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Showing: 361 - 390 of 954 results

  1. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ...bacteria. Learn more about the different CRISPR applications in plants.     Transgenic yeast as source of...Vietnam Linh Bao Ton (center) is interested in applying gene technology to bacteria and plants. Her research... water repositories in Mexico. At Addgene we're happy to be able to support diverse groups of scientists...collaborations. Many thanks to all of the awardees, all who applied, and to Seeding Labs who helped us coordinate and...

  2. Hot Plasmids - October 2022

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    Blog Post
    ...a custom, high-throughput screening platform to apply saturating mutagenesis on 21 positions in the protein...in plasmid form. Fig. 1: Summary of the approach and outcomes of the screen for improved genetically-encoded...blot, but we are actively testing it in other applications! If you try it out in a new assay, let us know...GFP-smFP-myc expressed from Plasmid 98926. See the Applications section on the Anti-c-Myc [9E10] page for more...

  3. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...postings looking for PhD applicants since many will also accept applications from master’s candidates ...technical or product support specialist,” “field application scientist,” or “scientific or technical writer...Principal Scientist or Scientist I/II. Promotions don’t happen overnight though, and it might take longer to advance...

  4. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ...defining a couple of key terms is a better approach. Also, appealing images and colors draw people in. Drawing...should be. He debated taking a position as an application support/sales representative in industry or a...illustrations for summarizing mechanisms and applications that are relevant to things IDT does. I also...

  5. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...types of yeast expression vectors. Consider your applications and experimental design. You can create a new...shuttle vectors. You may prefer the more traditional approach of an autonomously replicating vector that carries....g. pMT-puro). Worm vectors There are several approaches to studying YGOI's function in worms. Some of...a variety of other types of vectors for most applications used to study gene function. From making transgenic...

  6. Advice for Choosing a Research Project

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    Blog Post
    ...project that combines an exciting new method or approach with another in a way not done before in your ... - maybe something used in astrophysics can be applied to fungal biology.” Connie Cepko advises students... technology to identify a problem, asking what approach will I take and what can I learn, learning the...importance of choosing a solid research topic and approach. At Addgene, we've heard of labs where new hires...

  7. History of CRISPR Cas - A tale of survival and evolution

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    Blog Post
    ...proteins snip the foreign DNA into small fragments approximately 30bp in length and paste them into the CRISPR...various species and describe an assortment of applications. Despite the ubiquity of CRISPR/Cas toolkit,... multiple plasmids used for various platform applications. For more information on CRISPR/Cas, check out...for your research. Do you have a cool idea or application that involves CRISPR/Cas technology? Let us know...

  8. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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    Blog Post
    ...teamwork and initiative it took to make all this happen with 80% of the team working remotely. We’re extremely...capacity to serve the scientific community. First, we applied for the government sponsored Paycheck Protection...a miraculous grant from Fast Grants, which was approved in a matter of days because they grasped the crucial...on track to succeed at the same time. I began approaching science funding organizations to request support...

  9. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...TA-based ligation and tagmentation preparation approaches described previously require a preliminary double...that could be used as a template for downstream applications (Berns, 1972). Using qPCR and restriction enzyme... sequencing workflow. Using this simplified approach, we sequenced over 100 AAV vector preparations...believe that this simplified sequencing and analysis approach can be easily incorporated into standard QC regimens...

  10. The Golden Stopwatch Award: Recognizing Facilitators of Research

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    Blog Post
    ... transfer resources (summaries, electronic MTA approval, archive of old MTAs, and more) Does your TTO ...periodically for possible new awardees, and we’re happy to share stats and strategies with tech transfer...executed between a TTO and Addgene, whose terms apply to all future deposits of materials with Addgene...executed between a TTO and Addgene, whose terms apply to all future materials requests from Addgene MTA...

  11. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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    Blog Post
    ...opportunity to study it and see what happens when technological change happens really fast,” Thompson says. To...of sequence data. A group of scientists from MIT tapped into this data to learn about trends in synthetic...have generated robust tools that have practical applications in monitoring the accidental or intentional ...

  12. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ...makes them more adaptable to a broad range of applications. Recently, the Spiess lab developed a set of...Institute focuses on proteomics: developing and applying new technologies to quantify proteins in tissues...Scientist in the group, developed a CRISPR-based approach to detect proteins that associate with specific...used to label all proteins within a radius of approximately 20 nm of a specific DNA sequence in only 1 min...

  13. The Strength of Story Telling

    Type
    Blog Post
    ...all things almost all of us are programmed to appreciate and understand. We build narratives around the...readers or listeners thus expanding the impact and appreciation for their research. Long story short, telling... to date, invite people to ask questions, be approachable. Engage with your audience and try to learn ...clear what was difficult and what was easy when appropriate. It might not be scientific to bring emotional...

  14. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ... “species on species”) immunochemistry (IHC) applications an issue? The culprits are endogenous IgGs in... same species as your primary/sample, and then applying this mixture to your tissue (Tuson, et al., 1990...After washing away unbound fragments, you can then apply your primary and secondary antibodies as normal....Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to Mouse Tissue...

  15. Viral Vectors 101: Transductions

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    Blog Post
    ... typical genetic cargo — the viral genome — is swapped, at least partially, for a nucleic acid cargo of...since the majority of the viral genome has been swapped for alternative genetic cargo, they can’t reproduce...viral preps available for a variety of broadly applicable and popular viral vectors. Check out our viral...should you opt to make a virus instead of just zapping your cells? That really depends on two things. ...

  16. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ... unexplored. Using new single-cell sequencing approaches, the lab was able to eliminate the need for axenic...Quake Lab, volunteered to do the sampling and I happily agreed to accompany him to YNP the following year...a thermostable Cas9 be useful? It can enable applications where reagents must be stored at elevated temperatures...stability under harsh conditions. One example application from the publication is the removal of unwanted...

