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  1. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol. Rev. 90:1103...et. al. (2011) “Modern fluorescent proteins and imaging technologies to study gene expression, nuclear ...

  2. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...fluorescent label, which can reduce the potential image resolution of super-resolution fluorescence microscopy...Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex...

  3. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...   Figure 2: Structure of a retrovirus. Image re-used from Dong & Kantor, 2021 under CC-BY license...   Figure 3: The life cycle of a retrovirus. Image courtesy of Jeremy Luban, MD. Figure 3 depicts...

  4. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ... appear darker than full vector particles. This image shows that the vast majority of the vectors consist...particles (black arrowhead). Scale bar = 100nm. Image courtesy of David Bell and Svetla Stoilova-McPhie...

  5. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...groups have utilized dCas13 fusion proteins for imaging, tracking, modulating splicing, and regulating ...fluorescent fusion protein dLwaCas13a-NF for in vivo RNA imaging. In short, the Cas13a molecule brings a much anticipated...

  6. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...would help you do the job as described. Let’s imagine you’re applying to a job that requires ddPCR skills...answer to that is what goes into your cover letter. Imagine you’re applying to a job with a lot of file management...

  7. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...knock in, base edit, activate, repress, and even image your favorite gene. With so many Cas9 reagents available...target strand based on the sequence of the pegRNA. Image from David Liu.   Activation and repression...

  10. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...with mRNA and charged tRNAs to build a peptide. Image from Mariana Ruiz Villarreal. By the late 1980s... codons are under expressed compared to others. Image from Hiss et al., 2017. Most commonly, scientists...

  11. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...schools. I will emphatically deny that I have a magic formula to guarantee a reader the faculty job of...optimization of fluorescent proteins,  and live cell imaging approaches. Erik is also an avid long distance ...

  12. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...now to capture interesting key results, striking images, creative ideas, and engaging stories as you encounter...supportive audience? 7. Hone Your slides Use big images that are easy to see (and for heavens sakes, label...

  14. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...aureus Cas9 (Sa-Cas) and deposited plasmid IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly.... These two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9 fusions for...

  15. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...5, 6, 8, and 9 in two different brain regions.  Image modified from Aschauer et al., 2013, Figure 1. Used...both oligodendrocytes (Olig2) and neurons (NeuN). Image from Griffin et al., 2019, Figure 4. Used under ...

  16. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ... is twice the length of the monomer (~11.6 kb). Image generated by SnapGene software.  Thanks to ...multimer identification.   Fig. 2: Gel image of Plasmid 61564: pBAMD1-2 (~4.7 kb) digested with...

  17. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...paper. Pretty much like an actual sandwich! S'mores image from Evan Amos.   After the transfer, some areas...chemiluminescence, is detected on x-ray film or with specific imaging devices. In most cases, the primary antibody, which...

  18. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...cellular repair mechanisms rush in to repair the damage. If all they can do is stitch the two broken ends...Mizutani E, Sato H, Kasai M, Ogawa A, Suchy F, Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette...

  19. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...cargo with the excision-only piggyBac™ transposase. Image courtesy of Transposagen.   How does piggyBac... selection cassette by excision-only piggyBac™. Image courtesy of Transposagen.   How does the piggyBac...

  20. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...and nearest sample. To minimize the risk of DNA damage, it is best to limit the UV exposure of the DNA...DNA. Therefore, it is a bad idea to use a gel imager to take a picture of the gel before cutting out the...
Showing: 241 - 260 of 468 results