Pho8Δ60 mutant protein is expressed. Pho8Δ60 localizes to the ... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...light-sensitive protein QuasAr2 and systematically mutated it. Using a robotic picking approach to select ... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...domain of a Ciona intestinalis voltage sensor and a mutated version of the pHLuorin fluorescent protein. This... -
Antibodies 101: Affinity Reagents
TypeBlog Post...fragments. However, rigidity can be introduced through mutation, allowing diabodies to be used for assembling ... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...lung cancer cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post...amplification can be used to create silent point mutations at internal recognition site(s) thus eliminating... -
Plasmids 101: Cre-lox
TypeBlog Post...potentially recombine. To account for this, alternate mutant versions of the loxP site have been created, which... -
Overcoming the Challenges of Lentiviral Production
TypeBlog Post...promoter of the 5’ long terminal repeat (LTR) is mutated in third generation systems such that it no longer... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post... assays. ShadowG is darker than other dark YFP mutants, it reduces the risk of spectral contamination ... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...now circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...it’s also good to make a few single base pair mutations in crRNA target sites found within the homology... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog Post...deletions, but CRISPR/Cas9 can also introduce point mutations. While using targeted nucleases to generate an...