  17. Addgene's Top 10 Blog Posts of 2015

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    Blog Post
    ...revolution in biological research. We’re incredibly happy to see that one of our career posts has made it ..., we hope we can help all researchers achieve happiness in their careers regardless of their end goals...addgene.org. Thank you again for a fantastic year and happy reading! Top 10 blog posts of 2015 1. CRISPR 101...some common controls and why you might need them. Happy Reading!   Resources on the Addgene Blog Download...

  18. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...parasites, to symbionts, to genes and the labels applied to them were vague or contradictory, owing in part...elements. At the time, the use of episome seemed appropriate, especially since the Fertility, or F-factor ...seen as an ubiquitous tool that can be diversely applied to many different experiments. Addgene was founded...Vectors" chapters, and more pictures and pro tips.  Happy reading! Learn more about the history of plasmids...

  19. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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    Blog Post
    ...Creating a modular, selection-based gene tagging approach Multiple features of C. elegans have made CRISPR...permission from the Genetics Society of America. Applications of this system Dickinson et al. have developed... in cell culture. In addition to CRISPR/Cas9 applications, SEC selection could replace other, more restrictive...Additional Resources on the Addgene Blog An “elegans” Approach to Better Cas9 Editing Efficiency CRISPR 101: ...

  20. Scientists Map the SARS-CoV-2-Human Interaction Network

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    Blog Post
    ...millions, if not billions, of dollars, but by approaching it from a new direction, we hope to do it much...Guo, Fengbo Zhou, Paddy O’Leary (UCSF). Our approach is to discover key human proteins that can be ...therapeutics that are safe, effective, and already FDA-approved). This is a big job, but under the leadership ... half the drugs hitting these proteins are FDA-approved or in clinical trials or preclinical studies already...

  21. New and Upcoming Viral Vectors - September 2019

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    Blog Post
    .... For a single action potential, GCaMP7s is approximately twice as bright, relative to baseline fluorescence...fluorescence, as the GCaMP7f variant, and is approximately 5-fold brighter than GCaMP6s. jGCaMP7f is the fastest...inactive neurons is reduced. This variant has an approximately 12-fold increase in brightness after ~100 action...genetically-defined neuronal populations with intersectional approaches is a growing field that we aim to support by ...

  22. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    ...research. This year, we put out another call for applications. We had an overwhelming response and were excited...many creative uses of fluorescent proteins. We’re happy to announce the three winners of this year’s Michael...does that mean? Previous research shows that approximately 75% of all protein-coding pathogenic human variants...artificial leaf replica system. “This bottom-up approach will allow me to create a fully controllable and...

  23. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...erupted in controversy. Briefly, there are two overlapping, valid critiques being offered of He’s work: ...public involvement, research, and consensus can happen. We just have to look to the United Kingdom’s Nuffield... technique was feasible, three separate things happened nearly simultaneously: The Wellcome Trust funded...biosecurity, and the entire 2018 saga of “what’s happening in genetics this time?” She lives with her partner...

  24. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ... related to PZA-resistance by using different approaches, including CRISPR and recombination-mediated ...fluorescence microscopy and FACS. In an additional approach, plasmids such as pACBSR-hyg and pFLP-hyg will... the river back to the user.   At Addgene we're happy to be able to support diverse groups of scientists...collaborations. Many thanks to all of the awardees, all who applied, and to Seeding Labs who helped us coordinate and...

  25. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...characterize a protein of interest (and we very much appreciate even those small contributions). Of course, no...incorporate FPbase data in their own projects and applications, a REST API allows programmatic retrieval of...within-study photobleaching comparisons.  We very much appreciate feedback, so try out the site and contact us ...Techniques - Which is best for me? A Practical Approach to Choosing the B(right)est Fluorescent Protein...

  26. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...in pairing with base editing and prime editing approaches. Recently, Xue Sherry Gao’s lab at Rice University...packaged into viral vectors for potential in vivo applications or multiplex CRISPR screening.   Using tRNAs...and efficient in plasmid and viral vectors. In applications when repetitive tRNA sequences must be avoided...construct DAP array for multiplex gene editing applications, we provide detailed manual and online design...

  27. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ..., or reordered and PCR enzymes are adjusted. Appropriate fragment and primer design results in covalently...transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi DNA Assembly Mix include...target gene, for example, are amplified with overlapping regions of homology and inserted into a linearized...associated with otherwise more complicated assembly approaches, and opens the door for new opportunities in ...

  28. Visualizing Translation at the Single Molecule Level

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    Blog Post
    ...fluorescent signal: untranslated RNAs appear yellow, and translated RNAs appear red. To enable this color switching...and RFP are bound to the reporter, causing it to appear yellow. The act of translation pushes NLS-PCP-GFP...tethers mRNAs to the plasma membrane, it’s not appropriate for use with mRNAs usually translated in a specific...

  29. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ... with an intuitive user interface.   The team approached the issue from multiple angles: the overall user...of information so we were able to fine tune our approach much more than if we were dealing with general...” variations and synonyms of the same term that appear in our data into a single filter value. For example...our search engine to be so specialized that it appears to “understand” biology.  We hope you will give...

  30. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post
    ...engineering E. coli metabolism, maybe you’ll be able to apply your knowledge of metabolism to better understand...favorite FRET-based biosensing technique can be applied to an important neuronal signalling pathway - if...got a useful tool, there’s likely someway you can apply it in neuroscience. AAV tools from Addgene are already...targeting genes to specific cell types in the brain, mapping neuronal networks, and selectively activating signaling...
Showing: 361 - 390 of 954 results