We narrowed to 794 results for: Tec;

... understanding and application of CRISPR/Cas9 technology are coming along at a fast and furious pace. ...Way to Edit DNA" and "This Protein Could Change Biotech Forever."  David S. Weiss of Emory University summed...

...molecules are very useful tools because we can easily detect and measure the light they emit. Proteins that ...closer look at how they’re used, see some new techniques that take advantage of them, and give some tips...

...Jennifer Tsang Prime editing is a new CRISPR technique from David Liu’s lab that gives more control and...into bacteria or eukaryotic cells is a common technique in molecular biology and scientific research. ...

... Genome-Wide Screening Browse 28 Hot Plasmid Technologies from 2015 Additional Resources on Addgene.org... Hot Plasmid Pages to See the Latest Plasmid Technologies ...

...assay, sequencing, RT-qPCR, or immunoblotting to detect. Another approach is to target two exonic sites...design, cloning, and delivery, as well as their techniques for creating deletions, identifying these deletions...

...They generated these editors using the BE4max architecture. The new editors reported in this study have...relative to BE4max.  Read the paper in Nature Biotechnology Find the base editor plasmids CRISPRi in Caulobacter...

...Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine. Neuron, 102(4), 745-761.e8....Fluorescent Biosensors Which Fluorescent Microscopy Technique is Best for Me?  In Living Color: The Skinny on...

... curator of the thousands of amazing plasmid technologies contributed by our depositors, Addgene strives...certainly not least, we often feature new plasmid technologies on our blog and encourage you to fill out this...

...Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids to study the expression of an unknown...plasmid sharing? Check out our Top 10 Plasmid Technologies blog post Or look at our "10 Years of Plasmid...

...theory or practice; business-like or artistic; tech, anti-tech or pragmatic.” Advice for pursuing careers...

...reported doing in a paper that appeared in Nature Biotechnology back in 2011 and then in another Nature Methods...protein for in vivo imaging. Filonov et al (Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918.) Near-infrared...

...also do videos demonstrating an experiment or a technique to students, like how to pour a gel [for] electrophoresis...really cool to take a step back and look at new technologies in education and ask ‘Can we implement that?...

...interview three science communicators who work in the biotech, education, or nonprofit industries. First up ... a job as a Science Writer at Integrated DNA Technologies (IDT). “It was fairly accidental. I was finishing...

...services we provide like quality control, curation, technical service and open-access educational resources....nonprofit? One big reason is that scientists and technology transfer offices trust us to work for them with...

...Chemogentic and optogenetic technologies have pushed the boundaries in neuroscience by granting targeted...activity. While they serve similar purposes, both techniques offer researchers different advantages and limitations...

...overcomes all of these labor-intensive and invasive techniques to reliably label any post-mitotic cells in Drosophila...et al., 2019. Applications for SPARC SPARC technology is versatile and can be applied across cell types...

...intertwined. In this blog we will review how CRISPR technology has brought drug and genetic screens together...of control have also been added to the CRISPR technology to allow time-controlled edits to occur, such...

...graduate student down the hall who got it from some technician downstairs who got it from who knows where), ...Cellosaurus, a Cell-Line Knowledge Resource. J Biomol Tech. 2018; 29(2): 25-38....

...sections. The focus groups allowed us to try out techniques that were discussed in the larger sessions and... Well what does that mean? If you start a new technique, then type up the materials and methods. Over ...

...learn about Synthetic Biology and exciting new technologies in the field. One of the main aims of the society...networking to promote the creation of start ups and biotech spin offs. The Synthetic Biology Society also aims...

...field. Advances in genome engineering & CRISPR technology That afternoon's workshop, entitled "Advances...amazement at how much has been done with CRISPR technology in just 2 years and excitement about how much...

...Their aim - to red-shift the boundaries of FP technology by shifting the emission maxima toward the red...However, a challenge in optimizing base editing technologies across several cell types is the lack of a rapid...

...STAR (Situation, Task, Action, Result) interview technique, which has been addressed in much better pieces...story should be too. Use drama Use any rhetorical technique you have in your arsenal to make sure people pay...

...postdoc association, started a speaker series or biotech club, managed their own blog or podcast, initiated...the board of a professional society, solved a technical problem, had an alter ego as a mad scientist for...

...scientists studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure that ...applied for the government sponsored Paycheck Protection Program loan and received enough funds to make...

...bacterial strains, such as Stbl3s. ITR loss can be detected by restriction digest using enzymes, like SmaI...Kamens J, Fan M (2019) Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation...

...containing lentiviral vectors the iBARs can be detected by next generation sequencing.  In order to avoid...measurements, or monitoring in vivo. To overcome these technical issues, Sabrina Büttner’s lab designed a calcineurin...

...reliably confirm the identity of AAV genomes and detect contaminating AAV DNA. In addition, we wrote an...minutes and the scripts  can be easily customized to detect other sequences of interest.   While the VGS process...

...The sky is really the limit for this emerging technology! Resources and References References Orguc, ...modulation of gut and brain neural circuits. Nature Biotechnology, 1–13. https://doi.org/10.1038/s41587-023-01833...

...secondary to detect Protein A and green fluorophore-tagged anti-mouse IgG2a secondary to detect Protein B...

...Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative, will allow Addgene to... The development of monoclonal antibody (mAb) technology circumvented many of these issues by creating...

...desired identity. The development of single cell technologies, such as single cell RNA-seq (scRNA-seq), now...multi-generational cell lineage trees. CellTags were detected over a 10-week period. Their expression decreased...

... genetically encoded antibody based probe that detects the widely used HA epitope in diverse cellular ...dynamics in vivo. In the future, this stitching technique could be applied to other epitopes and scaffolds...

... to detect mutants, but this method limits screening to a few hundred worms, making the detection of rare...

...especially for life science researchers. Many biotechnology companies are looking for PhD or Master’s level...capabilities, courses and office skills. I removed my techniques and protocols, courses taken during my PhD and...

...translational approaches for scientific tool and technique development. However, careers outside of the lab...Management Leadership Networking University technology transfer office positions   Market analyses...

...particularly interested in advanced in vitro models technology. Follow him on twitter @FRE_SCI.   Additional...resources Find protocol videos to teach basic lab techniques Plasmid Land: The plasmid cloning game ...

...viral particles per cell, you may not be able to detect AAV-mediated gene expression in a target cell. ...confirm. Luke Makana Hanley is a Research Team Lab Tech at Addgene working on methods to improve AAV production...

... to learn more about this killer illumination technique! What is a photosensitizer? First, for a few ...genetically encoded photosensitizer. Nature Biotechnology,24(1), 95-99. doi:10.1038/nbt1175. PubMed PMID...

... where the mutation can be placed. With other techniques, when mutations are spaced farther apart, it ...understanding the minimal genome. Many of these techniques require more complex DNA assemblies and improvements...